Experiment: Polymyxin B sulfate 20 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_1141 and Shew_1142 are separated by 573 nucleotides
Shew_1141: Shew_1141 - transposase IS116/IS110/IS902 family protein (RefSeq), at 1,316,043 to 1,317,056
_1141
Shew_1142: Shew_1142 - putative transcriptional regulator (RefSeq), at 1,317,630 to 1,319,018
_1142
Position (kb)
1317
1318
1319
1320 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1317.312 kb on + strand at 1317.312 kb on + strand at 1317.312 kb on + strand at 1317.312 kb on + strand at 1317.312 kb on + strand at 1317.312 kb on + strand at 1317.312 kb on + strand at 1317.312 kb on + strand at 1317.320 kb on - strand at 1317.320 kb on - strand at 1317.400 kb on - strand at 1317.494 kb on + strand at 1317.542 kb on + strand at 1317.542 kb on + strand at 1317.619 kb on - strand at 1317.715 kb on + strand at 1317.723 kb on - strand at 1317.818 kb on + strand, within Shew_1142 at 1317.819 kb on + strand, within Shew_1142 at 1317.826 kb on - strand, within Shew_1142 at 1317.827 kb on - strand, within Shew_1142 at 1317.842 kb on - strand, within Shew_1142 at 1317.878 kb on + strand, within Shew_1142 at 1317.885 kb on - strand, within Shew_1142 at 1318.042 kb on + strand, within Shew_1142 at 1318.069 kb on + strand, within Shew_1142 at 1318.077 kb on - strand, within Shew_1142 at 1318.077 kb on - strand, within Shew_1142 at 1318.077 kb on - strand, within Shew_1142 at 1318.092 kb on + strand, within Shew_1142 at 1318.100 kb on - strand, within Shew_1142 at 1318.143 kb on + strand, within Shew_1142 at 1318.144 kb on + strand, within Shew_1142 at 1318.152 kb on - strand, within Shew_1142 at 1318.210 kb on + strand, within Shew_1142 at 1318.251 kb on - strand, within Shew_1142 at 1318.268 kb on + strand, within Shew_1142 at 1318.268 kb on + strand, within Shew_1142 at 1318.278 kb on + strand, within Shew_1142 at 1318.304 kb on + strand, within Shew_1142 at 1318.527 kb on + strand, within Shew_1142 at 1318.581 kb on + strand, within Shew_1142 at 1318.589 kb on - strand, within Shew_1142 at 1318.622 kb on - strand, within Shew_1142 at 1318.625 kb on + strand, within Shew_1142 at 1318.633 kb on - strand, within Shew_1142 at 1318.633 kb on - strand, within Shew_1142 at 1318.652 kb on + strand, within Shew_1142 at 1318.660 kb on - strand, within Shew_1142 at 1318.679 kb on - strand, within Shew_1142 at 1318.704 kb on + strand, within Shew_1142 at 1318.721 kb on - strand, within Shew_1142 at 1318.858 kb on + strand, within Shew_1142 at 1318.895 kb on + strand at 1318.903 kb on - strand at 1318.903 kb on - strand at 1318.989 kb on + strand at 1318.997 kb on - strand at 1319.006 kb on + strand at 1319.014 kb on - strand at 1319.044 kb on + strand at 1319.049 kb on + strand at 1319.052 kb on - strand at 1319.052 kb on - strand at 1319.052 kb