Experiment: H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 FREE
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS01995 and MMJJ_RS02000 are separated by 76 nucleotides MMJJ_RS02000 and MMJJ_RS02005 overlap by 8 nucleotides MMJJ_RS02005 and MMJJ_RS02010 are separated by 145 nucleotides
MMJJ_RS01995: MMJJ_RS01995 - valine--tRNA ligase, at 381,164 to 383,824
_RS01995
MMJJ_RS02000: MMJJ_RS02000 - AzlD domain-containing protein, at 383,901 to 384,209
_RS02000
MMJJ_RS02005: MMJJ_RS02005 - AzlC family ABC transporter permease, at 384,202 to 384,918
_RS02005
MMJJ_RS02010: MMJJ_RS02010 - MBL fold metallo-hydrolase, at 385,064 to 385,846
_RS02010
Position (kb) 384
385 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 383.740 kb on + strand at 383.834 kb on + strand at 383.903 kb on + strand at 383.923 kb on - strand at 383.975 kb on + strand, within MMJJ_RS02000 at 383.975 kb on + strand, within MMJJ_RS02000 at 383.975 kb on + strand, within MMJJ_RS02000 at 383.975 kb on + strand, within MMJJ_RS02000 at 384.035 kb on + strand, within MMJJ_RS02000 at 384.035 kb on + strand, within MMJJ_RS02000 at 384.082 kb on - strand, within MMJJ_RS02000 at 384.156 kb on + strand, within MMJJ_RS02000 at 384.157 kb on - strand, within MMJJ_RS02000 at 384.157 kb on - strand, within MMJJ_RS02000 at 384.157 kb on - strand, within MMJJ_RS02000 at 384.157 kb on - strand, within MMJJ_RS02000 at 384.158 kb on + strand, within MMJJ_RS02000 at 384.178 kb on + strand, within MMJJ_RS02000 at 384.181 kb on + strand at 384.183 kb on - strand at 384.183 kb on - strand at 384.183 kb on - strand at 384.183 kb on - strand at 384.186 kb on - strand at 384.223 kb on - strand at 384.226 kb on + strand at 384.267 kb on - strand at 384.268 kb on + strand at 384.271 kb on - strand at 384.328 kb on + strand, within MMJJ_RS02005 at 384.331 kb on - strand, within MMJJ_RS02005 at 384.386 kb on + strand, within MMJJ_RS02005 at 384.403 kb on - strand, within MMJJ_RS02005 at 384.403 kb on - strand, within MMJJ_RS02005 at 384.404 kb on + strand, within MMJJ_RS02005 at 384.407 kb on - strand, within MMJJ_RS02005 at 384.408 kb on + strand, within MMJJ_RS02005 at 384.408 kb on + strand, within MMJJ_RS02005 at 384.408 kb on + strand at 384.424 kb on + strand, within MMJJ_RS02005 at 384.427 kb on + strand, within MMJJ_RS02005 at 384.427 kb on + strand, within MMJJ_RS02005 at 384.427 kb on + strand, within MMJJ_RS02005 at 384.427 kb on + strand, within MMJJ_RS02005 at 384.433 kb on - strand, within MMJJ_RS02005 at 384.434 kb on + strand, within MMJJ_RS02005 at 384.681 kb on - strand, within MMJJ_RS02005 at 384.727 kb on - strand, within MMJJ_RS02005 at 384.764 kb on + strand, within MMJJ_RS02005 at 384.764 kb on + strand, within MMJJ_RS02005 at 384.860 kb on + strand at 384.907 kb on - strand at 384.919 kb on + strand at 384.947 kb on - strand at 384.953 kb on + strand at 384.978 kb on + strand at 384.978 kb on + strand at 384.978 kb on + strand at 384.995 kb on - strand at 384.996 kb on + strand at 384.996 kb on + strand at 384.996 kb on + strand at 384.997 kb on - strand at 384.997 kb on - strand at 384.997 kb on - strand at 384.997 kb on - strand at 384.997 kb on - strand at 384.997 kb on - strand at 384.997 kb on - strand at 384.997 kb on - strand at 384.997 kb on - strand at 384.998 kb on + strand at 384.998 kb on + strand at 384.998 kb on + strand at 384.998 kb on + strand at 384.999 kb on - strand