Experiment: Polymyxin B sulfate 20 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_2208 and Shew_2209 are separated by 813 nucleotides Shew_2209 and Shew_2210 are separated by 81 nucleotides
Shew_2208: Shew_2208 - peptidase S8 and S53, subtilisin, kexin, sedolisin (RefSeq), at 2,570,346 to 2,572,163
_2208
Shew_2209: Shew_2209 - peptidase S8 and S53, subtilisin, kexin, sedolisin (RefSeq), at 2,572,977 to 2,576,453
_2209
Shew_2210: Shew_2210 - proprotein convertase, P (RefSeq), at 2,576,535 to 2,577,746
_2210
Position (kb)
2572
2573
2574
2575
2576
2577 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2572.085 kb on + strand at 2572.130 kb on + strand at 2572.649 kb on - strand at 2573.043 kb on + strand at 2573.175 kb on - strand at 2573.289 kb on + strand at 2573.341 kb on + strand, within Shew_2209 at 2573.353 kb on + strand, within Shew_2209 at 2573.529 kb on - strand, within Shew_2209 at 2573.626 kb on - strand, within Shew_2209 at 2573.975 kb on + strand, within Shew_2209 at 2574.178 kb on + strand, within Shew_2209 at 2574.882 kb on + strand, within Shew_2209 at 2574.887 kb on + strand, within Shew_2209 at 2574.893 kb on + strand, within Shew_2209 at 2575.489 kb on - strand, within Shew_2209 at 2576.196 kb on - strand at 2576.246 kb on + strand at 2576.313 kb on - strand at 2576.691 kb on + strand, within Shew_2210 at 2576.699 kb on - strand, within Shew_2210 at 2577.203 kb on + strand, within Shew_2210
Per-strain Table
Position Strand Gene LocusTag Fraction Polymyxin B sulfate 20 mg/ml remove 2,572,085 + +0.2 2,572,130 + +0.4 2,572,649 - +1.1 2,573,043 + -0.3 2,573,175 - +0.8 2,573,289 + +0.5 2,573,341 + Shew_2209 0.10 +0.4 2,573,353 + Shew_2209 0.11 -0.1 2,573,529 - Shew_2209 0.16 -0.8 2,573,626 - Shew_2209 0.19 +2.5 2,573,975 + Shew_2209 0.29 +0.6 2,574,178 + Shew_2209 0.35 +0.6 2,574,882 + Shew_2209 0.55 +0.6 2,574,887 + Shew_2209 0.55 -0.0 2,574,893 + Shew_2209 0.55 +0.4 2,575,489 - Shew_2209 0.72 -0.2 2,576,196 - +0.9 2,576,246 + +0.3 2,576,313 - +0.0 2,576,691 + Shew_2210 0.13 +0.2 2,576,699 - Shew_2210 0.14 +0.9 2,577,203 + Shew_2210 0.55 -1.9
Or see this region's nucleotide sequence