Experiment: Polymyxin B sulfate 20 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_1099 and Shew_1100 are separated by 9 nucleotides Shew_1100 and Shew_1101 are separated by 3 nucleotides Shew_1101 and Shew_1102 are separated by 2 nucleotides Shew_1102 and Shew_1103 are separated by 231 nucleotides Shew_1103 and Shew_1104 are separated by 0 nucleotides
Shew_1099: Shew_1099 - isoleucyl-tRNA synthetase (RefSeq), at 1,267,071 to 1,269,893
_1099
Shew_1100: Shew_1100 - lipoprotein signal peptidase (RefSeq), at 1,269,903 to 1,270,415
_1100
Shew_1101: Shew_1101 - peptidylprolyl isomerase, FKBP-type (RefSeq), at 1,270,419 to 1,270,841
_1101
Shew_1102: Shew_1102 - hydroxymethylbutenyl pyrophosphate reductase (RefSeq), at 1,270,844 to 1,271,794
_1102
Shew_1103: Shew_1103 - type IV pilus modification protein PilV (RefSeq), at 1,272,026 to 1,272,547
_1103
Shew_1104: Shew_1104 - prepilin-type cleavage/methylation-like protein (RefSeq), at 1,272,548 to 1,273,534
_1104
Position (kb) 1270
1271
1272 Strain fitness (log2 ratio) -1
0
1
2 at 1272.054 kb on + strand at 1272.238 kb on + strand, within Shew_1103 at 1272.284 kb on + strand, within Shew_1103 at 1272.284 kb on + strand, within Shew_1103 at 1272.284 kb on + strand, within Shew_1103 at 1272.284 kb on + strand, within Shew_1103 at 1272.284 kb on + strand, within Shew_1103 at 1272.284 kb on + strand, within Shew_1103 at 1272.284 kb on + strand, within Shew_1103 at 1272.292 kb on - strand, within Shew_1103 at 1272.292 kb on - strand, within Shew_1103 at 1272.292 kb on - strand, within Shew_1103 at 1272.292 kb on - strand, within Shew_1103 at 1272.292 kb on - strand, within Shew_1103 at 1272.292 kb on - strand, within Shew_1103 at 1272.292 kb on - strand, within Shew_1103 at 1272.292 kb on - strand, within Shew_1103 at 1272.453 kb on + strand, within Shew_1103 at 1272.758 kb on - strand, within Shew_1104 at 1272.762 kb on + strand, within Shew_1104
Per-strain Table
Position Strand Gene LocusTag Fraction Polymyxin B sulfate 20 mg/ml remove 1,272,054 + +0.8 1,272,238 + Shew_1103 0.41 +1.3 1,272,284 + Shew_1103 0.49 +1.1 1,272,284 + Shew_1103 0.49 +0.3 1,272,284 + Shew_1103 0.49 +1.1 1,272,284 + Shew_1103 0.49 -0.5 1,272,284 + Shew_1103 0.49 +0.1 1,272,284 + Shew_1103 0.49 +0.3 1,272,284 + Shew_1103 0.49 +0.2 1,272,292 - Shew_1103 0.51 +1.3 1,272,292 - Shew_1103 0.51 +0.0 1,272,292 - Shew_1103 0.51 +1.0 1,272,292 - Shew_1103 0.51 +0.3 1,272,292 - Shew_1103 0.51 -0.3 1,272,292 - Shew_1103 0.51 +2.5 1,272,292 - Shew_1103 0.51 +0.1 1,272,292 - Shew_1103 0.51 +0.1 1,272,453 + Shew_1103 0.82 +0.9 1,272,758 - Shew_1104 0.21 -1.0 1,272,762 + Shew_1104 0.22 -0.0
Or see this region's nucleotide sequence