Experiment: Polymyxin B sulfate 20 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_0031 and Shew_0032 are separated by 9 nucleotides Shew_0032 and Shew_0033 are separated by 222 nucleotides
Shew_0031: Shew_0031 - RND family efflux transporter MFP subunit (RefSeq), at 33,256 to 34,416
_0031
Shew_0032: Shew_0032 - hydrophobe/amphiphile efflux-1 (HAE1) family protein (RefSeq), at 34,426 to 37,575
_0032
Shew_0033: Shew_0033 - peptidase M14, carboxypeptidase A (RefSeq), at 37,798 to 39,639
_0033
Position (kb) 34
35
36
37
38 Strain fitness (log2 ratio) -6
-5
-4
-3
-2
-1
0
1 at 33.426 kb on + strand, within Shew_0031 at 33.432 kb on + strand, within Shew_0031 at 33.526 kb on - strand, within Shew_0031 at 33.559 kb on + strand, within Shew_0031 at 33.588 kb on - strand, within Shew_0031 at 33.618 kb on + strand, within Shew_0031 at 33.626 kb on - strand, within Shew_0031 at 33.626 kb on - strand, within Shew_0031 at 33.849 kb on - strand, within Shew_0031 at 33.943 kb on - strand, within Shew_0031 at 34.358 kb on + strand at 34.366 kb on - strand at 34.565 kb on - strand at 34.585 kb on + strand at 34.802 kb on + strand, within Shew_0032 at 34.802 kb on + strand, within Shew_0032 at 34.810 kb on - strand, within Shew_0032 at 34.840 kb on + strand, within Shew_0032 at 34.985 kb on + strand, within Shew_0032 at 34.985 kb on + strand, within Shew_0032 at 34.985 kb on + strand, within Shew_0032 at 34.993 kb on - strand, within Shew_0032 at 35.223 kb on - strand, within Shew_0032 at 35.313 kb on + strand, within Shew_0032 at 35.384 kb on + strand, within Shew_0032 at 35.395 kb on + strand, within Shew_0032 at 35.561 kb on - strand, within Shew_0032 at 35.762 kb on + strand, within Shew_0032 at 35.877 kb on - strand, within Shew_0032 at 36.056 kb on + strand, within Shew_0032 at 36.262 kb on + strand, within Shew_0032 at 36.548 kb on + strand, within Shew_0032 at 37.182 kb on + strand, within Shew_0032 at 37.182 kb on + strand, within Shew_0032 at 37.207 kb on + strand, within Shew_0032 at 37.296 kb on + strand at 37.960 kb on + strand at 37.968 kb on - strand at 38.118 kb on - strand, within Shew_0033 at 38.295 kb on + strand, within Shew_0033 at 38.564 kb on + strand, within Shew_0033
Per-strain Table
Position Strand Gene LocusTag Fraction Polymyxin B sulfate 20 mg/ml remove 33,426 + Shew_0031 0.15 -4.0 33,432 + Shew_0031 0.15 -3.0 33,526 - Shew_0031 0.23 -4.1 33,559 + Shew_0031 0.26 -3.0 33,588 - Shew_0031 0.29 -2.5 33,618 + Shew_0031 0.31 -3.6 33,626 - Shew_0031 0.32 -1.2 33,626 - Shew_0031 0.32 -4.9 33,849 - Shew_0031 0.51 -4.3 33,943 - Shew_0031 0.59 -4.5 34,358 + -4.2 34,366 - -4.9 34,565 - -6.0 34,585 + -3.4 34,802 + Shew_0032 0.12 -3.8 34,802 + Shew_0032 0.12 -1.7 34,810 - Shew_0032 0.12 -3.2 34,840 + Shew_0032 0.13 -1.0 34,985 + Shew_0032 0.18 -2.5 34,985 + Shew_0032 0.18 -2.9 34,985 + Shew_0032 0.18 -4.1 34,993 - Shew_0032 0.18 -3.8 35,223 - Shew_0032 0.25 -2.7 35,313 + Shew_0032 0.28 -2.4 35,384 + Shew_0032 0.30 -2.5 35,395 + Shew_0032 0.31 -3.9 35,561 - Shew_0032 0.36 -3.7 35,762 + Shew_0032 0.42 -3.9 35,877 - Shew_0032 0.46 -4.7 36,056 + Shew_0032 0.52 -2.4 36,262 + Shew_0032 0.58 -4.1 36,548 + Shew_0032 0.67 -4.5 37,182 + Shew_0032 0.87 -2.9 37,182 + Shew_0032 0.87 -2.5 37,207 + Shew_0032 0.88 -4.1 37,296 + -3.8 37,960 + -0.1 37,968 - -0.6 38,118 - Shew_0033 0.17 -0.2 38,295 + Shew_0033 0.27 +0.0 38,564 + Shew_0033 0.42 -1.8
Or see this region's nucleotide sequence