Experiment: H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 FREE
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS00130 and MMJJ_RS00135 are separated by 86 nucleotides MMJJ_RS00135 and MMJJ_RS00140 are separated by 48 nucleotides MMJJ_RS00140 and MMJJ_RS00145 are separated by 125 nucleotides
MMJJ_RS00130: MMJJ_RS00130 - uroporphyrinogen-III C-methyltransferase, at 24,902 to 25,621
_RS00130
MMJJ_RS00135: MMJJ_RS00135 - FmdE family protein, at 25,708 to 26,286
_RS00135
MMJJ_RS00140: MMJJ_RS00140 - hypothetical protein, at 26,335 to 26,592
_RS00140
MMJJ_RS00145: MMJJ_RS00145 - aquaporin family protein, at 26,718 to 27,437
_RS00145
Position (kb) 25
26
27 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 24.714 kb on + strand at 24.725 kb on - strand at 24.726 kb on + strand at 24.743 kb on - strand at 24.744 kb on + strand at 24.745 kb on - strand at 24.745 kb on - strand at 24.749 kb on - strand at 24.753 kb on - strand at 24.754 kb on + strand at 24.779 kb on + strand at 24.796 kb on + strand at 24.817 kb on + strand at 24.829 kb on + strand at 24.833 kb on + strand at 24.855 kb on - strand at 24.855 kb on - strand at 24.856 kb on + strand at 24.856 kb on + strand at 24.861 kb on + strand at 24.861 kb on + strand at 24.861 kb on + strand at 25.147 kb on + strand, within MMJJ_RS00130 at 25.221 kb on - strand, within MMJJ_RS00130 at 25.246 kb on - strand, within MMJJ_RS00130 at 25.615 kb on + strand at 25.652 kb on - strand at 25.653 kb on + strand at 25.653 kb on + strand at 25.653 kb on + strand at 25.673 kb on - strand at 25.673 kb on - strand at 25.685 kb on - strand at 25.686 kb on + strand at 25.789 kb on - strand, within MMJJ_RS00135 at 25.789 kb on - strand, within MMJJ_RS00135 at 25.796 kb on - strand, within MMJJ_RS00135 at 25.804 kb on - strand, within MMJJ_RS00135 at 25.804 kb on - strand, within MMJJ_RS00135 at 25.805 kb on + strand, within MMJJ_RS00135 at 25.849 kb on + strand, within MMJJ_RS00135 at 25.860 kb on - strand, within MMJJ_RS00135 at 25.860 kb on - strand, within MMJJ_RS00135 at 25.871 kb on - strand, within MMJJ_RS00135 at 25.939 kb on - strand, within MMJJ_RS00135 at 25.991 kb on + strand, within MMJJ_RS00135 at 26.009 kb on - strand, within MMJJ_RS00135 at 26.009 kb on - strand, within MMJJ_RS00135 at 26.136 kb on + strand, within MMJJ_RS00135 at 26.160 kb on - strand, within MMJJ_RS00135 at 26.187 kb on - strand, within MMJJ_RS00135 at 26.187 kb on - strand, within MMJJ_RS00135 at 26.187 kb on - strand, within MMJJ_RS00135 at 26.187 kb on - strand, within MMJJ_RS00135 at 26.188 kb on + strand, within MMJJ_RS00135 at 26.215 kb on + strand, within MMJJ_RS00135 at 26.235 kb on - strand at 26.235 kb on - strand at 26.249 kb on - strand at 26.250 kb on + strand at 26.257 kb on + strand at 26.289 kb on + strand at 26.353 kb on - strand at 26.353 kb on - strand at 26.357 kb on - strand