Experiment: Polymyxin B sulfate 20 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_0065 and Shew_0066 are separated by 274 nucleotides Shew_0066 and Shew_0067 are separated by 1 nucleotides Shew_0067 and Shew_0068 are separated by 214 nucleotides
Shew_0065: Shew_0065 - formate dehydrogenase, gamma subunit (RefSeq), at 78,618 to 79,610
_0065
Shew_0066: Shew_0066 - hypothetical protein (RefSeq), at 79,885 to 80,250
_0066
Shew_0067: Shew_0067 - hypothetical protein (RefSeq), at 80,252 to 80,761
_0067
Shew_0068: Shew_0068 - fumarate lyase (RefSeq), at 80,976 to 82,355
_0068
Position (kb) 79
80
81 Strain fitness (log2 ratio) -1
0
1 at 79.008 kb on - strand, within Shew_0065 at 79.008 kb on - strand, within Shew_0065 at 79.038 kb on + strand, within Shew_0065 at 79.106 kb on + strand, within Shew_0065 at 79.114 kb on + strand, within Shew_0065 at 79.259 kb on + strand, within Shew_0065 at 79.267 kb on - strand, within Shew_0065 at 79.414 kb on + strand, within Shew_0065 at 79.538 kb on - strand at 79.717 kb on - strand at 79.744 kb on + strand at 79.758 kb on - strand at 80.040 kb on + strand, within Shew_0066 at 80.040 kb on + strand, within Shew_0066 at 80.048 kb on - strand, within Shew_0066 at 80.110 kb on + strand, within Shew_0066 at 80.118 kb on - strand, within Shew_0066 at 80.118 kb on - strand, within Shew_0066 at 80.209 kb on - strand, within Shew_0066 at 80.806 kb on + strand at 80.914 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Polymyxin B sulfate 20 mg/ml remove 79,008 - Shew_0065 0.39 -0.3 79,008 - Shew_0065 0.39 -1.1 79,038 + Shew_0065 0.42 +1.1 79,106 + Shew_0065 0.49 +1.2 79,114 + Shew_0065 0.50 +0.4 79,259 + Shew_0065 0.65 +0.2 79,267 - Shew_0065 0.65 +0.3 79,414 + Shew_0065 0.80 +0.5 79,538 - +0.8 79,717 - +0.2 79,744 + -0.2 79,758 - +0.4 80,040 + Shew_0066 0.42 +0.1 80,040 + Shew_0066 0.42 +0.2 80,048 - Shew_0066 0.45 -0.1 80,110 + Shew_0066 0.61 -0.9 80,118 - Shew_0066 0.64 -1.3 80,118 - Shew_0066 0.64 +0.6 80,209 - Shew_0066 0.89 +0.1 80,806 + +0.8 80,914 - +0.1
Or see this region's nucleotide sequence