Experiment: Polymyxin B sulfate 20 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_1764 and Shew_1765 are separated by 4 nucleotides Shew_1765 and Shew_1766 are separated by 342 nucleotides Shew_1766 and Shew_1767 are separated by 156 nucleotides
Shew_1764: Shew_1764 - nickel-dependent hydrogenase, large subunit (RefSeq), at 2,015,698 to 2,017,404
_1764
Shew_1765: Shew_1765 - hydrogenase (NiFe) small subunit HydA (RefSeq), at 2,017,409 to 2,018,545
_1765
Shew_1766: Shew_1766 - signal transduction protein (RefSeq), at 2,018,888 to 2,019,304
_1766
Shew_1767: Shew_1767 - ATP-dependent helicase HrpA (RefSeq), at 2,019,461 to 2,023,348
_1767
Position (kb)
2017
2018
2019 Strain fitness (log2 ratio)
-1
0
1 at 2016.439 kb on + strand, within Shew_1764 at 2016.459 kb on + strand, within Shew_1764 at 2016.825 kb on - strand, within Shew_1764 at 2016.948 kb on - strand, within Shew_1764 at 2017.071 kb on + strand, within Shew_1764 at 2017.145 kb on + strand, within Shew_1764 at 2017.158 kb on + strand, within Shew_1764 at 2017.168 kb on + strand, within Shew_1764 at 2017.303 kb on + strand at 2017.680 kb on - strand, within Shew_1765 at 2017.936 kb on - strand, within Shew_1765 at 2017.956 kb on + strand, within Shew_1765 at 2017.988 kb on - strand, within Shew_1765 at 2018.451 kb on - strand at 2018.855 kb on - strand at 2018.997 kb on + strand, within Shew_1766 at 2019.083 kb on - strand, within Shew_1766 at 2019.085 kb on - strand, within Shew_1766 at 2019.170 kb on + strand, within Shew_1766 at 2019.217 kb on + strand, within Shew_1766 at 2019.225 kb on - strand, within Shew_1766 at 2019.466 kb on - strand at 2019.537 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Polymyxin B sulfate 20 mg/ml remove 2,016,439 + Shew_1764 0.43 +1.3 2,016,459 + Shew_1764 0.45 +0.3 2,016,825 - Shew_1764 0.66 -0.7 2,016,948 - Shew_1764 0.73 +0.5 2,017,071 + Shew_1764 0.80 +1.3 2,017,145 + Shew_1764 0.85 -0.2 2,017,158 + Shew_1764 0.86 -0.4 2,017,168 + Shew_1764 0.86 +1.7 2,017,303 + +0.2 2,017,680 - Shew_1765 0.24 +0.2 2,017,936 - Shew_1765 0.46 +0.5 2,017,956 + Shew_1765 0.48 +0.7 2,017,988 - Shew_1765 0.51 +0.7 2,018,451 - +0.4 2,018,855 - +0.3 2,018,997 + Shew_1766 0.26 +0.9 2,019,083 - Shew_1766 0.47 -0.4 2,019,085 - Shew_1766 0.47 -0.2 2,019,170 + Shew_1766 0.68 +1.0 2,019,217 + Shew_1766 0.79 +0.9 2,019,225 - Shew_1766 0.81 -0.8 2,019,466 - -0.4 2,019,537 - +0.5
Or see this region's nucleotide sequence