Experiment: Polymyxin B sulfate 20 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_1533 and Shew_1534 are separated by 178 nucleotides Shew_1534 and Shew_1535 are separated by 33 nucleotides Shew_1535 and Shew_1536 are separated by 88 nucleotides
Shew_1533: Shew_1533 - acetyl-CoA synthetase (RefSeq), at 1,758,093 to 1,760,045
_1533
Shew_1534: Shew_1534 - integral membrane sensor hybrid histidine kinase (RefSeq), at 1,760,224 to 1,763,646
_1534
Shew_1535: Shew_1535 - fumarylacetoacetate (FAA) hydrolase (RefSeq), at 1,763,680 to 1,764,291
_1535
Shew_1536: Shew_1536 - putative chloramphenicol acetyltransferase (RefSeq), at 1,764,380 to 1,765,006
_1536
Position (kb) 1760
1761
1762
1763
1764 Strain fitness (log2 ratio) -1
0
1
2 at 1759.228 kb on + strand, within Shew_1533 at 1759.228 kb on + strand, within Shew_1533 at 1759.228 kb on + strand, within Shew_1533 at 1759.228 kb on + strand, within Shew_1533 at 1759.228 kb on + strand, within Shew_1533 at 1759.228 kb on + strand, within Shew_1533 at 1759.236 kb on - strand, within Shew_1533 at 1759.236 kb on - strand, within Shew_1533 at 1759.236 kb on - strand, within Shew_1533 at 1759.236 kb on - strand, within Shew_1533 at 1759.236 kb on - strand, within Shew_1533 at 1759.236 kb on - strand, within Shew_1533 at 1759.400 kb on + strand, within Shew_1533 at 1759.400 kb on + strand, within Shew_1533 at 1759.415 kb on + strand, within Shew_1533 at 1759.434 kb on - strand, within Shew_1533 at 1759.501 kb on + strand, within Shew_1533 at 1759.509 kb on - strand, within Shew_1533 at 1759.661 kb on + strand, within Shew_1533 at 1759.661 kb on + strand, within Shew_1533 at 1759.705 kb on - strand, within Shew_1533 at 1759.924 kb on - strand at 1760.018 kb on + strand at 1760.436 kb on - strand at 1760.584 kb on - strand, within Shew_1534 at 1760.625 kb on + strand, within Shew_1534 at 1760.633 kb on - strand, within Shew_1534 at 1760.694 kb on - strand, within Shew_1534 at 1761.449 kb on - strand, within Shew_1534 at 1761.593 kb on + strand, within Shew_1534 at 1761.822 kb on - strand, within Shew_1534 at 1762.041 kb on - strand, within Shew_1534 at 1762.415 kb on + strand, within Shew_1534 at 1762.432 kb on + strand, within Shew_1534 at 1762.437 kb on + strand, within Shew_1534 at 1762.445 kb on - strand, within Shew_1534 at 1762.517 kb on - strand, within Shew_1534 at 1762.874 kb on + strand, within Shew_1534 at 1762.897 kb on - strand, within Shew_1534 at 1763.261 kb on - strand, within Shew_1534 at 1763.670 kb on + strand at 1763.718 kb on + strand at 1764.012 kb on + strand, within Shew_1535 at 1764.158 kb on + strand, within Shew_1535 at 1764.213 kb on + strand, within Shew_1535 at 1764.503 kb on - strand, within Shew_1536
Per-strain Table
Position Strand Gene LocusTag Fraction Polymyxin B sulfate 20 mg/ml remove 1,759,228 + Shew_1533 0.58 -0.1 1,759,228 + Shew_1533 0.58 +0.0 1,759,228 + Shew_1533 0.58 +0.4 1,759,228 + Shew_1533 0.58 +0.4 1,759,228 + Shew_1533 0.58 -0.6 1,759,228 + Shew_1533 0.58 +0.3 1,759,236 - Shew_1533 0.59 +0.3 1,759,236 - Shew_1533 0.59 +0.4 1,759,236 - Shew_1533 0.59 +0.3 1,759,236 - Shew_1533 0.59 +0.4 1,759,236 - Shew_1533 0.59 +0.7 1,759,236 - Shew_1533 0.59 -0.1 1,759,400 + Shew_1533 0.67 +0.6 1,759,400 + Shew_1533 0.67 -0.5 1,759,415 + Shew_1533 0.68 -0.0 1,759,434 - Shew_1533 0.69 -1.2 1,759,501 + Shew_1533 0.72 +0.2 1,759,509 - Shew_1533 0.73 -0.1 1,759,661 + Shew_1533 0.80 -0.9 1,759,661 + Shew_1533 0.80 +0.2 1,759,705 - Shew_1533 0.83 +0.1 1,759,924 - +0.5 1,760,018 + +0.6 1,760,436 - +1.0 1,760,584 - Shew_1534 0.11 +0.3 1,760,625 + Shew_1534 0.12 +0.6 1,760,633 - Shew_1534 0.12 +1.1 1,760,694 - Shew_1534 0.14 +0.1 1,761,449 - Shew_1534 0.36 +0.6 1,761,593 + Shew_1534 0.40 -0.0 1,761,822 - Shew_1534 0.47 +2.3 1,762,041 - Shew_1534 0.53 +1.1 1,762,415 + Shew_1534 0.64 +0.6 1,762,432 + Shew_1534 0.65 +1.3 1,762,437 + Shew_1534 0.65 +1.2 1,762,445 - Shew_1534 0.65 -0.0 1,762,517 - Shew_1534 0.67 +0.6 1,762,874 + Shew_1534 0.77 +0.4 1,762,897 - Shew_1534 0.78 +0.4 1,763,261 - Shew_1534 0.89 +0.1 1,763,670 + -1.1 1,763,718 + +0.5 1,764,012 + Shew_1535 0.54 +0.9 1,764,158 + Shew_1535 0.78 -0.1 1,764,213 + Shew_1535 0.87 +1.0 1,764,503 - Shew_1536 0.20 -0.1
Or see this region's nucleotide sequence