Experiment: Polymyxin B sulfate 20 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_2732 and Shew_2733 are separated by 109 nucleotides Shew_2733 and Shew_2734 are separated by 509 nucleotides Shew_2734 and Shew_2735 are separated by 257 nucleotides
Shew_2732: Shew_2732 - hypothetical protein (RefSeq), at 3,230,428 to 3,230,904
_2732
Shew_2733: Shew_2733 - GCN5-related N-acetyltransferase (RefSeq), at 3,231,014 to 3,231,358
_2733
Shew_2734: Shew_2734 - 4-carboxymuconolactone decarboxylase (RefSeq), at 3,231,868 to 3,232,251
_2734
Shew_2735: Shew_2735 - patatin (RefSeq), at 3,232,509 to 3,233,423
_2735
Position (kb)
3231
3232
3233 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 3230.877 kb on + strand at 3230.881 kb on - strand at 3230.886 kb on - strand at 3230.889 kb on + strand at 3230.897 kb on - strand at 3230.897 kb on - strand at 3230.897 kb on - strand at 3230.898 kb on + strand at 3230.900 kb on + strand at 3230.900 kb on + strand at 3230.900 kb on + strand at 3230.900 kb on + strand at 3230.900 kb on + strand at 3230.900 kb on + strand at 3230.902 kb on + strand at 3230.907 kb on + strand at 3230.908 kb on - strand at 3230.908 kb on - strand at 3230.908 kb on - strand at 3230.908 kb on - strand at 3230.908 kb on - strand at 3230.908 kb on - strand at 3230.908 kb on - strand at 3230.908 kb on - strand at 3230.908 kb on - strand at 3230.908 kb on - strand at 3230.908 kb on - strand at 3230.910 kb on + strand at 3230.910 kb on + strand at 3230.910 kb on + strand at 3230.910 kb on + strand at 3230.910 kb on + strand at 3230.910 kb on + strand at 3230.918 kb on - strand at 3230.918 kb on - strand at 3230.918 kb on - strand at 3230.918 kb on - strand at 3230.918 kb on - strand at 3230.918 kb on - strand at 3230.928 kb on - strand at 3230.929 kb on - strand at 3230.975 kb on + strand at 3230.990 kb on + strand at 3230.999 kb on - strand at 3231.001 kb on + strand at 3231.010 kb on + strand at 3231.010 kb on + strand at 3231.018 kb on - strand at 3231.018 kb on - strand at 3231.018 kb on - strand at 3231.026 kb on - strand at 3231.034 kb on + strand at 3231.034 kb on + strand at 3231.035 kb on + strand at 3231.035 kb on + strand at 3231.056 kb on - strand, within Shew_2733 at 3231.056 kb on - strand, within Shew_2733 at 3231.195 kb on + strand, within Shew_2733 at 3231.199 kb on - strand, within Shew_2733 at 3231.205 kb on + strand, within Shew_2733 at 3231.206 kb on + strand, within Shew_2733 at 3231.206 kb on + strand, within Shew_2733 at 3231.206 kb on + strand, within Shew_2733 at 3231.213 kb on - strand, within Shew_2733 at 3231.214 kb on - strand, within Shew_2733 at 3231.214 kb on - strand, within Shew_2733 at 3231.220 kb on - strand, within Shew_2733 at 3231.220 kb on - strand, within Shew_2733 at 3231.220 kb on - strand, within