Experiment: Polymyxin B sulfate 20 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_0467 and Shew_0468 are separated by 196 nucleotides Shew_0468 and Shew_0469 are separated by 2 nucleotides Shew_0469 and Shew_0470 are separated by 16 nucleotides
Shew_0467: Shew_0467 - RND family efflux transporter MFP subunit (RefSeq), at 524,597 to 525,679
_0467
Shew_0468: Shew_0468 - LysR family transcriptional regulator (RefSeq), at 525,876 to 526,820
_0468
Shew_0469: Shew_0469 - pentapeptide repeat-containing protein (RefSeq), at 526,823 to 527,494
_0469
Shew_0470: Shew_0470 - MATE efflux family protein (RefSeq), at 527,511 to 528,878
_0470
Position (kb)
525
526
527 Strain fitness (log2 ratio)
-1
0
1
2 at 524.994 kb on + strand, within Shew_0467 at 525.179 kb on - strand, within Shew_0467 at 525.214 kb on + strand, within Shew_0467 at 525.214 kb on + strand, within Shew_0467 at 525.233 kb on - strand, within Shew_0467 at 525.255 kb on - strand, within Shew_0467 at 525.579 kb on - strand at 525.897 kb on - strand at 525.907 kb on - strand at 526.044 kb on - strand, within Shew_0468 at 526.106 kb on + strand, within Shew_0468 at 526.192 kb on - strand, within Shew_0468 at 526.549 kb on + strand, within Shew_0468 at 526.557 kb on - strand, within Shew_0468 at 527.035 kb on + strand, within Shew_0469 at 527.573 kb on + strand at 527.763 kb on + strand, within Shew_0470
Per-strain Table
Position Strand Gene LocusTag Fraction Polymyxin B sulfate 20 mg/ml remove 524,994 + Shew_0467 0.37 +0.8 525,179 - Shew_0467 0.54 +0.6 525,214 + Shew_0467 0.57 +0.7 525,214 + Shew_0467 0.57 +1.0 525,233 - Shew_0467 0.59 +1.7 525,255 - Shew_0467 0.61 +0.3 525,579 - +1.0 525,897 - +2.0 525,907 - +0.7 526,044 - Shew_0468 0.18 +1.0 526,106 + Shew_0468 0.24 +0.5 526,192 - Shew_0468 0.33 +0.7 526,549 + Shew_0468 0.71 +0.8 526,557 - Shew_0468 0.72 +1.2 527,035 + Shew_0469 0.32 -0.3 527,573 + +0.3 527,763 + Shew_0470 0.18 -0.4
Or see this region's nucleotide sequence