at 385.000 kb on + strand at 385.000 kb on + strand at 385.000 kb on + strand at 385.000 kb on + strand at 385.009 kb on + strand at 385.010 kb on - strand at 385.011 kb on + strand at 385.011 kb on + strand at 385.011 kb on + strand at 385.013 kb on + strand at 385.013 kb on + strand at 385.030 kb on + strand at 385.031 kb on - strand at 385.031 kb on - strand at 385.032 kb on + strand at 385.032 kb on + strand at 385.032 kb on + strand at 385.033 kb on - strand at 385.034 kb on + strand at 385.036 kb on - strand at 385.041 kb on - strand at 385.109 kb on - strand at 385.118 kb on - strand at 385.118 kb on - strand at 385.118 kb on - strand at 385.118 kb on - strand at 385.209 kb on - strand, within MMJJ_RS02010 at 385.282 kb on - strand, within MMJJ_RS02010 at 385.283 kb on + strand, within MMJJ_RS02010 at 385.285 kb on - strand, within MMJJ_RS02010 at 385.286 kb on + strand, within MMJJ_RS02010 at 385.292 kb on + strand, within MMJJ_RS02010 at 385.296 kb on + strand, within MMJJ_RS02010 at 385.325 kb on - strand, within MMJJ_RS02010 at 385.358 kb on - strand, within MMJJ_RS02010 at 385.363 kb on - strand, within MMJJ_RS02010 at 385.518 kb on + strand, within MMJJ_RS02010 at 385.518 kb on + strand, within MMJJ_RS02010 at 385.519 kb on - strand, within MMJJ_RS02010 at 385.520 kb on + strand, within MMJJ_RS02010 at 385.520 kb on + strand, within MMJJ_RS02010 at 385.540 kb on + strand, within MMJJ_RS02010 at 385.540 kb on + strand, within MMJJ_RS02010 at 385.559 kb on + strand, within MMJJ_RS02010 at 385.582 kb on - strand, within MMJJ_RS02010 at 385.583 kb on + strand, within MMJJ_RS02010 at 385.612 kb on + strand, within MMJJ_RS02010 at 385.627 kb on + strand, within MMJJ_RS02010 at 385.631 kb on + strand, within MMJJ_RS02010 at 385.631 kb on + strand, within MMJJ_RS02010 at 385.671 kb on + strand, within MMJJ_RS02010 at 385.706 kb on - strand, within MMJJ_RS02010 at 385.751 kb on - strand, within MMJJ_RS02010 at 385.752 kb on + strand, within MMJJ_RS02010 at 385.772 kb on - strand at 385.775 kb on + strand at 385.800 kb on + strand at 385.820 kb on + strand at 385.826 kb on - strand at 385.827 kb on + strand at 385.827 kb on + strand at 385.827 kb on + strand at 385.832 kb on - strand at 385.832 kb on - strand at 385.832 kb on - strand at 385.838 kb on - strand at 385.839 kb on + strand at 385.866 kb on - strand at 385.870 kb on - strand at 385.870 kb on - strand at 385.870 kb on - strand at 385.871 kb on + strand at 385.871 kb on + strand at 385.885 kb on - strand at 385.886 kb on + strand at 385.887 kb on - strand at 385.887 kb on - strand at 385.888 kb on + strand at 385.889 kb on - strand at 385.889 kb on - strand at 385.890 kb on + strand at 385.890 kb on + strand at 385.892 kb on - strand at 385.892 kb on - strand at 385.892 kb on - strand at 385.893 kb on + strand at 385.894 kb on - strand at 385.894 kb on - strand at 385.894 kb on - strand at 385.895 kb on + strand at 385.895 kb on + strand at 385.895 kb on + strand at 385.900 kb on - strand at 385.909 kb on - strand at 385.910 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 FREE remove 383,740 + +0.1 383,834 + -0.8 383,903 + +1.6 383,923 - +1.7 383,975 + MMJJ_RS02000 0.24 +0.6 383,975 + MMJJ_RS02000 0.24 +0.1 383,975 + MMJJ_RS02000 0.24 +0.3 383,975 + MMJJ_RS02000 0.24 +2.3 384,035 + MMJJ_RS02000 0.43 +0.5 384,035 + MMJJ_RS02000 0.43 -0.8 384,082 - MMJJ_RS02000 0.59 +0.1 384,156 + MMJJ_RS02000 0.83 -1.2 384,157 - MMJJ_RS02000 0.83 +1.9 384,157 - MMJJ_RS02000 0.83 +0.3 384,157 - MMJJ_RS02000 0.83 +0.4 384,157 - MMJJ_RS02000 0.83 +0.7 384,158 + MMJJ_RS02000 0.83 -1.6 384,178 + MMJJ_RS02000 0.90 -0.1 384,181 + +0.4 384,183 - +0.1 384,183 - -0.4 384,183 - +1.2 384,183 - +2.1 384,186 - +0.1 384,223 - +0.3 384,226 + +2.1 384,267 - -0.5 384,268 + -0.6 384,271 - +0.3 384,328 + MMJJ_RS02005 0.18 -1.7 384,331 - MMJJ_RS02005 0.18 +0.4 384,386 + MMJJ_RS02005 0.26 -0.8 384,403 - MMJJ_RS02005 0.28 -0.1 384,403 - MMJJ_RS02005 0.28 +0.3 384,404 + MMJJ_RS02005 0.28 +0.6 384,407 - MMJJ_RS02005 0.29 +0.0 384,408 + MMJJ_RS02005 0.29 +0.9 384,408 + MMJJ_RS02005 0.29 +1.5 384,408 + -0.5 384,424 + MMJJ_RS02005 0.31 -0.1 384,427 + MMJJ_RS02005 0.31 -0.4 384,427 + MMJJ_RS02005 0.31 +1.6 384,427 + MMJJ_RS02005 0.31 -0.1 384,427 + MMJJ_RS02005 0.31 +0.8 384,433 - MMJJ_RS02005 0.32 +0.3 384,434 + MMJJ_RS02005 0.32 +1.0 384,681 - MMJJ_RS02005 0.67 +0.3 384,727 - MMJJ_RS02005 0.73 +0.6 384,764 + MMJJ_RS02005 0.78 +0.1 384,764 + MMJJ_RS02005 0.78 +0.2 384,860 + +0.1 384,907 - -0.4 384,919 + +0.1 384,947 - +0.8 384,953 + +0.1 384,978 + +0.7 384,978 + -0.1 384,978 + +0.5 384,995 - +0.1 384,996 + +0.7 384,996 + +0.4 384,996 + +0.4 384,997 - +0.3 384,997 - -1.3 384,997 - +0.4 384,997 - -0.9 384,997 - +1.9 384,997 - +0.3 384,997 - -0.1 384,997 - -1.1 384,997 - +0.7 384,998 + +0.3 384,998 + -1.0 384,998 + -0.7 384,998 + -0.4 384,999 - -0.0 385,000 + -0.3 385,000 + -1.2 385,000 + +0.8 385,000 + +0.3 385,009 + +1.0 385,010 - -0.6 385,011 + -0.5 385,011 + +1.5 385,011 + +1.0 385,013 + +1.7 385,013 + +0.3 385,030 + -1.0 385,031 - -0.0 385,031 - -1.7 385,032 + -0.0 385,032 + +0.4 385,032 + +0.6 385,033 - -0.1 385,034 + -1.3 385,036 - -0.1 385,041 - -1.0 385,109 - -0.2 385,118 - -0.7 385,118 - -0.2 385,118 - -0.5 385,118 - +0.3 385,209 - MMJJ_RS02010 0.19 -0.3 385,282 - MMJJ_RS02010 0.28 -0.9 385,283 + MMJJ_RS02010 0.28 +0.8 385,285 - MMJJ_RS02010 0.28 -0.5 385,286 + MMJJ_RS02010 0.28 +0.2 385,292 + MMJJ_RS02010 0.29 -0.5 385,296 + MMJJ_RS02010 0.30 +0.7 385,325 - MMJJ_RS02010 0.33 -0.5 385,358 - MMJJ_RS02010 0.38 +0.0 385,363 - MMJJ_RS02010 0.38 -0.3 385,518 + MMJJ_RS02010 0.58 +0.7 385,518 + MMJJ_RS02010 0.58 -0.4 385,519 - MMJJ_RS02010 0.58 +0.2 385,520 + MMJJ_RS02010 0.58 +0.6 385,520 + MMJJ_RS02010 0.58 +0.1 385,540 + MMJJ_RS02010 0.61 -1.2 385,540 + MMJJ_RS02010 0.61 -0.6 385,559 + MMJJ_RS02010 0.63 -0.0 385,582 - MMJJ_RS02010 0.66 +0.2 385,583 + MMJJ_RS02010 0.66 -0.1 385,612 + MMJJ_RS02010 0.70 -1.0 385,627 + MMJJ_RS02010 0.72 +1.2 385,631 + MMJJ_RS02010 0.72 +1.5 385,631 + MMJJ_RS02010 0.72 +1.1 385,671 + MMJJ_RS02010 0.78 -0.3 385,706 - MMJJ_RS02010 0.82 +0.6 385,751 - MMJJ_RS02010 0.88 +0.4 385,752 + MMJJ_RS02010 0.88 +1.4 385,772 - +0.4 385,775 + +0.1 385,800 + +0.1 385,820 + +1.0 385,826 - -0.4 385,827 + -0.4 385,827 + +1.7 385,827 + +0.5 385,832 - +1.5 385,832 - -0.2 385,832 - -1.1 385,838 - -0.2 385,839 + -0.5 385,866 - +2.9 385,870 - -0.9 385,870 - -1.7 385,870 - -1.1 385,871 + -0.6 385,871 + -0.0 385,885 - -0.1 385,886 + -0.6 385,887 - +0.6 385,887 - +0.3 385,888 + +0.4 385,889 - -1.6 385,889 - +0.1 385,890 + +0.9 385,890 + +0.5 385,892 - +0.2 385,892 - +0.1 385,892 - +0.5 385,893 + -0.3 385,894 - -1.8 385,894 - -2.7 385,894 - +1.9 385,895 + +0.7 385,895 + +0.6 385,895 + -0.9 385,900 - -2.1 385,909 - +0.2 385,910 + -0.5
Or see this region's nucleotide sequence