Experiment: Polymyxin B sulfate 20 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_0069 and Shew_0070 are separated by 178 nucleotides Shew_0070 and Shew_0071 are separated by 204 nucleotides
Shew_0069: Shew_0069 - hypothetical protein (RefSeq), at 82,400 to 83,503
_0069
Shew_0070: Shew_0070 - iron-containing alcohol dehydrogenase (RefSeq), at 83,682 to 84,845
_0070
Shew_0071: Shew_0071 - AraC family transcriptional regulator (RefSeq), at 85,050 to 85,916
_0071
Position (kb) 83
84
85 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 82.853 kb on - strand, within Shew_0069 at 82.881 kb on - strand, within Shew_0069 at 83.228 kb on + strand, within Shew_0069 at 83.235 kb on - strand, within Shew_0069 at 83.235 kb on - strand, within Shew_0069 at 83.309 kb on - strand, within Shew_0069 at 83.438 kb on - strand at 83.453 kb on + strand at 83.749 kb on + strand at 83.931 kb on - strand, within Shew_0070 at 83.946 kb on + strand, within Shew_0070 at 83.972 kb on - strand, within Shew_0070 at 84.106 kb on + strand, within Shew_0070 at 84.204 kb on + strand, within Shew_0070 at 84.270 kb on - strand, within Shew_0070 at 84.282 kb on - strand, within Shew_0070 at 84.519 kb on - strand, within Shew_0070 at 84.540 kb on - strand, within Shew_0070 at 84.624 kb on - strand, within Shew_0070 at 84.688 kb on + strand, within Shew_0070 at 84.742 kb on - strand at 84.795 kb on - strand at 84.933 kb on - strand at 85.084 kb on + strand at 85.118 kb on - strand at 85.120 kb on + strand at 85.259 kb on + strand, within Shew_0071 at 85.267 kb on - strand, within Shew_0071 at 85.286 kb on + strand, within Shew_0071 at 85.385 kb on + strand, within Shew_0071 at 85.398 kb on - strand, within Shew_0071 at 85.460 kb on + strand, within Shew_0071 at 85.562 kb on + strand, within Shew_0071 at 85.570 kb on - strand, within Shew_0071 at 85.570 kb on - strand, within Shew_0071 at 85.585 kb on - strand, within Shew_0071 at 85.660 kb on + strand, within Shew_0071 at 85.696 kb on - strand, within Shew_0071 at 85.720 kb on + strand, within Shew_0071 at 85.728 kb on - strand, within Shew_0071 at 85.801 kb on + strand, within Shew_0071
Per-strain Table
Position Strand Gene LocusTag Fraction Polymyxin B sulfate 20 mg/ml remove 82,853 - Shew_0069 0.41 +0.2 82,881 - Shew_0069 0.44 -0.1 83,228 + Shew_0069 0.75 -0.5 83,235 - Shew_0069 0.76 +0.6 83,235 - Shew_0069 0.76 +0.3 83,309 - Shew_0069 0.82 -3.4 83,438 - +0.4 83,453 + +0.7 83,749 + +0.6 83,931 - Shew_0070 0.21 +0.4 83,946 + Shew_0070 0.23 +0.8 83,972 - Shew_0070 0.25 +0.1 84,106 + Shew_0070 0.36 +0.8 84,204 + Shew_0070 0.45 -3.5 84,270 - Shew_0070 0.51 +0.1 84,282 - Shew_0070 0.52 -0.1 84,519 - Shew_0070 0.72 +0.3 84,540 - Shew_0070 0.74 -0.1 84,624 - Shew_0070 0.81 -0.4 84,688 + Shew_0070 0.86 -0.8 84,742 - +1.2 84,795 - +0.0 84,933 - +0.4 85,084 + -0.2 85,118 - +1.0 85,120 + -0.7 85,259 + Shew_0071 0.24 +0.5 85,267 - Shew_0071 0.25 -0.7 85,286 + Shew_0071 0.27 +0.5 85,385 + Shew_0071 0.39 +0.6 85,398 - Shew_0071 0.40 -0.1 85,460 + Shew_0071 0.47 -0.9 85,562 + Shew_0071 0.59 +0.6 85,570 - Shew_0071 0.60 -0.3 85,570 - Shew_0071 0.60 +0.9 85,585 - Shew_0071 0.62 -0.0 85,660 + Shew_0071 0.70 +0.3 85,696 - Shew_0071 0.75 -0.3 85,720 + Shew_0071 0.77 +0.4 85,728 - Shew_0071 0.78 -0.7 85,801 + Shew_0071 0.87 +0.3
Or see this region's nucleotide sequence