Experiment: Polymyxin B sulfate 20 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_1171 and Shew_1172 overlap by 8 nucleotides Shew_1172 and Shew_1173 are separated by 43 nucleotides Shew_1173 and Shew_1174 are separated by 193 nucleotides
Shew_1171: Shew_1171 - glucose-1-phosphate adenylyltransferase (RefSeq), at 1,353,301 to 1,354,575
_1171
Shew_1172: Shew_1172 - starch synthase (RefSeq), at 1,354,568 to 1,356,217
_1172
Shew_1173: Shew_1173 - hypothetical protein (RefSeq), at 1,356,261 to 1,356,734
_1173
Shew_1174: Shew_1174 - sulfotransferase (RefSeq), at 1,356,928 to 1,358,523
_1174
Position (kb)
1354
1355
1356
1357 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1353.677 kb on + strand, within Shew_1171 at 1354.126 kb on - strand, within Shew_1171 at 1354.320 kb on - strand, within Shew_1171 at 1354.340 kb on - strand, within Shew_1171 at 1354.355 kb on - strand, within Shew_1171 at 1354.391 kb on + strand, within Shew_1171 at 1354.501 kb on - strand at 1354.502 kb on + strand at 1354.761 kb on - strand, within Shew_1172 at 1354.952 kb on + strand, within Shew_1172 at 1355.040 kb on - strand, within Shew_1172 at 1355.143 kb on - strand, within Shew_1172 at 1355.165 kb on + strand, within Shew_1172 at 1355.273 kb on + strand, within Shew_1172 at 1355.291 kb on + strand, within Shew_1172 at 1355.485 kb on + strand, within Shew_1172 at 1355.509 kb on - strand, within Shew_1172 at 1355.570 kb on - strand, within Shew_1172 at 1355.788 kb on + strand, within Shew_1172 at 1355.841 kb on + strand, within Shew_1172 at 1355.889 kb on + strand, within Shew_1172 at 1355.972 kb on + strand, within Shew_1172 at 1355.972 kb on + strand, within Shew_1172 at 1356.187 kb on + strand at 1356.210 kb on - strand at 1356.211 kb on + strand at 1356.220 kb on - strand at 1356.278 kb on - strand at 1356.294 kb on - strand at 1356.600 kb on + strand, within Shew_1173 at 1356.608 kb on - strand, within Shew_1173 at 1356.629 kb on + strand, within Shew_1173 at 1356.756 kb on + strand at 1356.927 kb on - strand at 1357.096 kb on + strand, within Shew_1174
Per-strain Table
Position Strand Gene LocusTag Fraction Polymyxin B sulfate 20 mg/ml remove 1,353,677 + Shew_1171 0.29 +0.2 1,354,126 - Shew_1171 0.65 +0.2 1,354,320 - Shew_1171 0.80 -0.1 1,354,340 - Shew_1171 0.81 -0.8 1,354,355 - Shew_1171 0.83 -1.3 1,354,391 + Shew_1171 0.85 -0.4 1,354,501 - -0.2 1,354,502 + -0.7 1,354,761 - Shew_1172 0.12 +0.2 1,354,952 + Shew_1172 0.23 +0.3 1,355,040 - Shew_1172 0.29 +1.4 1,355,143 - Shew_1172 0.35 +1.2 1,355,165 + Shew_1172 0.36 -0.1 1,355,273 + Shew_1172 0.43 +0.5 1,355,291 + Shew_1172 0.44 +1.3 1,355,485 + Shew_1172 0.56 +2.4 1,355,509 - Shew_1172 0.57 -0.3 1,355,570 - Shew_1172 0.61 +1.0 1,355,788 + Shew_1172 0.74 +0.6 1,355,841 + Shew_1172 0.77 +1.1 1,355,889 + Shew_1172 0.80 -1.0 1,355,972 + Shew_1172 0.85 -0.3 1,355,972 + Shew_1172 0.85 +0.5 1,356,187 + -0.4 1,356,210 - -1.1 1,356,211 + -0.4 1,356,220 - +1.4 1,356,278 - +0.3 1,356,294 - +0.2 1,356,600 + Shew_1173 0.72 -0.4 1,356,608 - Shew_1173 0.73 -0.2 1,356,629 + Shew_1173 0.78 -0.1 1,356,756 + -0.5 1,356,927 - -2.8 1,357,096 + Shew_1174 0.11 -0.1
Or see this region's nucleotide sequence