Experiment: Polymyxin B sulfate 20 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_1694 and Shew_1695 are separated by 92 nucleotides Shew_1695 and Shew_1696 are separated by 650 nucleotides
Shew_1694: Shew_1694 - hypothetical protein (RefSeq), at 1,948,145 to 1,948,657
_1694
Shew_1695: Shew_1695 - hypothetical protein (RefSeq), at 1,948,750 to 1,949,097
_1695
Shew_1696: Shew_1696 - hypothetical protein (RefSeq), at 1,949,748 to 1,950,644
_1696
Position (kb) 1948
1949
1950 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 1947.864 kb on - strand at 1947.941 kb on + strand at 1948.121 kb on - strand at 1948.198 kb on + strand, within Shew_1694 at 1948.206 kb on - strand, within Shew_1694 at 1948.251 kb on - strand, within Shew_1694 at 1948.256 kb on - strand, within Shew_1694 at 1948.285 kb on + strand, within Shew_1694 at 1948.293 kb on - strand, within Shew_1694 at 1948.387 kb on - strand, within Shew_1694 at 1948.434 kb on - strand, within Shew_1694 at 1948.544 kb on - strand, within Shew_1694 at 1948.722 kb on - strand at 1948.724 kb on + strand at 1948.724 kb on + strand at 1948.724 kb on + strand at 1948.724 kb on + strand at 1948.724 kb on + strand at 1948.724 kb on + strand at 1948.724 kb on + strand at 1948.724 kb on + strand at 1948.724 kb on + strand at 1948.724 kb on + strand at 1948.724 kb on + strand at 1948.724 kb on + strand at 1948.724 kb on + strand at 1948.724 kb on + strand at 1948.724 kb on + strand at 1948.732 kb on - strand at 1948.732 kb on - strand at 1948.732 kb on - strand at 1948.732 kb on - strand at 1948.732 kb on - strand at 1948.732 kb on - strand at 1948.732 kb on - strand at 1948.732 kb on - strand at 1948.732 kb on - strand at 1948.732 kb on - strand at 1948.732 kb on - strand at 1948.732 kb on - strand at 1948.732 kb on - strand at 1948.732 kb on - strand at 1948.787 kb on + strand, within Shew_1695 at 1948.795 kb on - strand, within Shew_1695 at 1948.795 kb on - strand, within Shew_1695 at 1948.795 kb on - strand, within Shew_1695 at 1948.795 kb on - strand, within Shew_1695 at 1949.031 kb on - strand, within Shew_1695 at 1949.093 kb on + strand at 1949.101 kb on + strand at 1949.101 kb on - strand at 1949.101 kb on - strand at 1949.101 kb on + strand at 1949.163 kb on + strand at 1949.166 kb on - strand at 1949.217 kb on + strand at 1949.217 kb on + strand at 1949.230 kb on - strand at 1949.231 kb on - strand at 1949.316 kb on + strand at 1949.324 kb on - strand at 1949.382 kb on - strand at 1949.518 kb on + strand at 1949.583 kb on + strand at 1949.789 kb on + strand at 1949.888 kb on + strand, within Shew_1696 at 1949.901 kb on - strand, within Shew_1696
Per-strain Table
Position Strand Gene LocusTag Fraction Polymyxin B sulfate 20 mg/ml remove 1,947,864 - +1.3 1,947,941 + +0.4 1,948,121 - +0.2 1,948,198 + Shew_1694 0.10 +1.0 1,948,206 - Shew_1694 0.12 -1.7 1,948,251 - Shew_1694 0.21 -0.4 1,948,256 - Shew_1694 0.22 +1.1 1,948,285 + Shew_1694 0.27 -0.1 1,948,293 - Shew_1694 0.29 +1.0 1,948,387 - Shew_1694 0.47 +1.3 1,948,434 - Shew_1694 0.56 -2.7 1,948,544 - Shew_1694 0.78 +0.4 1,948,722 - +1.5 1,948,724 + +0.9 1,948,724 + +0.3 1,948,724 + +0.1 1,948,724 + -0.1 1,948,724 + -0.2 1,948,724 + +2.1 1,948,724 + +0.2 1,948,724 + +0.6 1,948,724 + -0.1 1,948,724 + -1.1 1,948,724 + +0.5 1,948,724 + +1.3 1,948,724 + -0.1 1,948,724 + +1.4 1,948,724 + -0.2 1,948,732 - -0.5 1,948,732 - -0.2 1,948,732 - -0.4 1,948,732 - -0.1 1,948,732 - +1.3 1,948,732 - +0.5 1,948,732 - +0.6 1,948,732 - +0.0 1,948,732 - +0.4 1,948,732 - +1.2 1,948,732 - -0.6 1,948,732 - +1.2 1,948,732 - +0.1 1,948,732 - +0.2 1,948,787 + Shew_1695 0.11 -1.4 1,948,795 - Shew_1695 0.13 +0.9 1,948,795 - Shew_1695 0.13 -0.7 1,948,795 - Shew_1695 0.13 -1.2 1,948,795 - Shew_1695 0.13 -0.3 1,949,031 - Shew_1695 0.81 -0.8 1,949,093 + -0.0 1,949,101 + +0.3 1,949,101 - +0.2 1,949,101 - -2.8 1,949,101 + +0.1 1,949,163 + +0.7 1,949,166 - -1.3 1,949,217 + -1.7 1,949,217 + -0.7 1,949,230 - -0.7 1,949,231 - -1.3 1,949,316 + -0.5 1,949,324 - -0.7 1,949,382 - -2.3 1,949,518 + -2.1 1,949,583 + +2.1 1,949,789 + +1.8 1,949,888 + Shew_1696 0.16 +0.7 1,949,901 - Shew_1696 0.17 +1.1
Or see this region's nucleotide sequence