Experiment: H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 FREE
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS05150 and MMJJ_RS05155 are separated by 115 nucleotides MMJJ_RS05155 and MMJJ_RS05160 are separated by 107 nucleotides MMJJ_RS05160 and MMJJ_RS05165 are separated by 13 nucleotides
MMJJ_RS05150: MMJJ_RS05150 - 5-amino-6-(D-ribitylamino)uracil--L-tyrosine 4-hydroxyphenyl transferase CofH, at 953,284 to 954,360
_RS05150
MMJJ_RS05155: MMJJ_RS05155 - 5-amino-6-(D-ribitylamino)uracil--L-tyrosine 4-hydroxyphenyl transferase CofH, at 954,476 to 955,555
_RS05155
MMJJ_RS05160: MMJJ_RS05160 - RNA-binding protein, at 955,663 to 956,142
_RS05160
MMJJ_RS05165: MMJJ_RS05165 - class I SAM-dependent methyltransferase, at 956,156 to 956,764
_RS05165
Position (kb) 954
955
956 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 953.553 kb on - strand, within MMJJ_RS05150 at 953.575 kb on + strand, within MMJJ_RS05150 at 953.584 kb on + strand, within MMJJ_RS05150 at 953.643 kb on - strand, within MMJJ_RS05150 at 953.643 kb on - strand, within MMJJ_RS05150 at 953.644 kb on + strand, within MMJJ_RS05150 at 953.679 kb on + strand, within MMJJ_RS05150 at 953.680 kb on - strand, within MMJJ_RS05150 at 953.680 kb on - strand, within MMJJ_RS05150 at 953.680 kb on - strand, within MMJJ_RS05150 at 953.680 kb on - strand, within MMJJ_RS05150 at 953.680 kb on - strand, within MMJJ_RS05150 at 953.681 kb on + strand, within MMJJ_RS05150 at 953.681 kb on + strand, within MMJJ_RS05150 at 953.692 kb on - strand, within MMJJ_RS05150 at 953.736 kb on - strand, within MMJJ_RS05150 at 953.736 kb on - strand, within MMJJ_RS05150 at 953.737 kb on + strand, within MMJJ_RS05150 at 953.822 kb on - strand, within MMJJ_RS05150 at 953.823 kb on + strand, within MMJJ_RS05150 at 953.823 kb on + strand, within MMJJ_RS05150 at 953.871 kb on - strand, within MMJJ_RS05150 at 953.872 kb on + strand, within MMJJ_RS05150 at 953.872 kb on + strand, within MMJJ_RS05150 at 953.901 kb on - strand, within MMJJ_RS05150 at 953.902 kb on + strand, within MMJJ_RS05150 at 953.950 kb on + strand, within MMJJ_RS05150 at 954.029 kb on - strand, within MMJJ_RS05150 at 954.030 kb on + strand, within MMJJ_RS05150 at 954.066 kb on - strand, within MMJJ_RS05150 at 954.088 kb on - strand, within MMJJ_RS05150 at 954.127 kb on + strand, within MMJJ_RS05150 at 954.135 kb on - strand, within MMJJ_RS05150 at 954.238 kb on + strand, within MMJJ_RS05150 at 954.261 kb on - strand at 954.262 kb on + strand at 954.262 kb on + strand at 954.265 kb on - strand at 954.265 kb on - strand at 954.266 kb on + strand at 954.266 kb on + strand at 954.281 kb on - strand at 954.287 kb on - strand at 954.288 kb on + strand at 954.303 kb on - strand at 954.304 kb on + strand at 954.304 kb on + strand