Experiment: R2A with nitrite 25 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK54_RS13500 and LRK54_RS13505 overlap by 1 nucleotides LRK54_RS13505 and LRK54_RS13510 are separated by 115 nucleotides LRK54_RS13510 and LRK54_RS13515 overlap by 4 nucleotides LRK54_RS13515 and LRK54_RS13520 are separated by 91 nucleotides
LRK54_RS13500: LRK54_RS13500 - flagellar motor switch protein FliG, at 3,024,066 to 3,025,082
_RS13500
LRK54_RS13505: LRK54_RS13505 - flagellar assembly protein FliH, at 3,025,082 to 3,025,702
_RS13505
LRK54_RS13510: LRK54_RS13510 - flagellar protein export ATPase FliI, at 3,025,818 to 3,027,185
_RS13510
LRK54_RS13515: LRK54_RS13515 - flagellar export protein FliJ, at 3,027,182 to 3,027,607
_RS13515
LRK54_RS13520: LRK54_RS13520 - flagellar hook-length control protein FliK, at 3,027,699 to 3,028,799
_RS13520
Position (kb)
3025
3026
3027
3028 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3025.316 kb on + strand, within LRK54_RS13505 at 3025.316 kb on + strand, within LRK54_RS13505 at 3025.316 kb on + strand, within LRK54_RS13505 at 3025.316 kb on + strand, within LRK54_RS13505 at 3025.316 kb on + strand, within LRK54_RS13505 at 3025.317 kb on - strand, within LRK54_RS13505 at 3025.317 kb on - strand, within LRK54_RS13505 at 3025.317 kb on - strand, within LRK54_RS13505 at 3025.317 kb on - strand, within LRK54_RS13505 at 3025.317 kb on - strand, within LRK54_RS13505 at 3025.721 kb on + strand at 3025.721 kb on + strand at 3025.766 kb on + strand at 3025.766 kb on + strand at 3025.766 kb on + strand at 3025.766 kb on + strand at 3025.767 kb on - strand at 3025.767 kb on - strand at 3025.853 kb on + strand at 3025.853 kb on + strand at 3025.853 kb on + strand at 3025.854 kb on - strand at 3026.115 kb on + strand, within LRK54_RS13510 at 3026.397 kb on + strand, within LRK54_RS13510 at 3026.397 kb on + strand, within LRK54_RS13510 at 3026.397 kb on + strand, within LRK54_RS13510 at 3026.398 kb on - strand, within LRK54_RS13510 at 3026.398 kb on - strand, within LRK54_RS13510 at 3026.559 kb on + strand, within LRK54_RS13510 at 3026.559 kb on + strand, within LRK54_RS13510 at 3026.781 kb on + strand, within LRK54_RS13510 at 3026.781 kb on + strand, within LRK54_RS13510 at 3026.781 kb on + strand, within LRK54_RS13510 at 3026.782 kb on - strand, within LRK54_RS13510 at 3026.782 kb on - strand, within LRK54_RS13510 at 3026.782 kb on - strand, within LRK54_RS13510 at 3026.782 kb on - strand, within LRK54_RS13510 at 3026.782 kb on - strand, within LRK54_RS13510 at 3026.782 kb on - strand, within LRK54_RS13510 at 3026.782 kb on - strand, within LRK54_RS13510 at 3026.782 kb on - strand, within LRK54_RS13510 at 3026.782 kb on - strand, within LRK54_RS13510 at 3026.782 kb on - strand, within LRK54_RS13510 at 3026.782 kb on - strand, within LRK54_RS13510 at 3026.782 kb on - strand, within LRK54_RS13510 at 3026.782 kb on - strand, within LRK54_RS13510 at 3026.858 kb on + strand, within LRK54_RS13510 at 3026.858 kb on + strand, within