Strain Fitness in Shewanella sp. ANA-3 around Shewana3_2149

Experiment: nitrite 7.5 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntShewana3_2147 and Shewana3_2148 are separated by 70 nucleotidesShewana3_2148 and Shewana3_2149 are separated by 20 nucleotidesShewana3_2149 and Shewana3_2150 are separated by 12 nucleotidesShewana3_2150 and Shewana3_2151 are separated by 9 nucleotides Shewana3_2147: Shewana3_2147 - deoxyguanosinetriphosphate triphosphohydrolase-like protein (RefSeq), at 2,571,878 to 2,573,218 _2147 Shewana3_2148: Shewana3_2148 - keto-hydroxyglutarate-aldolase/keto-deoxy- phosphogluconate aldolase (RefSeq), at 2,573,289 to 2,573,930 _2148 Shewana3_2149: Shewana3_2149 - phosphogluconate dehydratase (RefSeq), at 2,573,951 to 2,575,777 _2149 Shewana3_2150: Shewana3_2150 - 6-phosphogluconolactonase (RefSeq), at 2,575,790 to 2,576,488 _2150 Shewana3_2151: Shewana3_2151 - glucose-6-phosphate 1-dehydrogenase (RefSeq), at 2,576,498 to 2,577,970 _2151 Position (kb) 2573 2574 2575 2576Strain fitness (log2 ratio) -3 -2 -1 0 1at 2572.996 kb on - strand, within Shewana3_2147at 2573.153 kb on + strandat 2574.181 kb on - strand, within Shewana3_2149at 2574.332 kb on - strand, within Shewana3_2149at 2574.602 kb on - strand, within Shewana3_2149at 2575.181 kb on - strand, within Shewana3_2149at 2575.445 kb on - strand, within Shewana3_2149at 2575.805 kb on - strandat 2576.006 kb on - strand, within Shewana3_2150at 2576.242 kb on - strand, within Shewana3_2150at 2576.373 kb on - strand, within Shewana3_2150

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Per-strain Table

Position Strand Gene LocusTag Fraction nitrite 7.5 mM
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2,572,996 - Shewana3_2147 0.83 -0.3
2,573,153 + +0.5
2,574,181 - Shewana3_2149 0.13 -0.3
2,574,332 - Shewana3_2149 0.21 +1.3
2,574,602 - Shewana3_2149 0.36 -0.7
2,575,181 - Shewana3_2149 0.67 -0.3
2,575,445 - Shewana3_2149 0.82 +1.3
2,575,805 - +0.2
2,576,006 - Shewana3_2150 0.31 -2.9
2,576,242 - Shewana3_2150 0.65 -2.7
2,576,373 - Shewana3_2150 0.83 -0.7

Or see this region's nucleotide sequence