Experiment: Polymyxin B sulfate 20 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_1635 and Shew_1636 are separated by 332 nucleotides Shew_1636 and Shew_1637 are separated by 31 nucleotides Shew_1637 and Shew_1638 are separated by 33 nucleotides
Shew_1635: Shew_1635 - diguanylate cyclase (RefSeq), at 1,870,343 to 1,871,698
_1635
Shew_1636: Shew_1636 - hypothetical protein (RefSeq), at 1,872,031 to 1,872,537
_1636
Shew_1637: Shew_1637 - DNA internalization-related competence protein ComEC/Rec2 (RefSeq), at 1,872,569 to 1,874,854
_1637
Shew_1638: Shew_1638 - lipid A ABC exporter, fused ATPase and inner membrane subunits MsbA (RefSeq), at 1,874,888 to 1,876,702
_1638
Position (kb) 1872
1873
1874
1875 Strain fitness (log2 ratio) -1
0
1 at 1873.123 kb on - strand, within Shew_1637 at 1873.389 kb on + strand, within Shew_1637 at 1873.397 kb on - strand, within Shew_1637 at 1873.559 kb on - strand, within Shew_1637 at 1873.982 kb on - strand, within Shew_1637 at 1874.029 kb on + strand, within Shew_1637 at 1874.462 kb on + strand, within Shew_1637
Per-strain Table
Position Strand Gene LocusTag Fraction Polymyxin B sulfate 20 mg/ml remove 1,873,123 - Shew_1637 0.24 +0.7 1,873,389 + Shew_1637 0.36 -0.7 1,873,397 - Shew_1637 0.36 +0.2 1,873,559 - Shew_1637 0.43 +1.2 1,873,982 - Shew_1637 0.62 -0.0 1,874,029 + Shew_1637 0.64 +1.0 1,874,462 + Shew_1637 0.83 +1.4
Or see this region's nucleotide sequence