Experiment: Polymyxin B sulfate 20 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_3328 and Shew_3329 are separated by 250 nucleotides Shew_3329 and Shew_3330 are separated by 176 nucleotides
Shew_3328: Shew_3328 - Ig domain-containing protein (RefSeq), at 3,969,688 to 3,972,324
_3328
Shew_3329: Shew_3329 - diguanylate cyclase/phosphodiesterase with GAF sensor (RefSeq), at 3,972,575 to 3,974,506
_3329
Shew_3330: Shew_3330 - diguanylate cyclase (RefSeq), at 3,974,683 to 3,975,888
_3330
Position (kb)
3972
3973
3974
3975 Strain fitness (log2 ratio)
-2
-1
0
1 at 3971.707 kb on - strand, within Shew_3328 at 3971.836 kb on + strand, within Shew_3328 at 3971.936 kb on - strand, within Shew_3328 at 3971.946 kb on - strand, within Shew_3328 at 3972.289 kb on - strand at 3973.346 kb on - strand, within Shew_3329 at 3973.361 kb on - strand, within Shew_3329 at 3973.538 kb on - strand, within Shew_3329 at 3973.686 kb on - strand, within Shew_3329 at 3973.691 kb on + strand, within Shew_3329 at 3973.699 kb on - strand, within Shew_3329 at 3973.720 kb on + strand, within Shew_3329 at 3973.844 kb on + strand, within Shew_3329 at 3973.959 kb on + strand, within Shew_3329 at 3974.074 kb on + strand, within Shew_3329 at 3974.106 kb on + strand, within Shew_3329 at 3974.145 kb on - strand, within Shew_3329 at 3974.399 kb on + strand at 3974.413 kb on - strand at 3974.440 kb on + strand at 3974.448 kb on - strand at 3974.523 kb on + strand at 3974.531 kb on - strand at 3974.749 kb on - strand at 3974.749 kb on - strand at 3974.768 kb on + strand at 3975.105 kb on - strand, within Shew_3330 at 3975.213 kb on + strand, within Shew_3330 at 3975.454 kb on - strand, within Shew_3330 at 3975.454 kb on - strand, within Shew_3330
Per-strain Table
Position Strand Gene LocusTag Fraction Polymyxin B sulfate 20 mg/ml remove 3,971,707 - Shew_3328 0.77 -0.2 3,971,836 + Shew_3328 0.81 -1.2 3,971,936 - Shew_3328 0.85 +0.2 3,971,946 - Shew_3328 0.86 -0.2 3,972,289 - +0.4 3,973,346 - Shew_3329 0.40 +0.1 3,973,361 - Shew_3329 0.41 +0.2 3,973,538 - Shew_3329 0.50 -0.5 3,973,686 - Shew_3329 0.58 +0.4 3,973,691 + Shew_3329 0.58 -0.1 3,973,699 - Shew_3329 0.58 +0.5 3,973,720 + Shew_3329 0.59 +0.1 3,973,844 + Shew_3329 0.66 -1.1 3,973,959 + Shew_3329 0.72 -0.1 3,974,074 + Shew_3329 0.78 +0.0 3,974,106 + Shew_3329 0.79 -2.1 3,974,145 - Shew_3329 0.81 +0.1 3,974,399 + -0.0 3,974,413 - +0.2 3,974,440 + +0.5 3,974,448 - +0.3 3,974,523 + +0.2 3,974,531 - +0.5 3,974,749 - +1.4 3,974,749 - +0.3 3,974,768 + +0.7 3,975,105 - Shew_3330 0.35 +0.1 3,975,213 + Shew_3330 0.44 -0.1 3,975,454 - Shew_3330 0.64 +0.5 3,975,454 - Shew_3330 0.64 +0.1
Or see this region's nucleotide sequence