Shew_2733 at 3231.269 kb on - strand, within Shew_2733 at 3231.275 kb on + strand, within Shew_2733 at 3231.276 kb on + strand, within Shew_2733 at 3231.276 kb on + strand, within Shew_2733 at 3231.276 kb on + strand, within Shew_2733 at 3231.276 kb on + strand, within Shew_2733 at 3231.283 kb on - strand, within Shew_2733 at 3231.302 kb on - strand, within Shew_2733 at 3231.306 kb on + strand, within Shew_2733 at 3231.306 kb on + strand, within Shew_2733 at 3231.306 kb on + strand, within Shew_2733 at 3231.307 kb on + strand, within Shew_2733 at 3231.307 kb on + strand, within Shew_2733 at 3231.307 kb on + strand, within Shew_2733 at 3231.314 kb on - strand, within Shew_2733 at 3231.314 kb on - strand, within Shew_2733 at 3231.314 kb on - strand, within Shew_2733 at 3231.315 kb on - strand, within Shew_2733 at 3231.315 kb on - strand, within Shew_2733 at 3231.315 kb on - strand, within Shew_2733 at 3231.315 kb on - strand, within Shew_2733 at 3231.315 kb on - strand, within Shew_2733 at 3231.325 kb on - strand at 3231.365 kb on + strand at 3231.365 kb on + strand at 3231.365 kb on + strand at 3231.365 kb on + strand at 3231.365 kb on + strand at 3231.365 kb on + strand at 3231.365 kb on + strand at 3231.365 kb on + strand at 3231.370 kb on + strand at 3231.373 kb on - strand at 3231.373 kb on - strand at 3231.373 kb on - strand at 3231.373 kb on - strand at 3231.373 kb on - strand at 3231.373 kb on - strand at 3231.373 kb on - strand at 3231.373 kb on - strand at 3231.380 kb on - strand at 3231.395 kb on + strand at 3231.395 kb on + strand at 3231.395 kb on - strand at 3231.395 kb on + strand at 3231.403 kb on - strand at 3231.409 kb on - strand at 3231.409 kb on - strand at 3231.431 kb on - strand at 3231.436 kb on - strand at 3231.443 kb on - strand at 3231.500 kb on - strand at 3231.513 kb on + strand at 3231.677 kb on - strand at 3231.718 kb on + strand at 3231.799 kb on - strand at 3231.823 kb on - strand at 3231.829 kb on + strand at 3231.868 kb on + strand at 3232.233 kb on - strand at 3232.401 kb on + strand at 3232.401 kb on + strand at 3232.409 kb on - strand at 3232.437 kb on + strand at 3232.437 kb on + strand at 3232.471 kb on + strand at 3232.474 kb on - strand at 3232.474 kb on - strand at 3232.479 kb on - strand at 3232.638 kb on + strand, within Shew_2735 at 3232.768 kb on + strand, within Shew_2735 at 3233.117 kb on - strand, within Shew_2735 at 3233.230 kb on - strand, within Shew_2735
Per-strain Table
