Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_2362

Experiment: Vancomycin 0.12 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_2361 and Pf6N2E2_2362 are separated by 475 nucleotidesPf6N2E2_2362 and Pf6N2E2_2363 overlap by 26 nucleotidesPf6N2E2_2363 and Pf6N2E2_2364 are separated by 4 nucleotides Pf6N2E2_2361 - Type III secretion chaperone protein for YopD (SycD), at 2,796,383 to 2,796,865 _2361 Pf6N2E2_2362 - Type III secretion inner membrane protein (YscU,SpaS,EscU,HrcU,SsaU, homologous to flagellar export components), at 2,797,341 to 2,798,375 _2362 Pf6N2E2_2363 - Type III secretion inner membrane protein (YscT,HrcT,SpaR,EscT,EpaR1,homologous to flagellar export components), at 2,798,350 to 2,799,153 _2363 Pf6N2E2_2364 - Type III secretion inner membrane protein (YscS,homologous to flagellar export components), at 2,799,158 to 2,799,412 _2364 Position (kb) 2797 2798 2799Strain fitness (log2 ratio) -2 -1 0 1 2at 2796.369 kb on + strandat 2796.369 kb on + strandat 2796.369 kb on + strandat 2796.369 kb on + strandat 2796.369 kb on + strandat 2796.369 kb on + strandat 2796.370 kb on - strandat 2796.370 kb on - strandat 2796.370 kb on - strandat 2796.370 kb on - strandat 2796.370 kb on - strandat 2796.370 kb on - strandat 2796.429 kb on + strandat 2796.430 kb on - strandat 2796.430 kb on - strandat 2796.430 kb on - strandat 2796.430 kb on - strandat 2796.480 kb on + strand, within Pf6N2E2_2361at 2796.481 kb on - strand, within Pf6N2E2_2361at 2796.481 kb on - strand, within Pf6N2E2_2361at 2796.481 kb on - strand, within Pf6N2E2_2361at 2796.540 kb on + strand, within Pf6N2E2_2361at 2796.541 kb on - strand, within Pf6N2E2_2361at 2796.589 kb on - strand, within Pf6N2E2_2361at 2796.589 kb on - strand, within Pf6N2E2_2361at 2796.651 kb on + strand, within Pf6N2E2_2361at 2796.663 kb on + strand, within Pf6N2E2_2361at 2796.663 kb on + strand, within Pf6N2E2_2361at 2796.663 kb on + strand, within Pf6N2E2_2361at 2796.663 kb on + strand, within Pf6N2E2_2361at 2796.664 kb on - strand, within Pf6N2E2_2361at 2796.673 kb on - strand, within Pf6N2E2_2361at 2796.726 kb on + strand, within Pf6N2E2_2361at 2796.726 kb on + strand, within Pf6N2E2_2361at 2796.727 kb on - strand, within Pf6N2E2_2361at 2796.727 kb on - strand, within Pf6N2E2_2361at 2796.727 kb on - strand, within Pf6N2E2_2361at 2796.727 kb on - strand, within Pf6N2E2_2361at 2796.747 kb on + strand, within Pf6N2E2_2361at 2797.249 kb on + strandat 2797.417 kb on + strandat 2797.417 kb on + strandat 2797.492 kb on + strand, within Pf6N2E2_2362at 2797.492 kb on + strand, within Pf6N2E2_2362at 2797.492 kb on + strand, within Pf6N2E2_2362at 