Experiment: R2A with nitrite 50 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK54_RS00400 and LRK54_RS00405 are separated by 67 nucleotides LRK54_RS00405 and LRK54_RS00410 are separated by 156 nucleotides LRK54_RS00410 and LRK54_RS00415 are separated by 107 nucleotides LRK54_RS00415 and LRK54_RS00420 are separated by 113 nucleotides
LRK54_RS00400: LRK54_RS00400 - 3-phosphoserine/phosphohydroxythreonine transaminase, at 87,223 to 88,311
_RS00400
LRK54_RS00405: LRK54_RS00405 - FHA domain-containing protein, at 88,379 to 89,140
_RS00405
LRK54_RS00410: LRK54_RS00410 - polyhydroxyalkanoic acid system family protein, at 89,297 to 89,572
_RS00410
LRK54_RS00415: LRK54_RS00415 - translation initiation factor IF-1, at 89,680 to 89,898
_RS00415
LRK54_RS00420: LRK54_RS00420 - ATP-dependent Clp protease ATP-binding subunit ClpA, at 90,012 to 92,282
_RS00420
Position (kb) 89
90 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 88.347 kb on + strand at 88.347 kb on + strand at 88.348 kb on - strand at 88.348 kb on - strand at 88.348 kb on - strand at 88.767 kb on - strand, within LRK54_RS00405 at 88.953 kb on + strand, within LRK54_RS00405 at 88.953 kb on + strand, within LRK54_RS00405 at 88.953 kb on + strand, within LRK54_RS00405 at 88.953 kb on + strand, within LRK54_RS00405 at 88.953 kb on + strand, within LRK54_RS00405 at 88.953 kb on + strand, within LRK54_RS00405 at 88.953 kb on + strand, within LRK54_RS00405 at 88.953 kb on + strand, within LRK54_RS00405 at 88.953 kb on + strand, within LRK54_RS00405 at 88.953 kb on + strand, within LRK54_RS00405 at 88.953 kb on + strand, within LRK54_RS00405 at 88.953 kb on + strand, within LRK54_RS00405 at 88.953 kb on + strand, within LRK54_RS00405 at 88.953 kb on + strand, within LRK54_RS00405 at 88.953 kb on + strand, within LRK54_RS00405 at 88.953 kb on + strand, within LRK54_RS00405 at 88.953 kb on + strand, within LRK54_RS00405 at 88.954 kb on - strand, within LRK54_RS00405 at 88.954 kb on - strand, within LRK54_RS00405 at 88.954 kb on - strand, within LRK54_RS00405 at 88.954 kb on - strand, within LRK54_RS00405 at 88.954 kb on - strand, within LRK54_RS00405 at 88.954 kb on - strand, within LRK54_RS00405 at 88.954 kb on - strand, within LRK54_RS00405 at 88.954 kb on - strand, within LRK54_RS00405 at 88.954 kb on - strand, within LRK54_RS00405 at 88.954 kb on - strand, within LRK54_RS00405 at 88.954 kb on - strand, within LRK54_RS00405 at 88.954 kb on - strand, within LRK54_RS00405 at 88.954 kb on - strand, within LRK54_RS00405 at 88.954 kb on - strand, within LRK54_RS00405 at 88.954 kb on - strand, within LRK54_RS00405 at 88.954 kb on - strand, within LRK54_RS00405 at 88.954 kb on - strand, within LRK54_RS00405 at 88.954 kb on - strand, within LRK54_RS00405 at 88.954 kb on - strand, within LRK54_RS00405 at 88.954 kb on - strand, within LRK54_RS00405 at 89.012 kb on - strand, within LRK54_RS00405 at 89.071 kb on + strand at 89.071 kb on + strand at 89.134 kb on + strand at 