Experiment: H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 FREE
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS01370 and MMJJ_RS01375 are separated by 13 nucleotides MMJJ_RS01375 and MMJJ_RS01380 are separated by 39 nucleotides MMJJ_RS01380 and MMJJ_RS01385 are separated by 194 nucleotides MMJJ_RS01385 and MMJJ_RS01390 are separated by 23 nucleotides
MMJJ_RS01370: MMJJ_RS01370 - MnmC family methyltransferase, at 264,942 to 265,964
_RS01370
MMJJ_RS01375: MMJJ_RS01375 - tetrahydromethanopterin:alpha-L-glutamate ligase, at 265,978 to 266,856
_RS01375
MMJJ_RS01380: MMJJ_RS01380 - DUF1890 domain-containing protein, at 266,896 to 267,339
_RS01380
MMJJ_RS01385: MMJJ_RS01385 - PadR family transcriptional regulator, at 267,534 to 268,085
_RS01385
MMJJ_RS01390: MMJJ_RS01390 - MATE family efflux transporter, at 268,109 to 269,452
_RS01390
Position (kb) 266
267
268 Strain fitness (log2 ratio) -1
0
1
2
3 at 265.913 kb on + strand at 265.951 kb on + strand at 265.979 kb on - strand at 266.032 kb on - strand at 266.033 kb on + strand at 266.078 kb on + strand, within MMJJ_RS01375 at 266.114 kb on + strand, within MMJJ_RS01375 at 266.114 kb on + strand, within MMJJ_RS01375 at 266.119 kb on - strand, within MMJJ_RS01375 at 266.119 kb on - strand, within MMJJ_RS01375 at 266.119 kb on - strand, within MMJJ_RS01375 at 266.120 kb on + strand, within MMJJ_RS01375 at 266.174 kb on + strand, within MMJJ_RS01375 at 266.247 kb on - strand, within MMJJ_RS01375 at 266.250 kb on + strand, within MMJJ_RS01375 at 266.336 kb on - strand, within MMJJ_RS01375 at 266.337 kb on + strand, within MMJJ_RS01375 at 266.337 kb on + strand, within MMJJ_RS01375 at 266.358 kb on - strand, within MMJJ_RS01375 at 266.398 kb on + strand, within MMJJ_RS01375 at 266.410 kb on - strand, within MMJJ_RS01375 at 266.411 kb on + strand, within MMJJ_RS01375 at 266.411 kb on + strand, within MMJJ_RS01375 at 266.447 kb on + strand, within MMJJ_RS01375 at 266.466 kb on - strand, within MMJJ_RS01375 at 266.562 kb on - strand, within MMJJ_RS01375 at 266.599 kb on - strand, within MMJJ_RS01375 at 266.599 kb on - strand, within MMJJ_RS01375 at 266.600 kb on + strand, within MMJJ_RS01375 at 266.605 kb on - strand, within MMJJ_RS01375 at 266.605 kb on - strand, within MMJJ_RS01375 at 266.605 kb on - strand, within MMJJ_RS01375 at 266.605 kb on - strand, within MMJJ_RS01375 at 266.609 kb on - strand, within MMJJ_RS01375 at 266.621 kb on - strand, within MMJJ_RS01375 at 266.621 kb on - strand, within MMJJ_RS01375 at 266.621 kb on - strand, within MMJJ_RS01375 at 266.626 kb on - strand, within MMJJ_RS01375 at 266.627 kb on + strand, within MMJJ_RS01375 at 266.627 kb on + strand, within MMJJ_RS01375 at 266.674 kb on + strand, within MMJJ_RS01375 at 266.706 kb on - strand, within MMJJ_RS01375 at 266.725 kb on + strand, within MMJJ_RS01375 at 266.764 kb on - strand, within MMJJ_RS01375 at 266.764 kb on - strand, within MMJJ_RS01375 at 266.767 kb on + strand, within MMJJ_RS01375 at 266.779 kb on - strand at 266.779 kb on - strand at 266.779 kb on - strand at 266.780 kb on + strand at 