Experiment: Polymyxin B sulfate 20 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_2604 and Shew_2605 are separated by 881 nucleotides Shew_2605 and Shew_2606 are separated by 9 nucleotides Shew_2606 and Shew_2607 are separated by 63 nucleotides
Shew_2604: Shew_2604 - restriction endonuclease (RefSeq), at 3,069,224 to 3,070,024
_2604
Shew_2605: Shew_2605 - putative phenylacetate-coenzyme A ligase (RefSeq), at 3,070,906 to 3,072,132
_2605
Shew_2606: Shew_2606 - ABC transporter-related protein (RefSeq), at 3,072,142 to 3,072,960
_2606
Shew_2607: Shew_2607 - putative branched-chain amino acid ABC transporter (RefSeq), at 3,073,024 to 3,074,268
_2607
Position (kb)
3070
3071
3072
3073 Strain fitness (log2 ratio)
-2
-1
0
1 at 3069.909 kb on + strand, within Shew_2604 at 3069.924 kb on - strand, within Shew_2604 at 3069.936 kb on - strand, within Shew_2604 at 3069.946 kb on - strand at 3069.977 kb on - strand at 3069.977 kb on - strand at 3070.106 kb on + strand at 3070.341 kb on + strand at 3070.373 kb on + strand at 3070.405 kb on - strand at 3070.439 kb on - strand at 3070.608 kb on - strand at 3071.097 kb on - strand, within Shew_2605 at 3071.121 kb on + strand, within Shew_2605 at 3071.176 kb on - strand, within Shew_2605 at 3071.176 kb on - strand, within Shew_2605 at 3071.182 kb on + strand, within Shew_2605 at 3071.183 kb on - strand, within Shew_2605 at 3071.183 kb on - strand, within Shew_2605 at 3071.507 kb on + strand, within Shew_2605 at 3071.672 kb on - strand, within Shew_2605 at 3071.942 kb on - strand, within Shew_2605 at 3072.152 kb on + strand at 3072.353 kb on + strand, within Shew_2606 at 3072.353 kb on + strand, within Shew_2606 at 3072.361 kb on - strand, within Shew_2606 at 3072.361 kb on - strand, within Shew_2606 at 3072.361 kb on - strand, within Shew_2606 at 3073.006 kb on - strand at 3073.125 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Polymyxin B sulfate 20 mg/ml remove 3,069,909 + Shew_2604 0.86 +0.8 3,069,924 - Shew_2604 0.87 -0.2 3,069,936 - Shew_2604 0.89 +0.1 3,069,946 - -0.4 3,069,977 - +0.1 3,069,977 - -0.7 3,070,106 + -1.9 3,070,341 + -0.1 3,070,373 + +0.1 3,070,405 - +0.3 3,070,439 - -0.0 3,070,608 - +0.5 3,071,097 - Shew_2605 0.16 -1.1 3,071,121 + Shew_2605 0.18 -0.6 3,071,176 - Shew_2605 0.22 +1.0 3,071,176 - Shew_2605 0.22 -0.7 3,071,182 + Shew_2605 0.22 -0.6 3,071,183 - Shew_2605 0.23 -0.3 3,071,183 - Shew_2605 0.23 +0.2 3,071,507 + Shew_2605 0.49 -0.3 3,071,672 - Shew_2605 0.62 +0.6 3,071,942 - Shew_2605 0.84 +0.1 3,072,152 + -0.6 3,072,353 + Shew_2606 0.26 -0.0 3,072,353 + Shew_2606 0.26 +0.2 3,072,361 - Shew_2606 0.27 +0.6 3,072,361 - Shew_2606 0.27 -0.4 3,072,361 - Shew_2606 0.27 -1.1 3,073,006 - +0.6 3,073,125 + +0.7
Or see this region's nucleotide sequence