Experiment: Polymyxin B sulfate 20 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_3081 and Shew_3082 are separated by 615 nucleotides Shew_3082 and Shew_3083 are separated by 181 nucleotides
Shew_3081: Shew_3081 - TonB-dependent receptor (RefSeq), at 3,657,178 to 3,659,751
_3081
Shew_3082: Shew_3082 - sigma-54 dependent trancsriptional regulator (RefSeq), at 3,660,367 to 3,661,815
_3082
Shew_3083: Shew_3083 - 4Fe-4S ferredoxin iron-sulfur binding domain-containing protein (RefSeq), at 3,661,997 to 3,663,439
_3083
Position (kb)
3660
3661
3662 Strain fitness (log2 ratio)
-2
-1
0
1 at 3659.431 kb on + strand, within Shew_3081 at 3659.486 kb on + strand, within Shew_3081 at 3659.494 kb on - strand at 3659.820 kb on + strand at 3660.220 kb on + strand at 3660.432 kb on + strand at 3660.557 kb on + strand, within Shew_3082 at 3660.588 kb on + strand, within Shew_3082 at 3660.793 kb on + strand, within Shew_3082 at 3660.886 kb on - strand, within Shew_3082 at 3660.994 kb on + strand, within Shew_3082 at 3661.002 kb on - strand, within Shew_3082 at 3661.002 kb on - strand, within Shew_3082 at 3661.002 kb on - strand, within Shew_3082 at 3661.050 kb on - strand, within Shew_3082 at 3661.223 kb on + strand, within Shew_3082 at 3661.427 kb on + strand, within Shew_3082 at 3661.533 kb on - strand, within Shew_3082 at 3661.562 kb on + strand, within Shew_3082 at 3661.709 kb on - strand at 3661.777 kb on - strand at 3661.777 kb on - strand at 3662.001 kb on + strand at 3662.418 kb on - strand, within Shew_3083 at 3662.505 kb on - strand, within Shew_3083 at 3662.585 kb on - strand, within Shew_3083 at 3662.729 kb on - strand, within Shew_3083 at 3662.814 kb on + strand, within Shew_3083
Per-strain Table
Position Strand Gene LocusTag Fraction Polymyxin B sulfate 20 mg/ml remove 3,659,431 + Shew_3081 0.88 -0.1 3,659,486 + Shew_3081 0.90 +0.1 3,659,494 - +0.0 3,659,820 + -2.6 3,660,220 + -0.2 3,660,432 + -0.9 3,660,557 + Shew_3082 0.13 +1.7 3,660,588 + Shew_3082 0.15 -0.0 3,660,793 + Shew_3082 0.29 +0.0 3,660,886 - Shew_3082 0.36 -0.6 3,660,994 + Shew_3082 0.43 -1.9 3,661,002 - Shew_3082 0.44 -1.1 3,661,002 - Shew_3082 0.44 -0.3 3,661,002 - Shew_3082 0.44 +0.6 3,661,050 - Shew_3082 0.47 +0.1 3,661,223 + Shew_3082 0.59 +0.1 3,661,427 + Shew_3082 0.73 -1.9 3,661,533 - Shew_3082 0.80 -0.8 3,661,562 + Shew_3082 0.82 -1.0 3,661,709 - +0.1 3,661,777 - -0.0 3,661,777 - +1.7 3,662,001 + +0.8 3,662,418 - Shew_3083 0.29 +0.3 3,662,505 - Shew_3083 0.35 -0.6 3,662,585 - Shew_3083 0.41 -0.6 3,662,729 - Shew_3083 0.51 -0.2 3,662,814 + Shew_3083 0.57 -0.6
Or see this region's nucleotide sequence