Experiment: Polymyxin B sulfate 20 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_0887 and Shew_0888 are separated by 117 nucleotides Shew_0888 and Shew_0889 are separated by 267 nucleotides Shew_0889 and Shew_0890 are separated by 1 nucleotides
Shew_0887: Shew_0887 - D-isomer specific 2-hydroxyacid dehydrogenase, NAD-binding (RefSeq), at 1,007,257 to 1,008,210
_0887
Shew_0888: Shew_0888 - no description, at 1,008,328 to 1,009,505
_0888
Shew_0889: Shew_0889 - hypothetical protein (RefSeq), at 1,009,773 to 1,010,162
_0889
Shew_0890: Shew_0890 - secretion protein HlyD family protein (RefSeq), at 1,010,164 to 1,011,300
_0890
Position (kb)
1008
1009
1010 Strain fitness (log2 ratio)
-1
0
1 at 1007.376 kb on - strand, within Shew_0887 at 1007.386 kb on - strand, within Shew_0887 at 1007.496 kb on + strand, within Shew_0887 at 1007.504 kb on - strand, within Shew_0887 at 1007.504 kb on - strand, within Shew_0887 at 1007.830 kb on - strand, within Shew_0887 at 1007.919 kb on + strand, within Shew_0887 at 1008.095 kb on + strand, within Shew_0887 at 1008.165 kb on + strand at 1008.173 kb on - strand at 1008.246 kb on - strand at 1008.403 kb on + strand at 1008.603 kb on + strand, within Shew_0888 at 1008.667 kb on + strand, within Shew_0888 at 1009.014 kb on + strand, within Shew_0888 at 1009.064 kb on + strand, within Shew_0888 at 1009.158 kb on + strand, within Shew_0888 at 1009.619 kb on - strand at 1009.935 kb on - strand, within Shew_0889 at 1010.060 kb on + strand, within Shew_0889 at 1010.505 kb on + strand, within Shew_0890
Per-strain Table
Position Strand Gene LocusTag Fraction Polymyxin B sulfate 20 mg/ml remove 1,007,376 - Shew_0887 0.12 +0.0 1,007,386 - Shew_0887 0.14 +0.6 1,007,496 + Shew_0887 0.25 +0.8 1,007,504 - Shew_0887 0.26 +0.6 1,007,504 - Shew_0887 0.26 +0.5 1,007,830 - Shew_0887 0.60 -0.3 1,007,919 + Shew_0887 0.69 +0.6 1,008,095 + Shew_0887 0.88 +0.2 1,008,165 + +0.6 1,008,173 - +0.2 1,008,246 - +0.2 1,008,403 + +0.4 1,008,603 + Shew_0888 0.23 +0.6 1,008,667 + Shew_0888 0.29 -0.5 1,009,014 + Shew_0888 0.58 +1.0 1,009,064 + Shew_0888 0.62 +0.9 1,009,158 + Shew_0888 0.70 +1.2 1,009,619 - -0.3 1,009,935 - Shew_0889 0.42 +0.5 1,010,060 + Shew_0889 0.74 +0.2 1,010,505 + Shew_0890 0.30 +0.8
Or see this region's nucleotide sequence