Experiment: Polymyxin B sulfate 20 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_0052 and Shew_0053 are separated by 23 nucleotides Shew_0053 and Shew_0054 are separated by 295 nucleotides Shew_0054 and Shew_0055 overlap by 1 nucleotides
Shew_0052: Shew_0052 - DNA binding domain-containing protein (RefSeq), at 63,788 to 64,693
_0052
Shew_0053: Shew_0053 - formate dehydrogenase, subunit FdhD (RefSeq), at 64,717 to 65,562
_0053
Shew_0054: Shew_0054 - hypothetical protein (RefSeq), at 65,858 to 66,328
_0054
Shew_0055: Shew_0055 - hypothetical protein (RefSeq), at 66,328 to 66,918
_0055
Position (kb) 64
65
66 Strain fitness (log2 ratio) -1
0
1 at 63.806 kb on - strand at 63.834 kb on + strand at 63.836 kb on + strand at 64.081 kb on + strand, within Shew_0052 at 64.089 kb on - strand, within Shew_0052 at 64.089 kb on - strand, within Shew_0052 at 64.094 kb on - strand, within Shew_0052 at 64.284 kb on + strand, within Shew_0052 at 64.292 kb on - strand, within Shew_0052 at 64.292 kb on - strand, within Shew_0052 at 64.326 kb on + strand, within Shew_0052 at 64.334 kb on + strand, within Shew_0052 at 64.611 kb on + strand at 64.619 kb on - strand at 64.673 kb on - strand at 64.701 kb on + strand at 64.836 kb on + strand, within Shew_0053 at 64.844 kb on - strand, within Shew_0053 at 64.919 kb on - strand, within Shew_0053 at 64.972 kb on - strand, within Shew_0053 at 64.990 kb on + strand, within Shew_0053 at 64.998 kb on - strand, within Shew_0053 at 65.037 kb on - strand, within Shew_0053 at 65.053 kb on - strand, within Shew_0053 at 65.053 kb on - strand, within Shew_0053 at 65.055 kb on + strand, within Shew_0053 at 65.055 kb on + strand, within Shew_0053 at 65.055 kb on + strand, within Shew_0053 at 65.055 kb on + strand, within Shew_0053 at 65.055 kb on + strand, within Shew_0053 at 65.062 kb on + strand, within Shew_0053 at 65.063 kb on - strand, within Shew_0053 at 65.063 kb on - strand, within Shew_0053 at 65.063 kb on - strand, within Shew_0053 at 65.070 kb on - strand, within Shew_0053 at 65.199 kb on - strand, within Shew_0053 at 65.394 kb on + strand, within Shew_0053 at 65.528 kb on + strand at 65.536 kb on - strand at 65.594 kb on + strand at 65.720 kb on - strand at 65.991 kb on + strand, within Shew_0054 at 66.021 kb on - strand, within Shew_0054 at 66.063 kb on - strand, within Shew_0054 at 66.178 kb on - strand, within Shew_0054 at 66.258 kb on + strand, within Shew_0054 at 66.291 kb on - strand at 66.362 kb on + strand at 66.486 kb on + strand, within Shew_0055 at 66.545 kb on + strand, within Shew_0055 at 66.553 kb on - strand, within Shew_0055
Per-strain Table
Position Strand Gene LocusTag Fraction Polymyxin B sulfate 20 mg/ml remove 63,806 - +0.1 63,834 + -0.0 63,836 + -1.1 64,081 + Shew_0052 0.32 -0.3 64,089 - Shew_0052 0.33 -0.1 64,089 - Shew_0052 0.33 -1.3 64,094 - Shew_0052 0.34 -0.2 64,284 + Shew_0052 0.55 +0.1 64,292 - Shew_0052 0.56 -0.1 64,292 - Shew_0052 0.56 -0.0 64,326 + Shew_0052 0.59 +0.9 64,334 + Shew_0052 0.60 +0.0 64,611 + -0.2 64,619 - +0.2 64,673 - -0.9 64,701 + -0.1 64,836 + Shew_0053 0.14 +1.1 64,844 - Shew_0053 0.15 -0.6 64,919 - Shew_0053 0.24 +0.3 64,972 - Shew_0053 0.30 +0.6 64,990 + Shew_0053 0.32 +0.2 64,998 - Shew_0053 0.33 +0.0 65,037 - Shew_0053 0.38 +0.7 65,053 - Shew_0053 0.40 +0.5 65,053 - Shew_0053 0.40 +1.2 65,055 + Shew_0053 0.40 +0.7 65,055 + Shew_0053 0.40 +0.9 65,055 + Shew_0053 0.40 +0.7 65,055 + Shew_0053 0.40 +0.3 65,055 + Shew_0053 0.40 +0.8 65,062 + Shew_0053 0.41 +0.9 65,063 - Shew_0053 0.41 +0.5 65,063 - Shew_0053 0.41 +0.9 65,063 - Shew_0053 0.41 +1.2 65,070 - Shew_0053 0.42 +0.9 65,199 - Shew_0053 0.57 +1.0 65,394 + Shew_0053 0.80 +1.2 65,528 + +0.9 65,536 - +0.6 65,594 + -0.1 65,720 - +0.4 65,991 + Shew_0054 0.28 +0.3 66,021 - Shew_0054 0.35 +0.4 66,063 - Shew_0054 0.44 +0.7 66,178 - Shew_0054 0.68 +0.4 66,258 + Shew_0054 0.85 +1.3 66,291 - +0.1 66,362 + +0.7 66,486 + Shew_0055 0.27 +0.9 66,545 + Shew_0055 0.37 +0.4 66,553 - Shew_0055 0.38 +0.8
Or see this region's nucleotide sequence