LRK54_RS13510 at 3026.859 kb on - strand, within LRK54_RS13510 at 3026.991 kb on + strand, within LRK54_RS13510 at 3026.991 kb on + strand, within LRK54_RS13510 at 3026.991 kb on + strand, within LRK54_RS13510 at 3026.991 kb on + strand, within LRK54_RS13510 at 3026.991 kb on + strand, within LRK54_RS13510 at 3026.991 kb on + strand, within LRK54_RS13510 at 3026.992 kb on - strand, within LRK54_RS13510 at 3026.992 kb on - strand, within LRK54_RS13510 at 3026.992 kb on - strand, within LRK54_RS13510 at 3027.036 kb on + strand, within LRK54_RS13510 at 3027.037 kb on - strand, within LRK54_RS13510 at 3027.329 kb on + strand, within LRK54_RS13515 at 3027.330 kb on - strand, within LRK54_RS13515 at 3027.590 kb on + strand at 3027.590 kb on + strand at 3027.591 kb on - strand at 3027.591 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with nitrite 25 mM remove 3,025,316 + LRK54_RS13505 0.38 -0.1 3,025,316 + LRK54_RS13505 0.38 +0.3 3,025,316 + LRK54_RS13505 0.38 +0.3 3,025,316 + LRK54_RS13505 0.38 +0.4 3,025,316 + LRK54_RS13505 0.38 +1.2 3,025,317 - LRK54_RS13505 0.38 +0.5 3,025,317 - LRK54_RS13505 0.38 +1.1 3,025,317 - LRK54_RS13505 0.38 +0.2 3,025,317 - LRK54_RS13505 0.38 +0.8 3,025,317 - LRK54_RS13505 0.38 -0.4 3,025,721 + -0.9 3,025,721 + +0.5 3,025,766 + -1.5 3,025,766 + +0.4 3,025,766 + -0.9 3,025,766 + +0.5 3,025,767 - -0.7 3,025,767 - +0.2 3,025,853 + +1.3 3,025,853 + +1.1 3,025,853 + -0.5 3,025,854 - -0.5 3,026,115 + LRK54_RS13510 0.22 -1.3 3,026,397 + LRK54_RS13510 0.42 -0.7 3,026,397 + LRK54_RS13510 0.42 +1.0 3,026,397 + LRK54_RS13510 0.42 -0.4 3,026,398 - LRK54_RS13510 0.42 -1.2 3,026,398 - LRK54_RS13510 0.42 +0.5 3,026,559 + LRK54_RS13510 0.54 -1.1 3,026,559 + LRK54_RS13510 0.54 +0.4 3,026,781 + LRK54_RS13510 0.70 -0.3 3,026,781 + LRK54_RS13510 0.70 +0.7 3,026,781 + LRK54_RS13510 0.70 +0.0 3,026,782 - LRK54_RS13510 0.70 +0.3 3,026,782 - LRK54_RS13510 0.70 -0.6 3,026,782 - LRK54_RS13510 0.70 +1.5 3,026,782 - LRK54_RS13510 0.70 -0.9 3,026,782 - LRK54_RS13510 0.70 +0.4 3,026,782 - LRK54_RS13510 0.70 +0.5 3,026,782 - LRK54_RS13510 0.70 +1.7 3,026,782 - LRK54_RS13510 0.70 +2.1 3,026,782 - LRK54_RS13510 0.70 -1.0 3,026,782 - LRK54_RS13510 0.70 +0.4 3,026,782 - LRK54_RS13510 0.70 -0.2 3,026,782 - LRK54_RS13510 0.70 -0.4 3,026,782 - LRK54_RS13510 0.70 +0.2 3,026,858 + LRK54_RS13510 0.76 -0.3 3,026,858 + LRK54_RS13510 0.76 +0.2 3,026,859 - LRK54_RS13510 0.76 +0.8 3,026,991 + LRK54_RS13510 0.86 +1.2 3,026,991 + LRK54_RS13510 0.86 +0.0 3,026,991 + LRK54_RS13510 0.86 -0.4 3,026,991 + LRK54_RS13510 0.86 +1.3 3,026,991 + LRK54_RS13510 0.86 -0.5 3,026,991 + LRK54_RS13510 0.86 -0.7 3,026,992 - LRK54_RS13510 0.86 -0.3 3,026,992 - LRK54_RS13510 0.86 -2.2 3,026,992 - LRK54_RS13510 0.86 -0.0 3,027,036 + LRK54_RS13510 0.89 +1.7 3,027,037 - LRK54_RS13510 0.89 +0.3 3,027,329 + LRK54_RS13515 0.35 +0.6 3,027,330 - LRK54_RS13515 0.35 +0.6 3,027,590 + +0.8 3,027,590 + +1.0 3,027,591 - -1.2 3,027,591 - +0.3
Or see this region's nucleotide sequence