Experiment: Polymyxin B sulfate 20 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_1312 and Shew_1313 are separated by 142 nucleotides Shew_1313 and Shew_1314 are separated by 213 nucleotides Shew_1314 and Shew_1315 are separated by 139 nucleotides Shew_1315 and Shew_1316 are separated by 32 nucleotides
Shew_1312: Shew_1312 - RND family efflux transporter MFP subunit (RefSeq), at 1,523,812 to 1,524,927
_1312
Shew_1313: Shew_1313 - TetR family transcriptional regulator (RefSeq), at 1,525,070 to 1,525,681
_1313
Shew_1314: Shew_1314 - hypothetical protein (RefSeq), at 1,525,895 to 1,526,809
_1314
Shew_1315: Shew_1315 - hypothetical protein (RefSeq), at 1,526,949 to 1,527,521
_1315
Shew_1316: Shew_1316 - hypothetical protein (RefSeq), at 1,527,554 to 1,528,033
_1316
Position (kb)
1525
1526
1527 Strain fitness (log2 ratio)
-1
0
1
2 at 1525.008 kb on + strand at 1525.082 kb on + strand at 1525.084 kb on - strand at 1525.205 kb on - strand, within Shew_1313 at 1525.282 kb on + strand, within Shew_1313 at 1525.868 kb on - strand at 1525.887 kb on - strand at 1525.979 kb on + strand at 1526.014 kb on - strand, within Shew_1314 at 1526.015 kb on - strand, within Shew_1314 at 1526.053 kb on + strand, within Shew_1314 at 1526.492 kb on - strand, within Shew_1314 at 1526.492 kb on - strand, within Shew_1314 at 1526.566 kb on + strand, within Shew_1314 at 1527.043 kb on + strand, within Shew_1315 at 1527.379 kb on + strand, within Shew_1315
Per-strain Table
Position Strand Gene LocusTag Fraction Polymyxin B sulfate 20 mg/ml remove 1,525,008 + +0.6 1,525,082 + +0.8 1,525,084 - +0.7 1,525,205 - Shew_1313 0.22 +2.5 1,525,282 + Shew_1313 0.35 +0.2 1,525,868 - -1.5 1,525,887 - +0.1 1,525,979 + +0.2 1,526,014 - Shew_1314 0.13 +0.4 1,526,015 - Shew_1314 0.13 +0.9 1,526,053 + Shew_1314 0.17 +0.4 1,526,492 - Shew_1314 0.65 +0.2 1,526,492 - Shew_1314 0.65 +0.4 1,526,566 + Shew_1314 0.73 +0.2 1,527,043 + Shew_1315 0.16 +1.3 1,527,379 + Shew_1315 0.75 +0.4
Or see this region's nucleotide sequence