Experiment: Polymyxin B sulfate 20 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_2593 and Shew_2594 are separated by 108 nucleotides Shew_2594 and Shew_2595 are separated by 147 nucleotides Shew_2595 and Shew_2596 are separated by 508 nucleotides
Shew_2593: Shew_2593 - acyl-CoA synthetase (RefSeq), at 3,059,120 to 3,060,844
_2593
Shew_2594: Shew_2594 - hypothetical protein (RefSeq), at 3,060,953 to 3,061,225
_2594
Shew_2595: Shew_2595 - hypothetical protein (RefSeq), at 3,061,373 to 3,061,678
_2595
Shew_2596: Shew_2596 - prolyl-tRNA synthetase (RefSeq), at 3,062,187 to 3,063,896
_2596
Position (kb)
3061
3062 Strain fitness (log2 ratio)
-2
-1
0
1 at 3060.858 kb on + strand at 3061.016 kb on + strand, within Shew_2594 at 3061.043 kb on + strand, within Shew_2594 at 3061.195 kb on + strand, within Shew_2594 at 3061.323 kb on + strand at 3061.381 kb on + strand at 3061.497 kb on + strand, within Shew_2595 at 3061.507 kb on + strand, within Shew_2595 at 3061.642 kb on - strand, within Shew_2595 at 3061.653 kb on + strand at 3061.661 kb on - strand at 3061.862 kb on + strand at 3061.902 kb on + strand at 3061.915 kb on + strand at 3062.016 kb on - strand at 3062.019 kb on + strand at 3062.065 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Polymyxin B sulfate 20 mg/ml remove 3,060,858 + +1.5 3,061,016 + Shew_2594 0.23 +0.2 3,061,043 + Shew_2594 0.33 +0.3 3,061,195 + Shew_2594 0.89 +0.6 3,061,323 + -0.2 3,061,381 + +0.7 3,061,497 + Shew_2595 0.41 -1.2 3,061,507 + Shew_2595 0.44 +0.7 3,061,642 - Shew_2595 0.88 -0.5 3,061,653 + -1.2 3,061,661 - +0.9 3,061,862 + -0.1 3,061,902 + +0.6 3,061,915 + -0.1 3,062,016 - -1.1 3,062,019 + -1.9 3,062,065 + -1.3
Or see this region's nucleotide sequence