Experiment: Polymyxin B sulfate 20 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_0066 and Shew_0067 are separated by 1 nucleotides Shew_0067 and Shew_0068 are separated by 214 nucleotides Shew_0068 and Shew_0069 are separated by 44 nucleotides
Shew_0066: Shew_0066 - hypothetical protein (RefSeq), at 79,885 to 80,250
_0066
Shew_0067: Shew_0067 - hypothetical protein (RefSeq), at 80,252 to 80,761
_0067
Shew_0068: Shew_0068 - fumarate lyase (RefSeq), at 80,976 to 82,355
_0068
Shew_0069: Shew_0069 - hypothetical protein (RefSeq), at 82,400 to 83,503
_0069
Position (kb) 80
81
82
83 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 80.040 kb on + strand, within Shew_0066 at 80.040 kb on + strand, within Shew_0066 at 80.048 kb on - strand, within Shew_0066 at 80.110 kb on + strand, within Shew_0066 at 80.118 kb on - strand, within Shew_0066 at 80.118 kb on - strand, within Shew_0066 at 80.209 kb on - strand, within Shew_0066 at 80.806 kb on + strand at 80.914 kb on - strand at 81.325 kb on + strand, within Shew_0068 at 81.361 kb on + strand, within Shew_0068 at 81.361 kb on + strand, within Shew_0068 at 81.399 kb on + strand, within Shew_0068 at 81.399 kb on + strand, within Shew_0068 at 81.407 kb on - strand, within Shew_0068 at 81.690 kb on - strand, within Shew_0068 at 81.797 kb on + strand, within Shew_0068 at 81.871 kb on + strand, within Shew_0068 at 81.888 kb on - strand, within Shew_0068 at 81.937 kb on - strand, within Shew_0068 at 82.068 kb on - strand, within Shew_0068 at 82.195 kb on + strand, within Shew_0068 at 82.386 kb on + strand at 82.389 kb on - strand at 82.413 kb on + strand at 82.416 kb on - strand at 82.471 kb on + strand at 82.471 kb on + strand at 82.671 kb on - strand, within Shew_0069 at 82.671 kb on - strand, within Shew_0069 at 82.853 kb on - strand, within Shew_0069 at 82.881 kb on - strand, within Shew_0069 at 83.228 kb on + strand, within Shew_0069 at 83.235 kb on - strand, within Shew_0069 at 83.235 kb on - strand, within Shew_0069 at 83.309 kb on - strand, within Shew_0069
Per-strain Table
Position Strand Gene LocusTag Fraction Polymyxin B sulfate 20 mg/ml remove 80,040 + Shew_0066 0.42 +0.1 80,040 + Shew_0066 0.42 +0.2 80,048 - Shew_0066 0.45 -0.1 80,110 + Shew_0066 0.61 -0.9 80,118 - Shew_0066 0.64 -1.3 80,118 - Shew_0066 0.64 +0.6 80,209 - Shew_0066 0.89 +0.1 80,806 + +0.8 80,914 - +0.1 81,325 + Shew_0068 0.25 -0.1 81,361 + Shew_0068 0.28 +0.4 81,361 + Shew_0068 0.28 -0.6 81,399 + Shew_0068 0.31 +0.1 81,399 + Shew_0068 0.31 +0.4 81,407 - Shew_0068 0.31 -0.0 81,690 - Shew_0068 0.52 +0.2 81,797 + Shew_0068 0.59 +3.8 81,871 + Shew_0068 0.65 -0.8 81,888 - Shew_0068 0.66 +0.1 81,937 - Shew_0068 0.70 +0.1 82,068 - Shew_0068 0.79 +0.1 82,195 + Shew_0068 0.88 -0.1 82,386 + +0.4 82,389 - -0.1 82,413 + -0.1 82,416 - +1.6 82,471 + +0.4 82,471 + -2.3 82,671 - Shew_0069 0.25 -0.6 82,671 - Shew_0069 0.25 -0.1 82,853 - Shew_0069 0.41 +0.2 82,881 - Shew_0069 0.44 -0.1 83,228 + Shew_0069 0.75 -0.5 83,235 - Shew_0069 0.76 +0.6 83,235 - Shew_0069 0.76 +0.3 83,309 - Shew_0069 0.82 -3.4
Or see this region's nucleotide sequence