Experiment: Polymyxin B sulfate 20 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_3034 and Shew_3035 are separated by 221 nucleotides Shew_3035 and Shew_3036 are separated by 163 nucleotides Shew_3036 and Shew_3037 overlap by 16 nucleotides
Shew_3034: Shew_3034 - diguanylate cyclase (RefSeq), at 3,604,337 to 3,605,905
_3034
Shew_3035: Shew_3035 - hypothetical protein (RefSeq), at 3,606,127 to 3,606,375
_3035
Shew_3036: Shew_3036 - hypothetical protein (RefSeq), at 3,606,539 to 3,607,366
_3036
Shew_3037: Shew_3037 - alpha-L-glutamate ligase (RefSeq), at 3,607,351 to 3,608,304
_3037
Position (kb)
3606
3607
3608 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3605.543 kb on + strand, within Shew_3034 at 3605.923 kb on - strand at 3606.006 kb on - strand at 3606.078 kb on + strand at 3606.086 kb on - strand at 3606.086 kb on - strand at 3606.088 kb on + strand at 3606.170 kb on - strand, within Shew_3035 at 3606.414 kb on - strand at 3606.452 kb on - strand at 3606.752 kb on + strand, within Shew_3036 at 3606.888 kb on + strand, within Shew_3036 at 3606.888 kb on + strand, within Shew_3036 at 3606.896 kb on - strand, within Shew_3036 at 3607.283 kb on - strand, within Shew_3036 at 3607.424 kb on + strand at 3607.424 kb on + strand at 3607.448 kb on - strand, within Shew_3037 at 3607.448 kb on - strand, within Shew_3037 at 3607.931 kb on - strand, within Shew_3037 at 3607.945 kb on + strand, within Shew_3037 at 3607.978 kb on + strand, within Shew_3037 at 3608.003 kb on + strand, within Shew_3037 at 3608.020 kb on - strand, within Shew_3037 at 3608.106 kb on + strand, within Shew_3037 at 3608.268 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Polymyxin B sulfate 20 mg/ml remove 3,605,543 + Shew_3034 0.77 +0.1 3,605,923 - -0.2 3,606,006 - -0.0 3,606,078 + +0.2 3,606,086 - -0.1 3,606,086 - +0.2 3,606,088 + +0.5 3,606,170 - Shew_3035 0.17 -0.1 3,606,414 - -0.2 3,606,452 - -1.3 3,606,752 + Shew_3036 0.26 -1.5 3,606,888 + Shew_3036 0.42 -2.2 3,606,888 + Shew_3036 0.42 +0.3 3,606,896 - Shew_3036 0.43 +0.1 3,607,283 - Shew_3036 0.90 +0.1 3,607,424 + +0.5 3,607,424 + +0.7 3,607,448 - Shew_3037 0.10 -0.5 3,607,448 - Shew_3037 0.10 -3.2 3,607,931 - Shew_3037 0.61 -1.7 3,607,945 + Shew_3037 0.62 +0.2 3,607,978 + Shew_3037 0.66 +0.4 3,608,003 + Shew_3037 0.68 +0.3 3,608,020 - Shew_3037 0.70 -0.1 3,608,106 + Shew_3037 0.79 -1.1 3,608,268 - -1.1
Or see this region's nucleotide sequence