2797.570 kb on + strand, within Pf6N2E2_2362at 2797.570 kb on + strand, within Pf6N2E2_2362at 2797.766 kb on - strand, within Pf6N2E2_2362at 2797.948 kb on + strand, within Pf6N2E2_2362at 2797.949 kb on - strand, within Pf6N2E2_2362at 2798.207 kb on - strand, within Pf6N2E2_2362at 2798.248 kb on + strand, within Pf6N2E2_2362at 2798.249 kb on - strand, within Pf6N2E2_2362at 2798.465 kb on + strand, within Pf6N2E2_2363at 2798.659 kb on + strand, within Pf6N2E2_2363at 2798.693 kb on + strand, within Pf6N2E2_2363at 2798.745 kb on - strand, within Pf6N2E2_2363at 2798.908 kb on + strand, within Pf6N2E2_2363at 2798.985 kb on - strand, within Pf6N2E2_2363at 2799.125 kb on + strandat 2799.126 kb on - strandat 2799.159 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Vancomycin 0.12 mg/ml
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2,796,369 + -0.4
2,796,369 + +0.4
2,796,369 + +0.6
2,796,369 + +0.6
2,796,369 + -0.2
2,796,369 + -0.1
2,796,370 - -0.9
2,796,370 - +0.8
2,796,370 - -1.0
2,796,370 - -0.8
2,796,370 - +0.1
2,796,370 - +0.1
2,796,429 + +0.9
2,796,430 - +0.1
2,796,430 - -0.9
2,796,430 - +0.4
2,796,430 - +0.1
2,796,480 + Pf6N2E2_2361 0.20 +0.0
2,796,481 - Pf6N2E2_2361 0.20 +1.7
2,796,481 - Pf6N2E2_2361 0.20 +1.6
2,796,481 - Pf6N2E2_2361 0.20 +0.9
2,796,540 + Pf6N2E2_2361 0.33 -0.7
2,796,541 - Pf6N2E2_2361 0.33 +0.6
2,796,589 - Pf6N2E2_2361 0.43 -0.2
2,796,589 - Pf6N2E2_2361 0.43 +0.7
2,796,651 + Pf6N2E2_2361 0.55 +1.0
2,796,663 + Pf6N2E2_2361 0.58 +0.5
2,796,663 + Pf6N2E2_2361 0.58 +0.2
2,796,663 + Pf6N2E2_2361 0.58 -1.0
2,796,663 + Pf6N2E2_2361 0.58 -0.3
2,796,664 - Pf6N2E2_2361 0.58 -0.3
2,796,673 - Pf6N2E2_2361 0.60 +0.0
2,796,726 + Pf6N2E2_2361 0.71 +0.3
2,796,726 + Pf6N2E2_2361 0.71 -0.6
2,796,727 - Pf6N2E2_2361 0.71 -1.9
2,796,727 - Pf6N2E2_2361 0.71 +0.6
2,796,727 - Pf6N2E2_2361 0.71 +0.1
2,796,727 - Pf6N2E2_2361 0.71 +1.3
2,796,747 + Pf6N2E2_2361 0.75 -0.2
2,797,249 + -0.2
2,797,417 + -1.0
2,797,417 + +0.8
2,797,492 + Pf6N2E2_2362 0.15 -0.2
2,797,492 + Pf6N2E2_2362 0.15 -0.0
2,797,492 + Pf6N2E2_2362 0.15 +1.1
2,797,570 + Pf6N2E2_2362 0.22 -0.8
2,797,570 + Pf6N2E2_2362 0.22 +1.8
2,797,766 - Pf6N2E2_2362 0.41 +0.7
2,797,948 + Pf6N2E2_2362 0.59 -0.2
2,797,949 - Pf6N2E2_2362 0.59 +1.0
2,798,207 - Pf6N2E2_2362 0.84 +1.5
2,798,248 + Pf6N2E2_2362 0.88 +1.1
2,798,249 - Pf6N2E2_2362 0.88 +0.4
2,798,465 + Pf6N2E2_2363 0.14 -1.8
2,798,659 + Pf6N2E2_2363 0.38 -0.3
2,798,693 + Pf6N2E2_2363 0.43 -1.1
2,798,745 - Pf6N2E2_2363 0.49 -1.5
2,798,908 + Pf6N2E2_2363 0.69 +0.4
2,798,985 - Pf6N2E2_2363 0.79 +1.7
2,799,125 + +0.5
2,799,126 - +1.4
2,799,159 + -0.4

Or see this region's nucleotide sequence