266.857 kb on + strand at 266.857 kb on + strand at 266.859 kb on - strand at 266.868 kb on + strand at 266.888 kb on - strand at 266.889 kb on + strand at 266.889 kb on + strand at 267.043 kb on + strand, within MMJJ_RS01380 at 267.046 kb on - strand, within MMJJ_RS01380 at 267.046 kb on - strand, within MMJJ_RS01380 at 267.047 kb on + strand, within MMJJ_RS01380 at 267.070 kb on - strand, within MMJJ_RS01380 at 267.077 kb on - strand, within MMJJ_RS01380 at 267.077 kb on - strand, within MMJJ_RS01380 at 267.077 kb on - strand, within MMJJ_RS01380 at 267.078 kb on + strand, within MMJJ_RS01380 at 267.078 kb on + strand, within MMJJ_RS01380 at 267.078 kb on + strand, within MMJJ_RS01380 at 267.078 kb on + strand, within MMJJ_RS01380 at 267.078 kb on + strand, within MMJJ_RS01380 at 267.082 kb on + strand, within MMJJ_RS01380 at 267.082 kb on + strand, within MMJJ_RS01380 at 267.180 kb on + strand, within MMJJ_RS01380 at 267.180 kb on + strand, within MMJJ_RS01380 at 267.182 kb on - strand, within MMJJ_RS01380 at 267.183 kb on + strand, within MMJJ_RS01380 at 267.242 kb on + strand, within MMJJ_RS01380 at 267.267 kb on - strand, within MMJJ_RS01380 at 267.267 kb on - strand, within MMJJ_RS01380 at 267.354 kb on - strand at 267.354 kb on - strand at 267.355 kb on + strand at 267.355 kb on + strand at 267.359 kb on - strand at 267.365 kb on - strand at 267.365 kb on - strand at 267.366 kb on + strand at 267.366 kb on + strand at 267.367 kb on - strand at 267.368 kb on + strand at 267.389 kb on + strand at 267.416 kb on - strand at 267.423 kb on - strand at 267.445 kb on - strand at 267.446 kb on + strand at 267.446 kb on + strand at 267.450 kb on + strand at 267.457 kb on - strand at 267.464 kb on + strand at 267.476 kb on + strand at 267.477 kb on - strand at 267.477 kb on - strand at 267.477 kb on - strand at 267.477 kb on - strand at 267.478 kb on + strand at 267.478 kb on + strand at 267.478 kb on + strand at 267.479 kb on - strand at 267.479 kb on - strand at 267.479 kb on - strand at 267.480 kb on + strand at 267.481 kb on - strand at 267.502 kb on + strand at 267.502 kb on + strand at 267.519 kb on - strand at 267.519 kb on - strand at 267.519 kb on - strand at 267.519 kb on - strand at 267.562 kb on - strand at 267.562 kb on - strand at 267.603 kb on - strand, within MMJJ_RS01385 at 267.603 kb on - strand, within MMJJ_RS01385 at 267.603 kb on - strand, within MMJJ_RS01385 at 267.604 kb on + strand, within MMJJ_RS01385 at 267.604 kb on + strand, within MMJJ_RS01385 at 267.604 kb on + strand, within MMJJ_RS01385 at 267.604 kb on + strand, within MMJJ_RS01385 at 267.604 kb on + strand, within MMJJ_RS01385 at 267.604 kb on + strand, within MMJJ_RS01385 at 267.604 kb on + strand, within MMJJ_RS01385 at 267.676 kb on - strand, within MMJJ_RS01385 at 267.678 kb on - strand, within MMJJ_RS01385 at 267.678 kb on - strand, within MMJJ_RS01385 at 267.678 kb on - strand, within MMJJ_RS01385 at 267.678 kb on - strand, within MMJJ_RS01385 at 267.678 kb on - strand, within MMJJ_RS01385 at 267.678 kb on - strand, within MMJJ_RS01385 at 267.678 