Experiment: Vancomycin 0.12 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2518 and Pf6N2E2_2519 are separated by 28 nucleotides Pf6N2E2_2519 and Pf6N2E2_2520 are separated by 13 nucleotides
Pf6N2E2_2518 - Cyclohexadienyl dehydrogenase (EC 1.3.1.12)(EC 1.3.1.43) / 5-Enolpyruvylshikimate-3-phosphate synthase (EC 2.5.1.19), at 2,970,891 to 2,973,098
_2518
Pf6N2E2_2519 - Biosynthetic Aromatic amino acid aminotransferase beta (EC 2.6.1.57), at 2,973,127 to 2,974,239
_2519
Pf6N2E2_2520 - Chorismate mutase I (EC 5.4.99.5) / Prephenate dehydratase (EC 4.2.1.51), at 2,974,253 to 2,975,347
_2520
Position (kb)
2973
2974
2975 Strain fitness (log2 ratio)
-1
0
1 at 2972.225 kb on - strand, within Pf6N2E2_2518 at 2972.225 kb on - strand, within Pf6N2E2_2518 at 2972.355 kb on - strand, within Pf6N2E2_2518 at 2972.355 kb on - strand, within Pf6N2E2_2518 at 2973.099 kb on - strand at 2973.099 kb on - strand at 2973.191 kb on - strand at 2973.191 kb on - strand at 2973.222 kb on - strand at 2973.222 kb on - strand at 2973.222 kb on - strand at 2973.222 kb on - strand at 2973.222 kb on - strand at 2973.226 kb on - strand at 2973.226 kb on - strand at 2973.546 kb on - strand, within Pf6N2E2_2519
Per-strain Table
Position Strand Gene LocusTag Fraction Vancomycin 0.12 mg/ml remove 2,972,225 - Pf6N2E2_2518 0.60 +0.0 2,972,225 - Pf6N2E2_2518 0.60 +1.5 2,972,355 - Pf6N2E2_2518 0.66 -0.9 2,972,355 - Pf6N2E2_2518 0.66 -1.0 2,973,099 - +0.6 2,973,099 - +0.8 2,973,191 - +0.1 2,973,191 - +0.1 2,973,222 - +0.8 2,973,222 - -0.1 2,973,222 - +0.9 2,973,222 - +1.4 2,973,222 - +1.1 2,973,226 - +0.2 2,973,226 - +0.1 2,973,546 - Pf6N2E2_2519 0.38 +1.3
Or see this region's nucleotide sequence