Experiment: Polymyxin B sulfate 20 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_0874 and Shew_0875 are separated by 86 nucleotides Shew_0875 and Shew_0876 are separated by 103 nucleotides Shew_0876 and apaH are separated by 104 nucleotides apaH and apaG are separated by 26 nucleotides
Shew_0874: Shew_0874 - dihydrofolate reductase (RefSeq), at 993,823 to 994,305
_0874
Shew_0875: Shew_0875 - hypothetical protein (RefSeq), at 994,392 to 994,784
_0875
Shew_0876: Shew_0876 - methyl-accepting chemotaxis sensory transducer (RefSeq), at 994,888 to 996,912
_0876
Shew_0877: apaH - diadenosine tetraphosphatase (RefSeq), at 997,017 to 997,841
apaH
Shew_0878: apaG - ApaG (RefSeq), at 997,868 to 998,248
apaG
Position (kb) 994
995
996
997 Strain fitness (log2 ratio) -1
0
1 at 994.458 kb on + strand, within Shew_0875 at 994.847 kb on + strand at 995.131 kb on - strand, within Shew_0876 at 995.705 kb on + strand, within Shew_0876 at 995.708 kb on - strand, within Shew_0876 at 996.115 kb on - strand, within Shew_0876 at 996.167 kb on + strand, within Shew_0876 at 996.312 kb on + strand, within Shew_0876 at 996.312 kb on + strand, within Shew_0876 at 997.140 kb on + strand, within apaH at 997.320 kb on - strand, within apaH at 997.448 kb on - strand, within apaH at 997.458 kb on - strand, within apaH at 997.615 kb on - strand, within apaH
Per-strain Table
Position Strand Gene LocusTag Fraction Polymyxin B sulfate 20 mg/ml remove 994,458 + Shew_0875 0.17 -0.4 994,847 + -1.4 995,131 - Shew_0876 0.12 +0.6 995,705 + Shew_0876 0.40 -0.4 995,708 - Shew_0876 0.40 -0.7 996,115 - Shew_0876 0.61 +0.2 996,167 + Shew_0876 0.63 -0.6 996,312 + Shew_0876 0.70 -0.8 996,312 + Shew_0876 0.70 -0.1 997,140 + apaH Shew_0877 0.15 +1.1 997,320 - apaH Shew_0877 0.37 +0.6 997,448 - apaH Shew_0877 0.52 +0.5 997,458 - apaH Shew_0877 0.53 -0.4 997,615 - apaH Shew_0877 0.72 +0.1
Or see this region's nucleotide sequence