Experiment: Polymyxin B sulfate 20 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_2650 and Shew_2651 are separated by 148 nucleotides Shew_2651 and Shew_2652 overlap by 1 nucleotides Shew_2652 and Shew_2653 are separated by 44 nucleotides Shew_2653 and queF are separated by 27 nucleotides
Shew_2650: Shew_2650 - hypothetical protein (RefSeq), at 3,124,727 to 3,125,098
_2650
Shew_2651: Shew_2651 - hypothetical protein (RefSeq), at 3,125,247 to 3,125,582
_2651
Shew_2652: Shew_2652 - hypothetical protein (RefSeq), at 3,125,582 to 3,126,355
_2652
Shew_2653: Shew_2653 - SecY interacting protein Syd (RefSeq), at 3,126,400 to 3,127,062
_2653
Shew_2654: queF - 7-cyano-7-deazaguanine reductase (RefSeq), at 3,127,090 to 3,127,980
queF
Position (kb) 3125
3126
3127 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 3124.875 kb on + strand, within Shew_2650 at 3124.883 kb on - strand, within Shew_2650 at 3125.009 kb on - strand, within Shew_2650 at 3125.055 kb on - strand, within Shew_2650 at 3125.055 kb on - strand, within Shew_2650 at 3125.292 kb on - strand, within Shew_2651 at 3125.304 kb on + strand, within Shew_2651 at 3125.326 kb on + strand, within Shew_2651 at 3125.361 kb on - strand, within Shew_2651 at 3125.470 kb on - strand, within Shew_2651 at 3125.876 kb on - strand, within Shew_2652 at 3125.927 kb on - strand, within Shew_2652 at 3126.246 kb on - strand, within Shew_2652 at 3126.875 kb on - strand, within Shew_2653
Per-strain Table
Position Strand Gene LocusTag Fraction Polymyxin B sulfate 20 mg/ml remove 3,124,875 + Shew_2650 0.40 +0.6 3,124,883 - Shew_2650 0.42 -0.0 3,125,009 - Shew_2650 0.76 +0.5 3,125,055 - Shew_2650 0.88 -0.5 3,125,055 - Shew_2650 0.88 -0.5 3,125,292 - Shew_2651 0.13 +0.4 3,125,304 + Shew_2651 0.17 +0.6 3,125,326 + Shew_2651 0.24 +0.5 3,125,361 - Shew_2651 0.34 +0.5 3,125,470 - Shew_2651 0.66 -3.9 3,125,876 - Shew_2652 0.38 +0.9 3,125,927 - Shew_2652 0.45 +1.2 3,126,246 - Shew_2652 0.86 +0.2 3,126,875 - Shew_2653 0.72 +0.3
Or see this region's nucleotide sequence