Experiment: Polymyxin B sulfate 20 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_1796 and Shew_1797 overlap by 14 nucleotides Shew_1797 and Shew_1798 overlap by 8 nucleotides Shew_1798 and Shew_1799 are separated by 208 nucleotides Shew_1799 and Shew_1800 are separated by 17 nucleotides
Shew_1796: Shew_1796 - hypothetical protein (RefSeq), at 2,056,472 to 2,059,096
_1796
Shew_1797: Shew_1797 - paraquat-inducible protein A (RefSeq), at 2,059,083 to 2,059,715
_1797
Shew_1798: Shew_1798 - paraquat-inducible protein A (RefSeq), at 2,059,708 to 2,060,370
_1798
Shew_1799: Shew_1799 - YebG family protein (RefSeq), at 2,060,579 to 2,060,875
_1799
Shew_1800: Shew_1800 - putative GAF sensor protein (RefSeq), at 2,060,893 to 2,061,351
_1800
Position (kb)
2059
2060
2061 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 2058.769 kb on - strand, within Shew_1796 at 2058.860 kb on - strand at 2059.127 kb on - strand at 2059.521 kb on - strand, within Shew_1797 at 2060.045 kb on + strand, within Shew_1798 at 2060.165 kb on + strand, within Shew_1798 at 2060.358 kb on + strand at 2060.484 kb on - strand at 2060.484 kb on - strand at 2060.817 kb on - strand, within Shew_1799 at 2061.132 kb on + strand, within Shew_1800 at 2061.140 kb on - strand, within Shew_1800
Per-strain Table
Position Strand Gene LocusTag Fraction Polymyxin B sulfate 20 mg/ml remove 2,058,769 - Shew_1796 0.88 -4.1 2,058,860 - -4.3 2,059,127 - -4.4 2,059,521 - Shew_1797 0.69 -5.2 2,060,045 + Shew_1798 0.51 -1.5 2,060,165 + Shew_1798 0.69 -3.6 2,060,358 + -3.4 2,060,484 - -2.5 2,060,484 - -1.4 2,060,817 - Shew_1799 0.80 +0.8 2,061,132 + Shew_1800 0.52 -0.5 2,061,140 - Shew_1800 0.54 -2.1
Or see this region's nucleotide sequence