at 26.357 kb on - strand at 26.357 kb on - strand at 26.358 kb on + strand at 26.361 kb on - strand, within MMJJ_RS00140 at 26.398 kb on - strand, within MMJJ_RS00140 at 26.399 kb on + strand, within MMJJ_RS00140 at 26.399 kb on + strand, within MMJJ_RS00140 at 26.400 kb on - strand, within MMJJ_RS00140 at 26.401 kb on + strand, within MMJJ_RS00140 at 26.401 kb on + strand, within MMJJ_RS00140 at 26.401 kb on + strand, within MMJJ_RS00140 at 26.402 kb on - strand, within MMJJ_RS00140 at 26.403 kb on + strand, within MMJJ_RS00140 at 26.407 kb on - strand, within MMJJ_RS00140 at 26.408 kb on + strand, within MMJJ_RS00140 at 26.412 kb on - strand, within MMJJ_RS00140 at 26.574 kb on + strand at 26.583 kb on - strand at 26.604 kb on - strand at 26.609 kb on + strand at 26.609 kb on + strand at 26.609 kb on + strand at 26.611 kb on - strand at 26.614 kb on - strand at 26.614 kb on - strand at 26.615 kb on + strand at 26.619 kb on + strand at 26.730 kb on - strand at 26.730 kb on - strand at 26.730 kb on - strand at 26.740 kb on - strand at 26.740 kb on - strand at 26.741 kb on + strand at 26.744 kb on - strand at 26.744 kb on - strand at 26.744 kb on - strand at 26.745 kb on + strand at 26.757 kb on - strand at 26.757 kb on - strand at 26.757 kb on - strand at 26.757 kb on - strand at 26.758 kb on + strand at 26.783 kb on - strand at 26.783 kb on - strand at 26.783 kb on - strand at 26.783 kb on - strand at 26.784 kb on + strand at 26.784 kb on + strand at 26.784 kb on + strand at 26.785 kb on - strand at 26.785 kb on - strand at 26.785 kb on - strand at 26.785 kb on - strand at 26.785 kb on - strand at 26.785 kb on - strand at 26.786 kb on + strand at 26.786 kb on + strand at 26.786 kb on + strand at 26.786 kb on + strand at 26.786 kb on + strand at 26.786 kb on + strand at 26.786 kb on + strand at 26.788 kb on + strand at 26.797 kb on - strand, within MMJJ_RS00145 at 26.797 kb on - strand, within MMJJ_RS00145 at 26.797 kb on - strand, within MMJJ_RS00145 at 26.801 kb on + strand, within MMJJ_RS00145 at 26.801 kb on + strand, within MMJJ_RS00145 at 26.807 kb on - strand, within MMJJ_RS00145 at 26.822 kb on - strand, within MMJJ_RS00145 at 26.823 kb on + strand, within MMJJ_RS00145 at 26.823 kb on + strand, within MMJJ_RS00145 at 26.825 kb on - strand, within MMJJ_RS00145 at 26.825 kb on - strand, within MMJJ_RS00145 at 26.825 kb on - strand, within MMJJ_RS00145 at 26.826 kb on + strand, within MMJJ_RS00145 at 26.826 kb on + strand, within MMJJ_RS00145 at 26.826 kb on + strand, within MMJJ_RS00145 at 26.826 kb on + strand, within MMJJ_RS00145 at 26.826 kb on + strand, within MMJJ_RS00145 at 26.829 kb on - strand, within MMJJ_RS00145 at 26.830 kb on + strand, within MMJJ_RS00145 at 26.863 kb on + strand, within MMJJ_RS00145 at 26.880 kb on + strand, within MMJJ_RS00145 at 26.973 kb on - strand, within MMJJ_RS00145 at 26.975 kb