on - strand at 1319.052 kb on - strand at 1319.117 kb on - strand at 1319.128 kb on + strand at 1319.128 kb on + strand at 1319.128 kb on + strand at 1319.179 kb on + strand at 1319.194 kb on - strand at 1319.194 kb on - strand at 1319.194 kb on - strand at 1319.194 kb on - strand at 1319.194 kb on - strand at 1319.245 kb on + strand at 1319.261 kb on + strand at 1319.261 kb on + strand at 1319.338 kb on - strand at 1319.356 kb on + strand at 1319.356 kb on + strand at 1319.420 kb on + strand at 1319.420 kb on + strand at 1319.428 kb on - strand at 1319.742 kb on - strand at 1319.742 kb on - strand at 1319.855 kb on + strand at 1319.858 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Polymyxin B sulfate 20 mg/ml remove 1,317,312 + -0.7 1,317,312 + +0.2 1,317,312 + +0.3 1,317,312 + -0.5 1,317,312 + +0.3 1,317,312 + +1.5 1,317,312 + -0.2 1,317,312 + +1.0 1,317,320 - +0.2 1,317,320 - -0.2 1,317,400 - -1.0 1,317,494 + -1.1 1,317,542 + -0.6 1,317,542 + +0.4 1,317,619 - +0.1 1,317,715 + +0.3 1,317,723 - +0.9 1,317,818 + Shew_1142 0.14 -0.3 1,317,819 + Shew_1142 0.14 -0.1 1,317,826 - Shew_1142 0.14 +0.3 1,317,827 - Shew_1142 0.14 -0.7 1,317,842 - Shew_1142 0.15 +0.6 1,317,878 + Shew_1142 0.18 +0.2 1,317,885 - Shew_1142 0.18 -0.5 1,318,042 + Shew_1142 0.30 +0.3 1,318,069 + Shew_1142 0.32 -0.1 1,318,077 - Shew_1142 0.32 +0.8 1,318,077 - Shew_1142 0.32 -1.5 1,318,077 - Shew_1142 0.32 -3.3 1,318,092 + Shew_1142 0.33 -1.7 1,318,100 - Shew_1142 0.34 +0.0 1,318,143 + Shew_1142 0.37 +0.0 1,318,144 + Shew_1142 0.37 -0.8 1,318,152 - Shew_1142 0.38 -0.6 1,318,210 + Shew_1142 0.42 -0.3 1,318,251 - Shew_1142 0.45 -0.6 1,318,268 + Shew_1142 0.46 +0.1 1,318,268 + Shew_1142 0.46 +0.1 1,318,278 + Shew_1142 0.47 +0.3 1,318,304 + Shew_1142 0.49 +0.2 1,318,527 + Shew_1142 0.65 +0.5 1,318,581 + Shew_1142 0.68 -0.5 1,318,589 - Shew_1142 0.69 -0.0 1,318,622 - Shew_1142 0.71 +0.2 1,318,625 + Shew_1142 0.72 -1.0 1,318,633 - Shew_1142 0.72 +2.4 1,318,633 - Shew_1142 0.72 -0.0 1,318,652 + Shew_1142 0.74 -0.2 1,318,660 - Shew_1142 0.74 -1.2 1,318,679 - Shew_1142 0.76 +0.3 1,318,704 + Shew_1142 0.77 +0.8 1,318,721 - Shew_1142 0.79 +1.5 1,318,858 + Shew_1142 0.88 +0.7 1,318,895 + +0.1 1,318,903 - +0.6 1,318,903 - +1.5 1,318,989 + -0.4 1,318,997 - -0.1 1,319,006 + +0.9 1,319,014 - -0.1 1,319,044 + +0.3 1,319,049 + +0.9 1,319,052 - -1.1 1,319,052 - -0.0 1,319,052 - +0.2 1,319,052 - -0.1 1,319,117 - +0.9 1,319,128 + -1.9 1,319,128 + +0.4 1,319,128 + +0.7 1,319,179 + +1.1 1,319,194 - +0.1 1,319,194 - -0.5 1,319,194 - -0.1 1,319,194 - -3.2 1,319,194 - -0.5 1,319,245 + +0.5 1,319,261 + +0.2 1,319,261 + -0.1 1,319,338 - -0.8 1,319,356 + -0.2 1,319,356 + -0.2 1,319,420 + +0.2 1,319,420 + +2.5 1,319,428 - +0.0 1,319,742 - -0.5 1,319,742 - +0.8 1,319,855 + +0.5 1,319,858 - +0.5
Or see this region's nucleotide sequence