Position Strand Gene LocusTag Fraction Polymyxin B sulfate 20 mg/ml remove 3,230,877 + +0.5 3,230,881 - +0.0 3,230,886 - -0.4 3,230,889 + -3.3 3,230,897 - +0.8 3,230,897 - +0.1 3,230,897 - -2.2 3,230,898 + -0.1 3,230,900 + -0.1 3,230,900 + +0.1 3,230,900 + +0.2 3,230,900 + +1.0 3,230,900 + -0.1 3,230,900 + -1.2 3,230,902 + +0.3 3,230,907 + +0.0 3,230,908 - -0.0 3,230,908 - -0.9 3,230,908 - +0.8 3,230,908 - +0.7 3,230,908 - +0.5 3,230,908 - -1.5 3,230,908 - -0.1 3,230,908 - +0.9 3,230,908 - -1.5 3,230,908 - +0.4 3,230,908 - -0.5 3,230,910 + +0.3 3,230,910 + +0.5 3,230,910 + -0.2 3,230,910 + +0.0 3,230,910 + +0.3 3,230,910 + -2.1 3,230,918 - -0.1 3,230,918 - -3.8 3,230,918 - -3.5 3,230,918 - -0.0 3,230,918 - +0.1 3,230,918 - -0.8 3,230,928 - -1.3 3,230,929 - -3.3 3,230,975 + +2.7 3,230,990 + -0.7 3,230,999 - -2.7 3,231,001 + -0.6 3,231,010 + -0.2 3,231,010 + -1.0 3,231,018 - -0.3 3,231,018 - -0.4 3,231,018 - +0.1 3,231,026 - -2.8 3,231,034 + +0.6 3,231,034 + +1.4 3,231,035 + +0.5 3,231,035 + -0.7 3,231,056 - Shew_2733 0.12 +0.1 3,231,056 - Shew_2733 0.12 +0.0 3,231,195 + Shew_2733 0.52 -1.0 3,231,199 - Shew_2733 0.54 -1.5 3,231,205 + Shew_2733 0.55 +0.1 3,231,206 + Shew_2733 0.56 +0.1 3,231,206 + Shew_2733 0.56 -1.7 3,231,206 + Shew_2733 0.56 +0.8 3,231,213 - Shew_2733 0.58 -0.5 3,231,214 - Shew_2733 0.58 +0.1 3,231,214 - Shew_2733 0.58 -0.8 3,231,220 - Shew_2733 0.60 -1.3 3,231,220 - Shew_2733 0.60 +0.4 3,231,220 - Shew_2733 0.60 -0.9 3,231,269 - Shew_2733 0.74 -0.5 3,231,275 + Shew_2733 0.76 +0.2 3,231,276 + Shew_2733 0.76 +0.2 3,231,276 + Shew_2733 0.76 +0.1 3,231,276 + Shew_2733 0.76 -0.0 3,231,276 + Shew_2733 0.76 -0.8 3,231,283 - Shew_2733 0.78 +0.6 3,231,302 - Shew_2733 0.83 -0.4 3,231,306 + Shew_2733 0.85 +0.7 3,231,306 + Shew_2733 0.85 +3.2 3,231,306 + Shew_2733 0.85 -0.2 3,231,307 + Shew_2733 0.85 -0.0 3,231,307 + Shew_2733 0.85 -0.2 3,231,307 + Shew_2733 0.85 +0.3 3,231,314 - Shew_2733 0.87 +1.1 3,231,314 - Shew_2733 0.87 +1.6 3,231,314 - Shew_2733 0.87 -0.2 3,231,315 - Shew_2733 0.87 -0.2 3,231,315 - Shew_2733 0.87 -1.4 3,231,315 - Shew_2733 0.87 -1.5 3,231,315 - Shew_2733 0.87 -0.5 3,231,315 - Shew_2733 0.87 -0.5 3,231,325 - +1.8 3,231,365 + +1.0 3,231,365 + +0.1 3,231,365 + -0.1 3,231,365 + +0.6 3,231,365 + +0.8 3,231,365 + +1.0 3,231,365 + +0.2 3,231,365 + +0.7 3,231,370 + +1.4 3,231,373 - +0.1 3,231,373 - +0.3 3,231,373 - -0.4 3,231,373 - -0.7 3,231,373 - +0.3 3,231,373 - +0.4 3,231,373 - +0.0 3,231,373 - -0.5 3,231,380 - +0.5 3,231,395 + +0.8 3,231,395 + -0.1 3,231,395 - +1.5 3,231,395 + +0.0 3,231,403 - +0.2 3,231,409 - +1.6 3,231,409 - -0.4 3,231,431 - +0.1 3,231,436 - +2.5 3,231,443 - -2.1 3,231,500 - +0.6 3,231,513 + +0.8 3,231,677 - +0.9 3,231,718 + -0.0 3,231,799 - -1.5 3,231,823 - +1.2 3,231,829 + +0.4 3,231,868 + +0.1 3,232,233 - -0.2 3,232,401 + -1.4 3,232,401 + +1.4 3,232,409 - -0.2 3,232,437 + +0.2 3,232,437 + -1.4 3,232,471 + +0.5 3,232,474 - -0.5 3,232,474 - +0.2 3,232,479 - +0.4 3,232,638 + Shew_2735 0.14 +0.5 3,232,768 + Shew_2735 0.28 +0.0 3,233,117 - Shew_2735 0.66 +0.7 3,233,230 - Shew_2735 0.79 +0.9
Or see this region's nucleotide sequence