Experiment: R2A with nitrite 50 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK54_RS06510 and LRK54_RS06515 are separated by 16 nucleotides LRK54_RS06515 and LRK54_RS06520 overlap by 4 nucleotides LRK54_RS06520 and LRK54_RS06525 are separated by 12 nucleotides
LRK54_RS06510: LRK54_RS06510 - penicillin-binding protein 2, at 1,399,098 to 1,401,104
_RS06510
LRK54_RS06515: LRK54_RS06515 - rod shape-determining protein MreD, at 1,401,121 to 1,401,606
_RS06515
LRK54_RS06520: LRK54_RS06520 - rod shape-determining protein MreC, at 1,401,603 to 1,402,553
_RS06520
LRK54_RS06525: LRK54_RS06525 - rod shape-determining protein, at 1,402,566 to 1,403,612
_RS06525
Position (kb)
1401
1402 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1401.178 kb on + strand, within LRK54_RS06515 at 1401.178 kb on + strand, within LRK54_RS06515 at 1401.179 kb on - strand, within LRK54_RS06515 at 1401.179 kb on - strand, within LRK54_RS06515 at 1401.179 kb on - strand, within LRK54_RS06515 at 1401.179 kb on - strand, within LRK54_RS06515 at 1401.179 kb on - strand, within LRK54_RS06515 at 1401.179 kb on - strand, within LRK54_RS06515 at 1401.179 kb on - strand, within LRK54_RS06515 at 1401.179 kb on - strand, within LRK54_RS06515 at 1401.179 kb on - strand, within LRK54_RS06515 at 1401.179 kb on - strand, within LRK54_RS06515 at 1401.179 kb on - strand, within LRK54_RS06515 at 1401.179 kb on - strand, within LRK54_RS06515 at 1402.568 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with nitrite 50 mM remove 1,401,178 + LRK54_RS06515 0.12 -3.0 1,401,178 + LRK54_RS06515 0.12 -0.3 1,401,179 - LRK54_RS06515 0.12 -2.5 1,401,179 - LRK54_RS06515 0.12 -0.9 1,401,179 - LRK54_RS06515 0.12 -1.0 1,401,179 - LRK54_RS06515 0.12 -1.8 1,401,179 - LRK54_RS06515 0.12 -3.2 1,401,179 - LRK54_RS06515 0.12 +0.3 1,401,179 - LRK54_RS06515 0.12 -1.8 1,401,179 - LRK54_RS06515 0.12 -1.6 1,401,179 - LRK54_RS06515 0.12 -0.5 1,401,179 - LRK54_RS06515 0.12 -0.9 1,401,179 - LRK54_RS06515 0.12 -1.3 1,401,179 - LRK54_RS06515 0.12 -1.2 1,402,568 - +0.4
Or see this region's nucleotide sequence