Experiment: Polymyxin B sulfate 20 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_2141 and Shew_2142 are separated by 255 nucleotides Shew_2142 and Shew_2143 are separated by 57 nucleotides Shew_2143 and Shew_2144 are separated by 54 nucleotides
Shew_2141: Shew_2141 - lytic murein transglycosylase (RefSeq), at 2,487,318 to 2,488,505
_2141
Shew_2142: Shew_2142 - hypothetical protein (RefSeq), at 2,488,761 to 2,489,135
_2142
Shew_2143: Shew_2143 - hypothetical protein (RefSeq), at 2,489,193 to 2,489,876
_2143
Shew_2144: Shew_2144 - response regulator receiver modulated CheW protein (RefSeq), at 2,489,931 to 2,490,884
_2144
Position (kb)
2489
2490 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2488.239 kb on - strand, within Shew_2141 at 2488.347 kb on - strand, within Shew_2141 at 2488.489 kb on - strand at 2488.753 kb on - strand at 2488.860 kb on + strand, within Shew_2142 at 2489.364 kb on + strand, within Shew_2143 at 2489.511 kb on - strand, within Shew_2143 at 2489.659 kb on - strand, within Shew_2143 at 2490.124 kb on - strand, within Shew_2144 at 2490.182 kb on - strand, within Shew_2144 at 2490.875 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Polymyxin B sulfate 20 mg/ml remove 2,488,239 - Shew_2141 0.78 -1.3 2,488,347 - Shew_2141 0.87 -1.6 2,488,489 - -2.4 2,488,753 - -0.3 2,488,860 + Shew_2142 0.26 +0.2 2,489,364 + Shew_2143 0.25 +2.7 2,489,511 - Shew_2143 0.46 +0.8 2,489,659 - Shew_2143 0.68 +0.1 2,490,124 - Shew_2144 0.20 -1.1 2,490,182 - Shew_2144 0.26 -2.4 2,490,875 - +0.5
Or see this region's nucleotide sequence