Experiment: Polymyxin B sulfate 20 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_0077 and Shew_0078 are separated by 136 nucleotides Shew_0078 and Shew_0079 are separated by 203 nucleotides
Shew_0077: Shew_0077 - GCN5-related N-acetyltransferase (RefSeq), at 89,699 to 90,652
_0077
Shew_0078: Shew_0078 - ribokinase-like domain-containing protein (RefSeq), at 90,789 to 91,649
_0078
Shew_0079: Shew_0079 - TAP domain-containing protein (RefSeq), at 91,853 to 93,310
_0079
Position (kb)
90
91
92 Strain fitness (log2 ratio)
-1
0
1 at 89.840 kb on - strand, within Shew_0077 at 89.909 kb on + strand, within Shew_0077 at 89.975 kb on + strand, within Shew_0077 at 90.001 kb on + strand, within Shew_0077 at 90.009 kb on - strand, within Shew_0077 at 90.107 kb on + strand, within Shew_0077 at 90.107 kb on + strand, within Shew_0077 at 90.115 kb on - strand, within Shew_0077 at 90.115 kb on - strand, within Shew_0077 at 90.198 kb on + strand, within Shew_0077 at 90.441 kb on + strand, within Shew_0077 at 90.496 kb on - strand, within Shew_0077 at 90.500 kb on + strand, within Shew_0077 at 90.707 kb on - strand at 90.789 kb on - strand at 90.849 kb on + strand at 90.921 kb on + strand, within Shew_0078 at 90.927 kb on + strand, within Shew_0078 at 90.932 kb on + strand, within Shew_0078 at 90.940 kb on - strand, within Shew_0078 at 90.940 kb on - strand, within Shew_0078 at 90.944 kb on + strand, within Shew_0078 at 90.962 kb on - strand, within Shew_0078 at 91.033 kb on + strand, within Shew_0078 at 91.216 kb on - strand, within Shew_0078 at 91.226 kb on - strand, within Shew_0078 at 91.461 kb on - strand, within Shew_0078 at 91.572 kb on - strand at 91.825 kb on + strand at 91.831 kb on + strand at 91.833 kb on - strand at 91.901 kb on - strand at 91.989 kb on + strand at 91.997 kb on - strand at 92.016 kb on - strand, within Shew_0079 at 92.152 kb on + strand, within Shew_0079 at 92.248 kb on - strand, within Shew_0079 at 92.248 kb on - strand, within Shew_0079 at 92.351 kb on + strand, within Shew_0079 at 92.573 kb on - strand, within Shew_0079 at 92.632 kb on + strand, within Shew_0079
Per-strain Table
Position Strand Gene LocusTag Fraction Polymyxin B sulfate 20 mg/ml remove 89,840 - Shew_0077 0.15 +0.7 89,909 + Shew_0077 0.22 +0.4 89,975 + Shew_0077 0.29 +0.1 90,001 + Shew_0077 0.32 +0.2 90,009 - Shew_0077 0.32 +0.7 90,107 + Shew_0077 0.43 +0.4 90,107 + Shew_0077 0.43 +0.1 90,115 - Shew_0077 0.44 +0.7 90,115 - Shew_0077 0.44 +0.3 90,198 + Shew_0077 0.52 +0.4 90,441 + Shew_0077 0.78 +0.6 90,496 - Shew_0077 0.84 -1.1 90,500 + Shew_0077 0.84 +0.3 90,707 - +1.1 90,789 - +1.2 90,849 + +0.4 90,921 + Shew_0078 0.15 +0.8 90,927 + Shew_0078 0.16 +0.5 90,932 + Shew_0078 0.17 +0.2 90,940 - Shew_0078 0.18 +0.7 90,940 - Shew_0078 0.18 +0.5 90,944 + Shew_0078 0.18 +0.5 90,962 - Shew_0078 0.20 -0.2 91,033 + Shew_0078 0.28 +0.5 91,216 - Shew_0078 0.50 +0.7 91,226 - Shew_0078 0.51 +0.9 91,461 - Shew_0078 0.78 +0.2 91,572 - +0.8 91,825 + +0.2 91,831 + -0.0 91,833 - -0.0 91,901 - +0.6 91,989 + -0.1 91,997 - -0.1 92,016 - Shew_0079 0.11 +0.3 92,152 + Shew_0079 0.21 -0.2 92,248 - Shew_0079 0.27 +0.2 92,248 - Shew_0079 0.27 -0.3 92,351 + Shew_0079 0.34 -0.9 92,573 - Shew_0079 0.49 +0.8 92,632 + Shew_0079 0.53 +0.2
Or see this region's nucleotide sequence