89.135 kb on - strand at 89.135 kb on - strand at 89.171 kb on + strand at 89.171 kb on + strand at 89.171 kb on + strand at 89.171 kb on + strand at 89.171 kb on + strand at 89.171 kb on + strand at 89.171 kb on + strand at 89.171 kb on + strand at 89.171 kb on + strand at 89.171 kb on + strand at 89.171 kb on + strand at 89.171 kb on + strand at 89.172 kb on - strand at 89.172 kb on - strand at 89.172 kb on - strand at 89.172 kb on - strand at 89.172 kb on - strand at 89.172 kb on - strand at 89.172 kb on - strand at 89.172 kb on - strand at 89.172 kb on - strand at 89.172 kb on - strand at 89.172 kb on - strand at 89.172 kb on - strand at 89.172 kb on - strand at 89.172 kb on - strand at 89.172 kb on - strand at 89.172 kb on - strand at 89.172 kb on - strand at 89.172 kb on - strand at 89.172 kb on - strand at 89.172 kb on - strand at 89.172 kb on - strand at 89.172 kb on - strand at 89.267 kb on + strand at 89.267 kb on + strand at 89.267 kb on + strand at 89.267 kb on + strand at 89.267 kb on + strand at 89.267 kb on + strand at 89.267 kb on + strand at 89.267 kb on + strand at 89.267 kb on + strand at 89.267 kb on + strand at 89.267 kb on + strand at 89.267 kb on + strand at 89.267 kb on + strand at 89.267 kb on + strand at 89.267 kb on + strand at 89.267 kb on + strand at 89.267 kb on + strand at 89.268 kb on - strand at 89.268 kb on - strand at 89.268 kb on - strand at 89.268 kb on - strand at 89.268 kb on - strand at 89.268 kb on - strand at 89.268 kb on - strand at 89.268 kb on - strand at 89.268 kb on - strand at 89.268 kb on - strand at 89.268 kb on - strand at 89.268 kb on - strand at 89.268 kb on - strand at 89.268 kb on - strand at 89.268 kb on - strand at 89.268 kb on - strand at 89.268 kb on - strand at 89.268 kb on - strand at 89.268 kb on - strand at 89.268 kb on - strand at 89.268 kb on - strand at 89.282 kb on - strand at 89.282 kb on - strand at 89.282 kb on - strand at 89.355 kb on + strand, within LRK54_RS00410 at 89.355 kb on + strand, within LRK54_RS00410 at 89.356 kb on - strand, within LRK54_RS00410 at 89.356 kb on - strand, within LRK54_RS00410 at 89.607 kb on + strand at 89.896 kb on + strand at 89.896 kb on + strand at 89.896 kb on + strand at 89.896 kb on + strand at 89.896 kb on + strand at 89.897 kb on - strand at 89.897 kb on - strand at 89.897 kb on - strand at 89.897 kb on - strand at 89.897 kb on - strand at 89.897 kb on - strand at 89.897 kb on - strand at 89.897 kb on - strand at 89.897 kb on - strand at 89.897 kb on - strand at 89.897 kb on - strand at 90.000 kb on - strand at 90.000 kb on - strand at 90.014 kb on - strand at 90.246 kb on + strand, within LRK54_RS00420 at 90.247 kb on - strand, within LRK54_RS00420 at 90.247 kb on - strand, within LRK54_RS00420 at 90.247 kb on - strand, within LRK54_RS00420
