Experiment: Polymyxin B sulfate 20 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_0582 and Shew_0583 overlap by 4 nucleotides Shew_0583 and Shew_0584 are separated by 110 nucleotides Shew_0584 and Shew_0585 are separated by 81 nucleotides
Shew_0582: Shew_0582 - integral membrane sensor signal transduction histidine kinase (RefSeq), at 660,077 to 661,414
_0582
Shew_0583: Shew_0583 - two component transcriptional regulator (RefSeq), at 661,411 to 662,085
_0583
Shew_0584: Shew_0584 - hypothetical protein (RefSeq), at 662,196 to 662,534
_0584
Shew_0585: Shew_0585 - cAMP-regulatory protein (RefSeq), at 662,616 to 663,251
_0585
Position (kb) 661
662
663 Strain fitness (log2 ratio) -2
-1
0
1
2 at 660.474 kb on - strand, within Shew_0582 at 660.761 kb on + strand, within Shew_0582 at 660.820 kb on + strand, within Shew_0582 at 661.022 kb on + strand, within Shew_0582 at 661.093 kb on - strand, within Shew_0582 at 661.216 kb on + strand, within Shew_0582 at 661.438 kb on + strand at 661.474 kb on - strand at 661.685 kb on - strand, within Shew_0583 at 661.685 kb on - strand, within Shew_0583 at 662.378 kb on + strand, within Shew_0584
Per-strain Table
Position Strand Gene LocusTag Fraction Polymyxin B sulfate 20 mg/ml remove 660,474 - Shew_0582 0.30 -0.8 660,761 + Shew_0582 0.51 -0.7 660,820 + Shew_0582 0.56 +0.2 661,022 + Shew_0582 0.71 +0.3 661,093 - Shew_0582 0.76 +1.3 661,216 + Shew_0582 0.85 -2.6 661,438 + -0.0 661,474 - -0.8 661,685 - Shew_0583 0.41 +2.6 661,685 - Shew_0583 0.41 +0.4 662,378 + Shew_0584 0.54 +0.6
Or see this region's nucleotide sequence