at 954.350 kb on - strand at 954.351 kb on + strand at 954.359 kb on + strand at 954.389 kb on - strand at 954.390 kb on + strand at 954.400 kb on - strand at 954.401 kb on + strand at 954.401 kb on + strand at 954.451 kb on - strand at 954.452 kb on + strand at 954.456 kb on + strand at 954.614 kb on - strand, within MMJJ_RS05155 at 954.614 kb on - strand, within MMJJ_RS05155 at 954.614 kb on - strand, within MMJJ_RS05155 at 954.615 kb on + strand, within MMJJ_RS05155 at 954.632 kb on + strand at 954.812 kb on - strand, within MMJJ_RS05155 at 954.927 kb on - strand, within MMJJ_RS05155 at 955.014 kb on - strand, within MMJJ_RS05155 at 955.015 kb on + strand, within MMJJ_RS05155 at 955.325 kb on - strand, within MMJJ_RS05155 at 955.325 kb on - strand, within MMJJ_RS05155 at 955.325 kb on - strand, within MMJJ_RS05155 at 955.325 kb on - strand, within MMJJ_RS05155 at 955.371 kb on - strand, within MMJJ_RS05155 at 955.467 kb on + strand at 955.467 kb on + strand at 955.500 kb on - strand at 955.549 kb on + strand at 955.553 kb on - strand at 955.553 kb on - strand at 955.553 kb on - strand at 955.553 kb on - strand at 955.554 kb on + strand at 955.562 kb on - strand at 955.563 kb on + strand at 955.563 kb on + strand at 955.591 kb on - strand at 955.592 kb on + strand at 955.597 kb on - strand at 955.598 kb on + strand at 955.598 kb on + strand at 955.598 kb on + strand at 955.601 kb on - strand at 955.602 kb on + strand at 955.651 kb on + strand at 955.681 kb on - strand at 955.681 kb on - strand at 955.682 kb on + strand at 955.682 kb on + strand at 955.850 kb on - strand at 955.851 kb on + strand, within MMJJ_RS05160 at 955.902 kb on - strand, within MMJJ_RS05160 at 955.907 kb on - strand, within MMJJ_RS05160 at 955.985 kb on - strand, within MMJJ_RS05160 at 956.161 kb on + strand at 956.190 kb on - strand at 956.191 kb on + strand at 956.203 kb on - strand at 956.208 kb on - strand at 956.239 kb on + strand, within MMJJ_RS05165 at 956.342 kb on + strand, within MMJJ_RS05165 at 956.355 kb on - strand, within MMJJ_RS05165 at 956.355 kb on - strand, within MMJJ_RS05165 at 956.428 kb on - strand, within MMJJ_RS05165 at 956.428 kb on - strand, within MMJJ_RS05165 at 956.429 kb on + strand, within MMJJ_RS05165 at 956.452 kb on - strand, within MMJJ_RS05165 at 956.473 kb on + strand, within MMJJ_RS05165 at 956.478 kb on - strand, within MMJJ_RS05165 at 956.492 kb on - strand, within MMJJ_RS05165 at 956.493 kb on + strand, within MMJJ_RS05165
Per-strain Table