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with nitrite 50 mM remove 88,347 + -0.3 88,347 + -1.5 88,348 - -0.6 88,348 - -0.4 88,348 - +1.0 88,767 - LRK54_RS00405 0.51 -0.4 88,953 + LRK54_RS00405 0.75 -0.4 88,953 + LRK54_RS00405 0.75 +1.6 88,953 + LRK54_RS00405 0.75 -0.0 88,953 + LRK54_RS00405 0.75 +0.2 88,953 + LRK54_RS00405 0.75 +1.0 88,953 + LRK54_RS00405 0.75 -1.8 88,953 + LRK54_RS00405 0.75 -1.1 88,953 + LRK54_RS00405 0.75 +1.6 88,953 + LRK54_RS00405 0.75 +0.2 88,953 + LRK54_RS00405 0.75 -0.3 88,953 + LRK54_RS00405 0.75 +2.6 88,953 + LRK54_RS00405 0.75 +0.2 88,953 + LRK54_RS00405 0.75 -0.0 88,953 + LRK54_RS00405 0.75 -0.4 88,953 + LRK54_RS00405 0.75 -0.0 88,953 + LRK54_RS00405 0.75 +0.6 88,953 + LRK54_RS00405 0.75 +0.2 88,954 - LRK54_RS00405 0.75 -1.3 88,954 - LRK54_RS00405 0.75 +0.2 88,954 - LRK54_RS00405 0.75 +1.3 88,954 - LRK54_RS00405 0.75 -3.1 88,954 - LRK54_RS00405 0.75 -2.3 88,954 - LRK54_RS00405 0.75 -1.6 88,954 - LRK54_RS00405 0.75 -0.6 88,954 - LRK54_RS00405 0.75 -0.4 88,954 - LRK54_RS00405 0.75 -1.0 88,954 - LRK54_RS00405 0.75 -1.9 88,954 - LRK54_RS00405 0.75 -0.6 88,954 - LRK54_RS00405 0.75 -2.8 88,954 - LRK54_RS00405 0.75 -1.1 88,954 - LRK54_RS00405 0.75 -0.8 88,954 - LRK54_RS00405 0.75 +1.0 88,954 - LRK54_RS00405 0.75 -0.6 88,954 - LRK54_RS00405 0.75 -0.6 88,954 - LRK54_RS00405 0.75 +0.2 88,954 - LRK54_RS00405 0.75 -3.1 88,954 - LRK54_RS00405 0.75 -1.0 89,012 - LRK54_RS00405 0.83 -0.4 89,071 + -1.4 89,071 + -1.6 89,134 + -0.0 89,135 - -1.4 89,135 - -1.7 89,171 + -0.8 89,171 + -1.1 89,171 + -1.8 89,171 + -1.0 89,171 + -0.9 89,171 + -1.0 89,171 + -1.6 89,171 + +0.7 89,171 + -0.4 89,171 + -1.0 89,171 + -1.0 89,171 + -1.8 89,172 - -0.7 89,172 - +1.4 89,172 - +0.4 89,172 - -0.5 89,172 - +0.6 89,172 - +0.7 89,172 - -0.9 89,172 - -0.4 89,172 - -0.8 89,172 - +0.6 89,172 - -0.3 89,172 - +0.8 89,172 - +0.6 89,172 - +1.2 89,172 - -0.1 89,172 - -1.8 89,172 - -0.8 89,172 - +0.4 89,172 - -1.1 89,172 - +0.3 89,172 - -1.4 89,172 - -0.0 89,267 + -0.5 89,267 + -1.4 89,267 + -0.1 89,267 + +0.1 89,267 + -0.4 89,267 + -0.9 89,267 + -0.9 89,267 + -0.1 89,267 + -1.0 89,267 + +0.1 89,267 + -0.3 89,267 + -0.4 89,267 + +1.4 89,267 + -0.9 89,267 + -0.3 89,267 + +0.7 89,267 + -0.1 89,268 - -0.8 89,268 - +0.2 89,268 - -0.8 89,268 - -1.0 89,268 - +0.6 89,268 - +1.0 89,268 - -1.3 89,268 - -1.7 89,268 - +0.4 89,268 - -0.9 89,268 - -2.1 89,268 - +1.0 89,268 - -0.6 89,268 - +1.0 89,268 - +0.6 89,268 - -0.1 89,268 - -1.3 89,268 - -0.3 89,268 - -0.4 89,268 - -0.8 89,268 - +1.2 89,282 - -0.0 89,282 - -1.8 89,282 - -2.0 89,355 + LRK54_RS00410 0.21 -0.4 89,355 + LRK54_RS00410 0.21 -0.0 89,356 - LRK54_RS00410 0.21 -0.7 89,356 - LRK54_RS00410 0.21 -0.5 89,607 + +1.2 89,896 + -0.6 89,896 + -0.8 89,896 + -0.1 89,896 + +0.1 89,896 + +0.4 89,897 - -2.2 89,897 - -0.4 89,897 - -0.8 89,897 - -0.0 89,897 - -0.5 89,897 - -0.7 89,897 - +0.6 89,897 - +0.1 89,897 - -1.1 89,897 - -1.0 89,897 - +1.0 90,000 - -0.7 90,000 - -0.7 90,014 - +0.3 90,246 + LRK54_RS00420 0.10 -1.8 90,247 - LRK54_RS00420 0.10 -0.6 90,247 - LRK54_RS00420 0.10 +1.0 90,247 - LRK54_RS00420 0.10 -0.1
Or see this region's nucleotide sequence