Experiment: Polymyxin B sulfate 20 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_1056 and Shew_1057 are separated by 128 nucleotides Shew_1057 and Shew_1058 are separated by 44 nucleotides Shew_1058 and Shew_1059 are separated by 266 nucleotides Shew_1059 and Shew_1060 are separated by 66 nucleotides
Shew_1056: Shew_1056 - DNA repair protein RecO (RefSeq), at 1,218,707 to 1,219,402
_1056
Shew_1057: Shew_1057 - pyridoxal phosphate biosynthetic protein PdxJ (RefSeq), at 1,219,531 to 1,220,268
_1057
Shew_1058: Shew_1058 - holo-acyl-carrier-protein synthase (RefSeq), at 1,220,313 to 1,220,714
_1058
Shew_1059: Shew_1059 - hypothetical protein (RefSeq), at 1,220,981 to 1,221,523
_1059
Shew_1060: Shew_1060 - hypothetical protein (RefSeq), at 1,221,590 to 1,222,873
_1060
Position (kb)
1220
1221 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1219.380 kb on - strand at 1220.302 kb on + strand at 1220.419 kb on - strand, within Shew_1058 at 1220.421 kb on + strand, within Shew_1058 at 1220.576 kb on + strand, within Shew_1058 at 1220.644 kb on + strand, within Shew_1058 at 1221.033 kb on - strand at 1221.293 kb on - strand, within Shew_1059 at 1221.431 kb on - strand, within Shew_1059 at 1221.491 kb on + strand at 1221.602 kb on + strand at 1221.635 kb on - strand at 1221.673 kb on + strand at 1221.713 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Polymyxin B sulfate 20 mg/ml remove 1,219,380 - +0.6 1,220,302 + +0.8 1,220,419 - Shew_1058 0.26 -3.6 1,220,421 + Shew_1058 0.27 -3.0 1,220,576 + Shew_1058 0.65 -1.1 1,220,644 + Shew_1058 0.82 -0.5 1,221,033 - +0.7 1,221,293 - Shew_1059 0.57 +0.6 1,221,431 - Shew_1059 0.83 +0.6 1,221,491 + +0.1 1,221,602 + -1.3 1,221,635 - +0.3 1,221,673 + -0.1 1,221,713 - -1.1
Or see this region's nucleotide sequence