Experiment: nitrite 7.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_2147 and Shewana3_2148 are separated by 70 nucleotides Shewana3_2148 and Shewana3_2149 are separated by 20 nucleotides Shewana3_2149 and Shewana3_2150 are separated by 12 nucleotides Shewana3_2150 and Shewana3_2151 are separated by 9 nucleotides
Shewana3_2147: Shewana3_2147 - deoxyguanosinetriphosphate triphosphohydrolase-like protein (RefSeq), at 2,571,878 to 2,573,218
_2147
Shewana3_2148: Shewana3_2148 - keto-hydroxyglutarate-aldolase/keto-deoxy- phosphogluconate aldolase (RefSeq), at 2,573,289 to 2,573,930
_2148
Shewana3_2149: Shewana3_2149 - phosphogluconate dehydratase (RefSeq), at 2,573,951 to 2,575,777
_2149
Shewana3_2150: Shewana3_2150 - 6-phosphogluconolactonase (RefSeq), at 2,575,790 to 2,576,488
_2150
Shewana3_2151: Shewana3_2151 - glucose-6-phosphate 1-dehydrogenase (RefSeq), at 2,576,498 to 2,577,970
_2151
Position (kb)
2573
2574
2575
2576 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2572.996 kb on - strand, within Shewana3_2147 at 2573.153 kb on + strand at 2574.181 kb on - strand, within Shewana3_2149 at 2574.332 kb on - strand, within Shewana3_2149 at 2574.602 kb on - strand, within Shewana3_2149 at 2575.181 kb on - strand, within Shewana3_2149 at 2575.445 kb on - strand, within Shewana3_2149 at 2575.805 kb on - strand at 2576.006 kb on - strand, within Shewana3_2150 at 2576.242 kb on - strand, within Shewana3_2150 at 2576.373 kb on - strand, within Shewana3_2150
Per-strain Table
Position Strand Gene LocusTag Fraction nitrite 7.5 mM remove 2,572,996 - Shewana3_2147 0.83 -0.3 2,573,153 + +0.5 2,574,181 - Shewana3_2149 0.13 -0.3 2,574,332 - Shewana3_2149 0.21 +1.3 2,574,602 - Shewana3_2149 0.36 -0.7 2,575,181 - Shewana3_2149 0.67 -0.3 2,575,445 - Shewana3_2149 0.82 +1.3 2,575,805 - +0.2 2,576,006 - Shewana3_2150 0.31 -2.9 2,576,242 - Shewana3_2150 0.65 -2.7 2,576,373 - Shewana3_2150 0.83 -0.7
Or see this region's nucleotide sequence