Experiment: Polymyxin B sulfate 20 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_3264 and Shew_3265 are separated by 525 nucleotides Shew_3265 and dipZ overlap by 4 nucleotides dipZ and Shew_3267 are separated by 152 nucleotides
Shew_3264: Shew_3264 - FxsA cytoplasmic membrane protein (RefSeq), at 3,902,711 to 3,903,304
_3264
Shew_3265: Shew_3265 - CutA1 divalent ion tolerance protein (RefSeq), at 3,903,830 to 3,904,153
_3265
Shew_3266: dipZ - thiol:disulfide interchange protein precursor (RefSeq), at 3,904,150 to 3,905,958
dipZ
Shew_3267: Shew_3267 - sodium/hydrogen exchanger (RefSeq), at 3,906,111 to 3,907,739
_3267
Position (kb)
3904
3905
3906 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 3903.221 kb on + strand, within Shew_3264 at 3903.421 kb on - strand at 3903.680 kb on - strand at 3903.788 kb on + strand at 3903.956 kb on + strand, within Shew_3265 at 3904.763 kb on - strand, within dipZ at 3905.585 kb on + strand, within dipZ at 3905.751 kb on - strand, within dipZ at 3905.841 kb on - strand at 3906.179 kb on + strand at 3906.269 kb on + strand at 3906.343 kb on - strand, within Shew_3267 at 3906.745 kb on - strand, within Shew_3267 at 3906.847 kb on - strand, within Shew_3267
Per-strain Table
Position Strand Gene LocusTag Fraction Polymyxin B sulfate 20 mg/ml remove 3,903,221 + Shew_3264 0.86 +1.1 3,903,421 - +0.1 3,903,680 - -2.7 3,903,788 + +0.7 3,903,956 + Shew_3265 0.39 -0.2 3,904,763 - dipZ Shew_3266 0.34 -4.2 3,905,585 + dipZ Shew_3266 0.79 -2.8 3,905,751 - dipZ Shew_3266 0.89 -1.2 3,905,841 - -1.8 3,906,179 + +0.2 3,906,269 + +0.6 3,906,343 - Shew_3267 0.14 +0.3 3,906,745 - Shew_3267 0.39 +2.3 3,906,847 - Shew_3267 0.45 +1.2
Or see this region's nucleotide sequence