Experiment: Polymyxin B sulfate 20 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_1311 and Shew_1312 are separated by 3 nucleotides Shew_1312 and Shew_1313 are separated by 142 nucleotides Shew_1313 and Shew_1314 are separated by 213 nucleotides
Shew_1311: Shew_1311 - acriflavin resistance protein (RefSeq), at 1,520,737 to 1,523,808
_1311
Shew_1312: Shew_1312 - RND family efflux transporter MFP subunit (RefSeq), at 1,523,812 to 1,524,927
_1312
Shew_1313: Shew_1313 - TetR family transcriptional regulator (RefSeq), at 1,525,070 to 1,525,681
_1313
Shew_1314: Shew_1314 - hypothetical protein (RefSeq), at 1,525,895 to 1,526,809
_1314
Position (kb)
1523
1524
1525 Strain fitness (log2 ratio)
-1
0
1
2 at 1522.833 kb on - strand, within Shew_1311 at 1523.014 kb on - strand, within Shew_1311 at 1523.045 kb on + strand, within Shew_1311 at 1523.121 kb on - strand, within Shew_1311 at 1523.871 kb on + strand at 1523.872 kb on + strand at 1523.971 kb on + strand, within Shew_1312 at 1524.097 kb on - strand, within Shew_1312 at 1524.582 kb on - strand, within Shew_1312 at 1524.870 kb on + strand at 1524.870 kb on + strand at 1525.008 kb on + strand at 1525.082 kb on + strand at 1525.084 kb on - strand at 1525.205 kb on - strand, within Shew_1313 at 1525.282 kb on + strand, within Shew_1313 at 1525.868 kb on - strand at 1525.887 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Polymyxin B sulfate 20 mg/ml remove 1,522,833 - Shew_1311 0.68 +0.3 1,523,014 - Shew_1311 0.74 +0.6 1,523,045 + Shew_1311 0.75 +1.3 1,523,121 - Shew_1311 0.78 +0.3 1,523,871 + +0.8 1,523,872 + +0.7 1,523,971 + Shew_1312 0.14 +0.3 1,524,097 - Shew_1312 0.26 +0.6 1,524,582 - Shew_1312 0.69 +0.5 1,524,870 + +1.2 1,524,870 + +0.5 1,525,008 + +0.6 1,525,082 + +0.8 1,525,084 - +0.7 1,525,205 - Shew_1313 0.22 +2.5 1,525,282 + Shew_1313 0.35 +0.2 1,525,868 - -1.5 1,525,887 - +0.1
Or see this region's nucleotide sequence