kb on - strand, within MMJJ_RS01385 at 267.678 kb on - strand, within MMJJ_RS01385 at 267.678 kb on - strand, within MMJJ_RS01385 at 267.679 kb on + strand, within MMJJ_RS01385 at 267.679 kb on + strand, within MMJJ_RS01385 at 267.679 kb on + strand at 267.679 kb on + strand, within MMJJ_RS01385 at 267.679 kb on + strand, within MMJJ_RS01385 at 267.679 kb on + strand, within MMJJ_RS01385 at 267.681 kb on - strand, within MMJJ_RS01385 at 267.681 kb on - strand, within MMJJ_RS01385 at 267.681 kb on - strand, within MMJJ_RS01385 at 267.681 kb on - strand, within MMJJ_RS01385 at 267.681 kb on - strand, within MMJJ_RS01385 at 267.681 kb on - strand, within MMJJ_RS01385 at 267.681 kb on - strand, within MMJJ_RS01385 at 267.681 kb on - strand, within MMJJ_RS01385 at 267.682 kb on + strand, within MMJJ_RS01385 at 267.682 kb on + strand, within MMJJ_RS01385 at 267.682 kb on + strand, within MMJJ_RS01385 at 267.682 kb on + strand, within MMJJ_RS01385 at 267.689 kb on + strand, within MMJJ_RS01385 at 267.760 kb on + strand, within MMJJ_RS01385 at 267.944 kb on + strand, within MMJJ_RS01385 at 267.944 kb on + strand, within MMJJ_RS01385 at 267.946 kb on + strand, within MMJJ_RS01385 at 268.131 kb on - strand at 268.159 kb on + strand at 268.159 kb on + strand at 268.214 kb on + strand at 268.214 kb on + strand at 268.215 kb on - strand at 268.261 kb on - strand, within MMJJ_RS01390 at 268.262 kb on + strand, within MMJJ_RS01390 at 268.314 kb on - strand, within MMJJ_RS01390
Per-strain Table
Position Strand Gene LocusTag Fraction H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 FREE remove 265,913 + -0.9 265,951 + +0.2 265,979 - -0.5 266,032 - +0.0 266,033 + +0.2 266,078 + MMJJ_RS01375 0.11 -0.3 266,114 + MMJJ_RS01375 0.15 +0.4 266,114 + MMJJ_RS01375 0.15 +1.5 266,119 - MMJJ_RS01375 0.16 -0.5 266,119 - MMJJ_RS01375 0.16 +0.3 266,119 - MMJJ_RS01375 0.16 +0.6 266,120 + MMJJ_RS01375 0.16 -0.1 266,174 + MMJJ_RS01375 0.22 +0.1 266,247 - MMJJ_RS01375 0.31 -0.7 266,250 + MMJJ_RS01375 0.31 +0.1 266,336 - MMJJ_RS01375 0.41 +0.6 266,337 + MMJJ_RS01375 0.41 -0.6 266,337 + MMJJ_RS01375 0.41 +0.9 266,358 - MMJJ_RS01375 0.43 -0.4 266,398 + MMJJ_RS01375 0.48 -0.2 266,410 - MMJJ_RS01375 0.49 +0.7 266,411 + MMJJ_RS01375 0.49 +0.4 266,411 + MMJJ_RS01375 0.49 -0.3 266,447 + MMJJ_RS01375 0.53 +0.1 266,466 - MMJJ_RS01375 0.56 +1.5 266,562 - MMJJ_RS01375 0.66 +0.1 266,599 - MMJJ_RS01375 0.71 -0.3 266,599 - MMJJ_RS01375 0.71 -0.5 266,600 + MMJJ_RS01375 0.71 -0.7 266,605 - MMJJ_RS01375 0.71 +0.7 266,605 - MMJJ_RS01375 0.71 +0.6 266,605 - MMJJ_RS01375 0.71 -0.3 266,605 - MMJJ_RS01375 0.71 -0.4 266,609 - MMJJ_RS01375 0.72 -0.3 266,621 - MMJJ_RS01375 0.73 -0.5 266,621 - MMJJ_RS01375 0.73 -0.9 266,621 - MMJJ_RS01375 0.73 -0.2 266,626 - MMJJ_RS01375 0.74 +0.1 266,627 + MMJJ_RS01375 0.74 +1.3 266,627 + MMJJ_RS01375 0.74 -0.4 266,674 + MMJJ_RS01375 0.79 -0.1 266,706 - MMJJ_RS01375 0.83 +0.6 266,725 + MMJJ_RS01375 0.85 -1.4 266,764 - MMJJ_RS01375 0.89 -1.5 266,764 - MMJJ_RS01375 0.89 -0.1 266,767 + MMJJ_RS01375 0.90 -0.2 266,779 - -0.5 266,779 - -0.4 266,779 - +0.2 266,780 + +0.3 266,857 + -0.6 266,857 + -0.7 266,859 - +1.1 266,868 + -0.1 266,888 - -0.7 266,889 + +0.4 266,889 + -0.8 267,043 + MMJJ_RS01380 0.33 -0.6 267,046 - MMJJ_RS01380 0.34 -0.7 267,046 - MMJJ_RS01380 0.34 +0.1 267,047 + MMJJ_RS01380 0.34 +0.2 267,070 - MMJJ_RS01380 0.39 +0.0 267,077 - MMJJ_RS01380 0.41 +0.7 267,077 - MMJJ_RS01380 0.41 +0.1 267,077 - MMJJ_RS01380 0.41 -0.0 267,078 + MMJJ_RS01380 0.41 -0.1 267,078 + MMJJ_RS01380 0.41 +1.0 267,078 + MMJJ_RS01380 0.41 -0.4 267,078 + MMJJ_RS01380 0.41 +0.3 267,078 + MMJJ_RS01380 0.41 -0.1 267,082 + MMJJ_RS01380 0.42 +1.5 267,082 + MMJJ_RS01380 0.42 -0.3 267,180 + MMJJ_RS01380 0.64 -0.2 267,180 + MMJJ_RS01380 0.64 +0.1 267,182 - MMJJ_RS01380 0.64 +1.5 267,183 + MMJJ_RS01380 0.65 +0.7 267,242 + MMJJ_RS01380 0.78 +1.5 267,267 - MMJJ_RS01380 0.84 +0.4 267,267 - MMJJ_RS01380 0.84 +0.1 267,354 - -0.3 267,354 - +0.3 267,355 + +0.1 267,355 + -0.4 267,359 - +1.7 267,365 - +0.1 267,365 - +0.6 267,366 + -0.5 267,366 + -1.1 267,367 - -0.9 267,368 + +0.7 267,389 + -0.7 267,416 - +0.5 267,423 - -0.9 267,445 - -0.8 267,446 + +1.9 267,446 + -0.0 267,450 + +0.1 267,457 - +0.2 267,464 + +0.0 267,476 + +0.7 267,477 - +0.9 267,477 - -0.5 267,477 - +1.1 267,477 - +0.3 267,478 + -0.5 267,478 + +0.4 267,478 + -0.6 267,479 - +0.9 267,479 - +0.1 267,479 - +0.5 267,480 + +1.4 267,481 - +0.0 267,502 + -0.6 267,502 + -1.2 267,519 - -0.2 267,519 - +1.8 267,519 - +0.6 267,519 - -0.5 267,562 - -0.3 267,562 - +1.1 267,603 - MMJJ_RS01385 0.12 +0.3 267,603 - MMJJ_RS01385 0.12 -0.6 267,603 - MMJJ_RS01385 0.12 +0.5 267,604 + MMJJ_RS01385 0.13 -0.2 267,604 + MMJJ_RS01385 0.13 -0.3 267,604 + MMJJ_RS01385 0.13 +0.3 267,604 + MMJJ_RS01385 0.13 +1.0 267,604 + MMJJ_RS01385 0.13 -1.6 267,604 + MMJJ_RS01385 0.13 -0.0 267,604 + MMJJ_RS01385 0.13 -0.5 267,676 - MMJJ_RS01385 0.26 -1.0 267,678 - MMJJ_RS01385 0.26 +0.6 267,678 - MMJJ_RS01385 0.26 +0.3 267,678 - MMJJ_RS01385 0.26 +0.3 267,678 - MMJJ_RS01385 0.26 -1.3 267,678 - MMJJ_RS01385 0.26 -0.8 267,678 - MMJJ_RS01385 0.26 -0.1 267,678 - MMJJ_RS01385 0.26 +0.5 267,678 - MMJJ_RS01385 0.26 +0.9 267,678 - MMJJ_RS01385 0.26 +0.1 267,679 + MMJJ_RS01385 0.26 -0.2 267,679 + MMJJ_RS01385 0.26 -0.2 267,679 + -0.9 267,679 + MMJJ_RS01385 0.26 -0.9 267,679 + MMJJ_RS01385 0.26 -0.5 267,679 + MMJJ_RS01385 0.26 -0.2 267,681 - MMJJ_RS01385 0.27 +1.3 267,681 - MMJJ_RS01385 0.27 -0.2 267,681 - MMJJ_RS01385 0.27 -0.4 267,681 - MMJJ_RS01385 0.27 -0.9 267,681 - MMJJ_RS01385 0.27 +0.2 267,681 - MMJJ_RS01385 0.27 +0.7 267,681 - MMJJ_RS01385 0.27 +0.8 267,681 - MMJJ_RS01385 0.27 -0.9 267,682 + MMJJ_RS01385 0.27 +0.5 267,682 + MMJJ_RS01385 0.27 +1.8 267,682 + MMJJ_RS01385 0.27 -0.6 267,682 + MMJJ_RS01385 0.27 -0.5 267,689 + MMJJ_RS01385 0.28 +1.2 267,760 + MMJJ_RS01385 0.41 -0.7 267,944 + MMJJ_RS01385 0.74 -1.1 267,944 + MMJJ_RS01385 0.74 +3.1 267,946 + MMJJ_RS01385 0.75 -0.4 268,131 - -0.0 268,159 + +1.1 268,159 + -0.9 268,214 + -0.6 268,214 + +1.5 268,215 - -0.6 268,261 - MMJJ_RS01390 0.11 +1.4 268,262 + MMJJ_RS01390 0.11 +0.4 268,314 - MMJJ_RS01390 0.15 -0.1
Or see this region's nucleotide sequence