Experiment: H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS04975 and MMJJ_RS04980 are separated by 15 nucleotides MMJJ_RS04980 and MMJJ_RS04985 are separated by 4 nucleotides
MMJJ_RS04975: MMJJ_RS04975 - glycosyltransferase family 4 protein, at 923,835 to 924,902
_RS04975
MMJJ_RS04980: MMJJ_RS04980 - bifunctional precorrin-2 dehydrogenase/sirohydrochlorin ferrochelatase, at 924,918 to 925,523
_RS04980
MMJJ_RS04985: MMJJ_RS04985 - glutamyl-tRNA reductase, at 925,528 to 926,676
_RS04985
Position (kb) 924
925
926 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 924.025 kb on - strand, within MMJJ_RS04975 at 924.112 kb on + strand, within MMJJ_RS04975 at 924.196 kb on - strand, within MMJJ_RS04975 at 924.265 kb on - strand, within MMJJ_RS04975 at 924.726 kb on - strand, within MMJJ_RS04975 at 924.875 kb on - strand at 924.875 kb on - strand at 924.877 kb on - strand at 924.877 kb on - strand at 924.878 kb on + strand at 924.878 kb on + strand at 924.897 kb on + strand at 924.897 kb on + strand at 924.951 kb on + strand at 925.011 kb on + strand, within MMJJ_RS04980 at 925.058 kb on - strand, within MMJJ_RS04980 at 925.099 kb on - strand at 925.108 kb on - strand, within MMJJ_RS04980 at 925.123 kb on - strand, within MMJJ_RS04980 at 925.123 kb on - strand, within MMJJ_RS04980 at 925.124 kb on + strand, within MMJJ_RS04980 at 925.148 kb on - strand, within MMJJ_RS04980 at 925.149 kb on + strand, within MMJJ_RS04980 at 925.152 kb on + strand, within MMJJ_RS04980 at 925.209 kb on + strand, within MMJJ_RS04980 at 925.300 kb on - strand, within MMJJ_RS04980 at 925.302 kb on - strand, within MMJJ_RS04980 at 925.303 kb on + strand, within MMJJ_RS04980 at 925.469 kb on - strand at 925.661 kb on + strand, within MMJJ_RS04985 at 925.676 kb on - strand, within MMJJ_RS04985 at 925.677 kb on + strand, within MMJJ_RS04985 at 925.696 kb on + strand, within MMJJ_RS04985 at 925.788 kb on + strand, within MMJJ_RS04985 at 926.006 kb on - strand, within MMJJ_RS04985 at 926.098 kb on - strand, within MMJJ_RS04985 at 926.237 kb on - strand, within MMJJ_RS04985 at 926.270 kb on - strand, within MMJJ_RS04985 at 926.329 kb on + strand, within MMJJ_RS04985 at 926.332 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2 remove 924,025 - MMJJ_RS04975 0.18 -1.5 924,112 + MMJJ_RS04975 0.26 -1.7 924,196 - MMJJ_RS04975 0.34 -1.5 924,265 - MMJJ_RS04975 0.40 -1.9 924,726 - MMJJ_RS04975 0.83 -1.5 924,875 - +0.3 924,875 - -1.5 924,877 - -1.5 924,877 - +1.0 924,878 + +1.3 924,878 + +2.8 924,897 + -0.6 924,897 + -0.0 924,951 + -1.7 925,011 + MMJJ_RS04980 0.15 -3.1 925,058 - MMJJ_RS04980 0.23 -2.7 925,099 - -0.3 925,108 - MMJJ_RS04980 0.31 -1.4 925,123 - MMJJ_RS04980 0.34 -0.7 925,123 - MMJJ_RS04980 0.34 -2.5 925,124 + MMJJ_RS04980 0.34 -1.5 925,148 - MMJJ_RS04980 0.38 -3.3 925,149 + MMJJ_RS04980 0.38 -1.1 925,152 + MMJJ_RS04980 0.39 -1.7 925,209 + MMJJ_RS04980 0.48 -2.8 925,300 - MMJJ_RS04980 0.63 -0.3 925,302 - MMJJ_RS04980 0.63 -1.5 925,303 + MMJJ_RS04980 0.64 -2.6 925,469 - -0.3 925,661 + MMJJ_RS04985 0.12 -1.5 925,676 - MMJJ_RS04985 0.13 -2.1 925,677 + MMJJ_RS04985 0.13 -1.9 925,696 + MMJJ_RS04985 0.15 -2.3 925,788 + MMJJ_RS04985 0.23 -3.1 926,006 - MMJJ_RS04985 0.42 -1.3 926,098 - MMJJ_RS04985 0.50 +0.4 926,237 - MMJJ_RS04985 0.62 -2.1 926,270 - MMJJ_RS04985 0.65 -0.3 926,329 + MMJJ_RS04985 0.70 -1.1 926,332 + -1.3
Or see this region's nucleotide sequence