Experiment: Polymyxin B sulfate 20 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_1375 and fliP overlap by 4 nucleotides fliP and Shew_1377 are separated by 8 nucleotides Shew_1377 and fliR are separated by 42 nucleotides fliR and flhB overlap by 1 nucleotides
Shew_1375: Shew_1375 - flagellar biosynthesis protein, FliO (RefSeq), at 1,584,774 to 1,585,151
_1375
Shew_1376: fliP - flagellar biosynthesis protein FliP (RefSeq), at 1,585,148 to 1,585,915
fliP
Shew_1377: Shew_1377 - flagellar biosynthetic protein FliQ (RefSeq), at 1,585,924 to 1,586,193
_1377
Shew_1378: fliR - flagellar biosynthesis protein FliR (RefSeq), at 1,586,236 to 1,587,033
fliR
Shew_1379: flhB - flagellar biosynthesis protein FlhB (RefSeq), at 1,587,033 to 1,588,166
flhB
Position (kb) 1585
1586
1587 Strain fitness (log2 ratio) -1
0
1 at 1584.959 kb on + strand, within Shew_1375 at 1585.373 kb on - strand, within fliP at 1585.514 kb on - strand, within fliP at 1585.542 kb on + strand, within fliP at 1585.603 kb on - strand, within fliP at 1587.009 kb on + strand at 1587.064 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Polymyxin B sulfate 20 mg/ml remove 1,584,959 + Shew_1375 0.49 -1.0 1,585,373 - fliP Shew_1376 0.29 -1.5 1,585,514 - fliP Shew_1376 0.48 -0.9 1,585,542 + fliP Shew_1376 0.51 -1.7 1,585,603 - fliP Shew_1376 0.59 -1.1 1,587,009 + +0.1 1,587,064 - -1.2
Or see this region's nucleotide sequence