Experiment: Polymyxin B sulfate 20 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_0306 and Shew_R0014 are separated by 218 nucleotides Shew_R0014 and Shew_R0015 are separated by 43 nucleotides Shew_R0015 and Shew_R0016 are separated by 84 nucleotides Shew_R0016 and Shew_R0017 are separated by 52 nucleotides Shew_R0017 and Shew_0307 are separated by 132 nucleotides Shew_0307 and Shew_0308 are separated by 8 nucleotides Shew_0308 and Shew_0309 are separated by 16 nucleotides
Shew_0306: Shew_0306 - diguanylate cyclase/phosphodiesterase with GAF sensor (RefSeq), at 329,116 to 331,764
_0306
Shew_R0014: Shew_R0014 - tRNA-Arg (RefSeq), at 331,983 to 332,059
_R0014
Shew_R0015: Shew_R0015 - tRNA-His (RefSeq), at 332,103 to 332,178
_R0015
Shew_R0016: Shew_R0016 - tRNA-Pro (RefSeq), at 332,263 to 332,339
_R0016
Shew_R0017: Shew_R0017 - tRNA-Pro (RefSeq), at 332,392 to 332,468
_R0017
Shew_0307: Shew_0307 - diguanylate cyclase/phosphodiesterase (RefSeq), at 332,601 to 334,523
_0307
Shew_0308: Shew_0308 - hypothetical protein (RefSeq), at 334,532 to 335,239
_0308
Shew_0309: Shew_0309 - OmpA/MotB domain-containing protein (RefSeq), at 335,256 to 335,861
_0309
Position (kb) 332
333
334
335 Strain fitness (log2 ratio) -2
-1
0
1 at 331.986 kb on + strand at 332.850 kb on - strand, within Shew_0307 at 333.130 kb on - strand, within Shew_0307 at 333.311 kb on + strand, within Shew_0307 at 333.319 kb on + strand, within Shew_0307 at 333.409 kb on - strand, within Shew_0307 at 333.447 kb on + strand, within Shew_0307 at 333.753 kb on + strand, within Shew_0307 at 333.838 kb on + strand, within Shew_0307 at 334.028 kb on + strand, within Shew_0307 at 334.074 kb on + strand, within Shew_0307 at 334.405 kb on + strand at 334.409 kb on + strand at 334.498 kb on + strand at 334.669 kb on + strand, within Shew_0308 at 334.783 kb on - strand, within Shew_0308 at 334.911 kb on - strand, within Shew_0308 at 334.940 kb on - strand, within Shew_0308 at 334.940 kb on - strand, within Shew_0308 at 334.974 kb on + strand, within Shew_0308 at 335.049 kb on + strand, within Shew_0308 at 335.058 kb on - strand, within Shew_0308 at 335.173 kb on - strand at 335.173 kb on - strand at 335.394 kb on + strand, within Shew_0309
Per-strain Table
Position Strand Gene LocusTag Fraction Polymyxin B sulfate 20 mg/ml remove 331,986 + -2.3 332,850 - Shew_0307 0.13 +1.0 333,130 - Shew_0307 0.28 +0.4 333,311 + Shew_0307 0.37 +0.8 333,319 + Shew_0307 0.37 +0.1 333,409 - Shew_0307 0.42 -1.1 333,447 + Shew_0307 0.44 +0.7 333,753 + Shew_0307 0.60 +0.4 333,838 + Shew_0307 0.64 +0.2 334,028 + Shew_0307 0.74 -1.2 334,074 + Shew_0307 0.77 +1.1 334,405 + +0.4 334,409 + +0.3 334,498 + +0.3 334,669 + Shew_0308 0.19 -0.3 334,783 - Shew_0308 0.35 -0.7 334,911 - Shew_0308 0.54 +0.2 334,940 - Shew_0308 0.58 +0.0 334,940 - Shew_0308 0.58 -0.0 334,974 + Shew_0308 0.62 -0.6 335,049 + Shew_0308 0.73 +0.4 335,058 - Shew_0308 0.74 +0.3 335,173 - +0.0 335,173 - -0.3 335,394 + Shew_0309 0.23 +0.0
Or see this region's nucleotide sequence