Experiment: Polymyxin B sulfate 20 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_0030 and Shew_0031 are separated by 424 nucleotides Shew_0031 and Shew_0032 are separated by 9 nucleotides
Shew_0030: Shew_0030 - hypothetical protein (RefSeq), at 32,076 to 32,831
_0030
Shew_0031: Shew_0031 - RND family efflux transporter MFP subunit (RefSeq), at 33,256 to 34,416
_0031
Shew_0032: Shew_0032 - hydrophobe/amphiphile efflux-1 (HAE1) family protein (RefSeq), at 34,426 to 37,575
_0032
Position (kb)
33
34
35 Strain fitness (log2 ratio)
-6
-5
-4
-3
-2
-1
0
1 at 32.285 kb on + strand, within Shew_0030 at 32.293 kb on - strand, within Shew_0030 at 32.293 kb on - strand, within Shew_0030 at 32.336 kb on - strand, within Shew_0030 at 32.585 kb on + strand, within Shew_0030 at 32.604 kb on + strand, within Shew_0030 at 32.815 kb on - strand at 32.815 kb on - strand at 32.862 kb on - strand at 33.122 kb on + strand at 33.148 kb on + strand at 33.374 kb on - strand, within Shew_0031 at 33.426 kb on + strand, within Shew_0031 at 33.432 kb on + strand, within Shew_0031 at 33.526 kb on - strand, within Shew_0031 at 33.559 kb on + strand, within Shew_0031 at 33.588 kb on - strand, within Shew_0031 at 33.618 kb on + strand, within Shew_0031 at 33.626 kb on - strand, within Shew_0031 at 33.626 kb on - strand, within Shew_0031 at 33.849 kb on - strand, within Shew_0031 at 33.943 kb on - strand, within Shew_0031 at 34.358 kb on + strand at 34.366 kb on - strand at 34.565 kb on - strand at 34.585 kb on + strand at 34.802 kb on + strand, within Shew_0032 at 34.802 kb on + strand, within Shew_0032 at 34.810 kb on - strand, within Shew_0032 at 34.840 kb on + strand, within Shew_0032 at 34.985 kb on + strand, within Shew_0032 at 34.985 kb on + strand, within Shew_0032 at 34.985 kb on + strand, within Shew_0032 at 34.993 kb on - strand, within Shew_0032 at 35.223 kb on - strand, within Shew_0032 at 35.313 kb on + strand, within Shew_0032 at 35.384 kb on + strand, within Shew_0032 at 35.395 kb on + strand, within Shew_0032
Per-strain Table
Position Strand Gene LocusTag Fraction Polymyxin B sulfate 20 mg/ml remove 32,285 + Shew_0030 0.28 -0.3 32,293 - Shew_0030 0.29 -0.1 32,293 - Shew_0030 0.29 -0.2 32,336 - Shew_0030 0.34 +0.5 32,585 + Shew_0030 0.67 +0.1 32,604 + Shew_0030 0.70 +0.2 32,815 - -0.1 32,815 - -0.0 32,862 - +0.7 33,122 + -4.4 33,148 + -2.7 33,374 - Shew_0031 0.10 -2.3 33,426 + Shew_0031 0.15 -4.0 33,432 + Shew_0031 0.15 -3.0 33,526 - Shew_0031 0.23 -4.1 33,559 + Shew_0031 0.26 -3.0 33,588 - Shew_0031 0.29 -2.5 33,618 + Shew_0031 0.31 -3.6 33,626 - Shew_0031 0.32 -1.2 33,626 - Shew_0031 0.32 -4.9 33,849 - Shew_0031 0.51 -4.3 33,943 - Shew_0031 0.59 -4.5 34,358 + -4.2 34,366 - -4.9 34,565 - -6.0 34,585 + -3.4 34,802 + Shew_0032 0.12 -3.8 34,802 + Shew_0032 0.12 -1.7 34,810 - Shew_0032 0.12 -3.2 34,840 + Shew_0032 0.13 -1.0 34,985 + Shew_0032 0.18 -2.5 34,985 + Shew_0032 0.18 -2.9 34,985 + Shew_0032 0.18 -4.1 34,993 - Shew_0032 0.18 -3.8 35,223 - Shew_0032 0.25 -2.7 35,313 + Shew_0032 0.28 -2.4 35,384 + Shew_0032 0.30 -2.5 35,395 + Shew_0032 0.31 -3.9
Or see this region's nucleotide sequence