Experiment: Chlorite 200 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_1287 and Shew_1288 are separated by 32 nucleotides Shew_1288 and Shew_1289 overlap by 1 nucleotides Shew_1289 and ispG are separated by 19 nucleotides
Shew_1287: Shew_1287 - hypothetical protein (RefSeq), at 1,487,227 to 1,488,348
_1287
Shew_1288: Shew_1288 - type IV pilus biogenesis/stability protein PilW (RefSeq), at 1,488,381 to 1,489,169
_1288
Shew_1289: Shew_1289 - hypothetical protein (RefSeq), at 1,489,169 to 1,490,179
_1289
Shew_1290: ispG - 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (RefSeq), at 1,490,199 to 1,491,314
ispG
Position (kb) 1489
1490
1491 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 1488.474 kb on + strand, within Shew_1288 at 1489.069 kb on + strand, within Shew_1288 at 1489.156 kb on - strand at 1489.426 kb on + strand, within Shew_1289 at 1489.593 kb on + strand, within Shew_1289 at 1489.800 kb on - strand, within Shew_1289 at 1489.808 kb on - strand, within Shew_1289 at 1489.928 kb on - strand, within Shew_1289
Per-strain Table
Position Strand Gene LocusTag Fraction Chlorite 200 mM remove 1,488,474 + Shew_1288 0.12 -3.3 1,489,069 + Shew_1288 0.87 -0.3 1,489,156 - -2.8 1,489,426 + Shew_1289 0.25 -5.0 1,489,593 + Shew_1289 0.42 -3.5 1,489,800 - Shew_1289 0.62 +0.1 1,489,808 - Shew_1289 0.63 +0.0 1,489,928 - Shew_1289 0.75 -0.5
Or see this region's nucleotide sequence