Experiment: Polymyxin B sulfate 20 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_0044 and Shew_0045 overlap by 4 nucleotides Shew_0045 and Shew_0046 are separated by 181 nucleotides
Shew_0044: Shew_0044 - acriflavin resistance protein (RefSeq), at 51,810 to 54,920
_0044
Shew_0045: Shew_0045 - RND family efflux transporter MFP subunit (RefSeq), at 54,917 to 55,987
_0045
Shew_0046: Shew_0046 - molybdenum cofactor synthesis domain-containing protein (RefSeq), at 56,169 to 57,416
_0046
Position (kb)
54
55
56 Strain fitness (log2 ratio)
-1
0
1
2
3 at 54.009 kb on - strand, within Shew_0044 at 54.009 kb on - strand, within Shew_0044 at 54.105 kb on + strand, within Shew_0044 at 54.113 kb on - strand, within Shew_0044 at 54.689 kb on - strand at 54.689 kb on - strand at 54.689 kb on - strand at 55.077 kb on + strand, within Shew_0045 at 55.085 kb on - strand, within Shew_0045 at 55.356 kb on - strand, within Shew_0045 at 55.560 kb on + strand, within Shew_0045 at 55.648 kb on - strand, within Shew_0045 at 55.746 kb on + strand, within Shew_0045 at 55.749 kb on + strand, within Shew_0045 at 55.749 kb on + strand, within Shew_0045 at 55.749 kb on + strand, within Shew_0045 at 55.757 kb on - strand, within Shew_0045 at 55.798 kb on + strand, within Shew_0045 at 55.891 kb on - strand at 56.100 kb on - strand at 56.100 kb on - strand at 56.418 kb on + strand, within Shew_0046 at 56.517 kb on + strand, within Shew_0046 at 56.698 kb on + strand, within Shew_0046 at 56.717 kb on + strand, within Shew_0046 at 56.725 kb on - strand, within Shew_0046
Per-strain Table
Position Strand Gene LocusTag Fraction Polymyxin B sulfate 20 mg/ml remove 54,009 - Shew_0044 0.71 +1.4 54,009 - Shew_0044 0.71 -0.6 54,105 + Shew_0044 0.74 -1.3 54,113 - Shew_0044 0.74 -0.2 54,689 - -1.0 54,689 - +0.6 54,689 - +0.1 55,077 + Shew_0045 0.15 +3.0 55,085 - Shew_0045 0.16 -0.0 55,356 - Shew_0045 0.41 +0.2 55,560 + Shew_0045 0.60 -0.5 55,648 - Shew_0045 0.68 +0.1 55,746 + Shew_0045 0.77 -0.3 55,749 + Shew_0045 0.78 +0.1 55,749 + Shew_0045 0.78 +2.3 55,749 + Shew_0045 0.78 +0.5 55,757 - Shew_0045 0.78 +1.1 55,798 + Shew_0045 0.82 +0.4 55,891 - +0.7 56,100 - +0.6 56,100 - +0.9 56,418 + Shew_0046 0.20 +0.7 56,517 + Shew_0046 0.28 +0.9 56,698 + Shew_0046 0.42 +0.7 56,717 + Shew_0046 0.44 +0.6 56,725 - Shew_0046 0.45 +0.6
Or see this region's nucleotide sequence