Position Strand Gene LocusTag Fraction H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 FREE remove 953,553 - MMJJ_RS05150 0.25 +0.1 953,575 + MMJJ_RS05150 0.27 -0.7 953,584 + MMJJ_RS05150 0.28 +0.2 953,643 - MMJJ_RS05150 0.33 -0.1 953,643 - MMJJ_RS05150 0.33 -1.1 953,644 + MMJJ_RS05150 0.33 +0.5 953,679 + MMJJ_RS05150 0.37 -0.3 953,680 - MMJJ_RS05150 0.37 +0.4 953,680 - MMJJ_RS05150 0.37 -0.5 953,680 - MMJJ_RS05150 0.37 -0.8 953,680 - MMJJ_RS05150 0.37 +0.1 953,680 - MMJJ_RS05150 0.37 +0.5 953,681 + MMJJ_RS05150 0.37 +0.6 953,681 + MMJJ_RS05150 0.37 +1.3 953,692 - MMJJ_RS05150 0.38 -0.2 953,736 - MMJJ_RS05150 0.42 -0.1 953,736 - MMJJ_RS05150 0.42 -0.2 953,737 + MMJJ_RS05150 0.42 +0.8 953,822 - MMJJ_RS05150 0.50 -0.2 953,823 + MMJJ_RS05150 0.50 +0.7 953,823 + MMJJ_RS05150 0.50 +0.8 953,871 - MMJJ_RS05150 0.55 +1.3 953,872 + MMJJ_RS05150 0.55 +0.5 953,872 + MMJJ_RS05150 0.55 +1.7 953,901 - MMJJ_RS05150 0.57 +0.8 953,902 + MMJJ_RS05150 0.57 +0.4 953,950 + MMJJ_RS05150 0.62 +0.1 954,029 - MMJJ_RS05150 0.69 +0.2 954,030 + MMJJ_RS05150 0.69 +0.5 954,066 - MMJJ_RS05150 0.73 +0.3 954,088 - MMJJ_RS05150 0.75 +0.8 954,127 + MMJJ_RS05150 0.78 -0.3 954,135 - MMJJ_RS05150 0.79 -0.8 954,238 + MMJJ_RS05150 0.89 +0.8 954,261 - -0.3 954,262 + +0.2 954,262 + -0.8 954,265 - -0.7 954,265 - -1.5 954,266 + +0.8 954,266 + +0.3 954,281 - -0.6 954,287 - -0.2 954,288 + +0.0 954,303 - +1.9 954,304 + +0.3 954,304 + -1.2 954,350 - -0.2 954,351 + -0.3 954,359 + -0.1 954,389 - -0.5 954,390 + +1.0 954,400 - -0.9 954,401 + +0.2 954,401 + -0.3 954,451 - -2.1 954,452 + +1.6 954,456 + -2.8 954,614 - MMJJ_RS05155 0.13 -2.9 954,614 - MMJJ_RS05155 0.13 -1.9 954,614 - MMJJ_RS05155 0.13 -1.7 954,615 + MMJJ_RS05155 0.13 -1.8 954,632 + -1.8 954,812 - MMJJ_RS05155 0.31 -2.7 954,927 - MMJJ_RS05155 0.42 -2.5 955,014 - MMJJ_RS05155 0.50 -3.1 955,015 + MMJJ_RS05155 0.50 -2.8 955,325 - MMJJ_RS05155 0.79 -0.8 955,325 - MMJJ_RS05155 0.79 -3.2 955,325 - MMJJ_RS05155 0.79 -2.1 955,325 - MMJJ_RS05155 0.79 -3.4 955,371 - MMJJ_RS05155 0.83 -3.3 955,467 + -2.5 955,467 + -0.3 955,500 - -2.2 955,549 + +0.9 955,553 - +1.2 955,553 - +1.5 955,553 - +1.4 955,553 - -1.1 955,554 + +1.1 955,562 - +0.9 955,563 + +0.2 955,563 + -0.0 955,591 - -2.1 955,592 + +0.0 955,597 - -2.5 955,598 + -2.2 955,598 + -0.3 955,598 + -1.0 955,601 - -2.6 955,602 + -0.7 955,651 + -1.2 955,681 - -1.0 955,681 - -1.7 955,682 + -2.6 955,682 + -4.3 955,850 - +1.3 955,851 + MMJJ_RS05160 0.39 -3.3 955,902 - MMJJ_RS05160 0.50 -1.5 955,907 - MMJJ_RS05160 0.51 -1.1 955,985 - MMJJ_RS05160 0.67 -1.2 956,161 + -0.3 956,190 - -3.5 956,191 + -1.5 956,203 - -3.5 956,208 - -2.1 956,239 + MMJJ_RS05165 0.14 -1.2 956,342 + MMJJ_RS05165 0.31 -2.4 956,355 - MMJJ_RS05165 0.33 -3.4 956,355 - MMJJ_RS05165 0.33 -3.0 956,428 - MMJJ_RS05165 0.45 -3.2 956,428 - MMJJ_RS05165 0.45 -2.8 956,429 + MMJJ_RS05165 0.45 -1.3 956,452 - MMJJ_RS05165 0.49 -0.5 956,473 + MMJJ_RS05165 0.52 -1.6 956,478 - MMJJ_RS05165 0.53 -1.0 956,492 - MMJJ_RS05165 0.55 -2.0 956,493 + MMJJ_RS05165 0.55 -2.3
Or see this region's nucleotide sequence