on - strand, within MMJJ_RS00145 at 27.003 kb on + strand, within MMJJ_RS00145 at 27.075 kb on - strand, within MMJJ_RS00145 at 27.108 kb on - strand, within MMJJ_RS00145 at 27.133 kb on + strand, within MMJJ_RS00145 at 27.146 kb on - strand, within MMJJ_RS00145 at 27.146 kb on - strand, within MMJJ_RS00145 at 27.147 kb on + strand, within MMJJ_RS00145 at 27.147 kb on + strand, within MMJJ_RS00145 at 27.148 kb on - strand, within MMJJ_RS00145 at 27.149 kb on + strand, within MMJJ_RS00145 at 27.150 kb on - strand, within MMJJ_RS00145 at 27.150 kb on - strand, within MMJJ_RS00145 at 27.150 kb on - strand, within MMJJ_RS00145 at 27.150 kb on - strand, within MMJJ_RS00145 at 27.150 kb on - strand, within MMJJ_RS00145 at 27.151 kb on + strand, within MMJJ_RS00145 at 27.151 kb on + strand, within MMJJ_RS00145 at 27.151 kb on + strand, within MMJJ_RS00145 at 27.151 kb on + strand, within MMJJ_RS00145 at 27.159 kb on + strand, within MMJJ_RS00145 at 27.200 kb on - strand, within MMJJ_RS00145 at 27.201 kb on + strand, within MMJJ_RS00145 at 27.213 kb on + strand, within MMJJ_RS00145 at 27.230 kb on - strand, within MMJJ_RS00145 at 27.230 kb on - strand, within MMJJ_RS00145 at 27.242 kb on - strand, within MMJJ_RS00145 at 27.242 kb on - strand, within MMJJ_RS00145 at 27.242 kb on - strand, within MMJJ_RS00145 at 27.249 kb on - strand, within MMJJ_RS00145 at 27.250 kb on + strand, within MMJJ_RS00145
Per-strain Table
Position Strand Gene LocusTag Fraction H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 FREE remove 24,714 + -2.2 24,725 - -3.2 24,726 + -1.2 24,743 - -1.7 24,744 + -2.6 24,745 - -2.6 24,745 - -3.3 24,749 - -2.2 24,753 - +1.9 24,754 + +0.4 24,779 + -0.2 24,796 + -2.0 24,817 + +0.6 24,829 + -1.8 24,833 + -2.4 24,855 - -2.6 24,855 - -2.6 24,856 + -2.7 24,856 + -1.5 24,861 + -1.7 24,861 + -3.5 24,861 + -2.9 25,147 + MMJJ_RS00130 0.34 -0.9 25,221 - MMJJ_RS00130 0.44 -3.1 25,246 - MMJJ_RS00130 0.48 -2.6 25,615 + +0.6 25,652 - -0.9 25,653 + +0.2 25,653 + +1.0 25,653 + -0.9 25,673 - +0.3 25,673 - +0.2 25,685 - +1.0 25,686 + -0.5 25,789 - MMJJ_RS00135 0.14 -0.6 25,789 - MMJJ_RS00135 0.14 -0.1 25,796 - MMJJ_RS00135 0.15 -1.9 25,804 - MMJJ_RS00135 0.17 +0.3 25,804 - MMJJ_RS00135 0.17 +1.6 25,805 + MMJJ_RS00135 0.17 +0.3 25,849 + MMJJ_RS00135 0.24 +1.3 25,860 - MMJJ_RS00135 0.26 -0.5 25,860 - MMJJ_RS00135 0.26 +0.8 25,871 - MMJJ_RS00135 0.28 -0.2 25,939 - MMJJ_RS00135 0.40 +0.6 25,991 + MMJJ_RS00135 0.49 -0.5 26,009 - MMJJ_RS00135 0.52 -0.4 26,009 - MMJJ_RS00135 0.52 -1.5 26,136 + MMJJ_RS00135 0.74 +1.4 26,160 - MMJJ_RS00135 0.78 -1.0 26,187 - MMJJ_RS00135 0.83 +1.0 26,187 - MMJJ_RS00135 0.83 +0.5 26,187 - MMJJ_RS00135 0.83 +0.3 26,187 - MMJJ_RS00135 0.83 +0.7 26,188 + MMJJ_RS00135 0.83 +0.6 26,215 + MMJJ_RS00135 0.88 -0.3 26,235 - +0.5 26,235 - -1.1 26,249 - +1.4 26,250 + +0.5 26,257 + -0.4 26,289 + -1.1 26,353 - +0.0 26,353 - -0.6 26,357 - +0.0 26,357 - -0.5 26,357 - +1.7 26,358 + +0.8 26,361 - MMJJ_RS00140 0.10 -0.4 26,398 - MMJJ_RS00140 0.24 +1.3 26,399 + MMJJ_RS00140 0.25 +0.0 26,399 + MMJJ_RS00140 0.25 +0.5 26,400 - MMJJ_RS00140 0.25 -0.2 26,401 + MMJJ_RS00140 0.26 +0.2 26,401 + MMJJ_RS00140 0.26 +0.5 26,401 + MMJJ_RS00140 0.26 -0.1 26,402 - MMJJ_RS00140 0.26 +0.7 26,403 + MMJJ_RS00140 0.26 +0.6 26,407 - MMJJ_RS00140 0.28 +1.1 26,408 + MMJJ_RS00140 0.28 +0.0 26,412 - MMJJ_RS00140 0.30 -0.3 26,574 + -1.3 26,583 - +0.8 26,604 - +1.0 26,609 + -0.3 26,609 + -0.9 26,609 + -0.0 26,611 - +0.1 26,614 - +0.1 26,614 - +0.1 26,615 + +1.7 26,619 + -0.4 26,730 - +0.1 26,730 - -0.2 26,730 - -0.2 26,740 - -0.3 26,740 - -1.6 26,741 + -1.7 26,744 - +0.2 26,744 - +0.1 26,744 - -0.8 26,745 + +0.3 26,757 - +1.2 26,757 - -0.3 26,757 - +1.2 26,757 - +0.0 26,758 + -0.1 26,783 - -1.0 26,783 - -0.6 26,783 - -0.5 26,783 - +0.6 26,784 + +0.8 26,784 + -0.7 26,784 + +1.1 26,785 - +0.3 26,785 - -0.4 26,785 - -0.5 26,785 - +0.8 26,785 - -1.0 26,785 - -0.6 26,786 + -0.6 26,786 + -0.2 26,786 + +0.7 26,786 + -0.9 26,786 + +0.5 26,786 + +0.4 26,786 + -0.6 26,788 + -0.2 26,797 - MMJJ_RS00145 0.11 +0.0 26,797 - MMJJ_RS00145 0.11 +0.0 26,797 - MMJJ_RS00145 0.11 +0.3 26,801 + MMJJ_RS00145 0.12 -0.6 26,801 + MMJJ_RS00145 0.12 -0.3 26,807 - MMJJ_RS00145 0.12 -0.3 26,822 - MMJJ_RS00145 0.14 -2.2 26,823 + MMJJ_RS00145 0.15 +1.4 26,823 + MMJJ_RS00145 0.15 -0.1 26,825 - MMJJ_RS00145 0.15 +2.3 26,825 - MMJJ_RS00145 0.15 +0.9 26,825 - MMJJ_RS00145 0.15 +0.6 26,826 + MMJJ_RS00145 0.15 +1.7 26,826 + MMJJ_RS00145 0.15 -0.7 26,826 + MMJJ_RS00145 0.15 +0.9 26,826 + MMJJ_RS00145 0.15 -0.5 26,826 + MMJJ_RS00145 0.15 +0.6 26,829 - MMJJ_RS00145 0.15 -0.6 26,830 + MMJJ_RS00145 0.16 +0.6 26,863 + MMJJ_RS00145 0.20 -0.5 26,880 + MMJJ_RS00145 0.23 -0.3 26,973 - MMJJ_RS00145 0.35 +0.5 26,975 - MMJJ_RS00145 0.36 +0.8 27,003 + MMJJ_RS00145 0.40 +0.5 27,075 - MMJJ_RS00145 0.50 +2.1 27,108 - MMJJ_RS00145 0.54 -1.4 27,133 + MMJJ_RS00145 0.58 -1.4 27,146 - MMJJ_RS00145 0.59 +0.3 27,146 - MMJJ_RS00145 0.59 +0.0 27,147 + MMJJ_RS00145 0.60 -0.5 27,147 + MMJJ_RS00145 0.60 +0.4 27,148 - MMJJ_RS00145 0.60 -2.8 27,149 + MMJJ_RS00145 0.60 -0.6 27,150 - MMJJ_RS00145 0.60 -0.1 27,150 - MMJJ_RS00145 0.60 +0.1 27,150 - MMJJ_RS00145 0.60 -0.0 27,150 - MMJJ_RS00145 0.60 -0.5 27,150 - MMJJ_RS00145 0.60 -0.3 27,151 + MMJJ_RS00145 0.60 +0.1 27,151 + MMJJ_RS00145 0.60 -0.7 27,151 + MMJJ_RS00145 0.60 +1.0 27,151 + MMJJ_RS00145 0.60 -0.8 27,159 + MMJJ_RS00145 0.61 -0.7 27,200 - MMJJ_RS00145 0.67 +0.6 27,201 + MMJJ_RS00145 0.67 +0.2 27,213 + MMJJ_RS00145 0.69 -0.9 27,230 - MMJJ_RS00145 0.71 +0.4 27,230 - MMJJ_RS00145 0.71 +0.3 27,242 - MMJJ_RS00145 0.73 +1.0 27,242 - MMJJ_RS00145 0.73 -0.4 27,242 - MMJJ_RS00145 0.73 +0.2 27,249 - MMJJ_RS00145 0.74 +0.7 27,250 + MMJJ_RS00145 0.74 +0.1
Or see this region's nucleotide sequence