Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_757 and Pf1N1B4_758 are separated by 316 nucleotides Pf1N1B4_758 and Pf1N1B4_759 are separated by 197 nucleotides Pf1N1B4_759 and Pf1N1B4_760 are separated by 97 nucleotides
Pf1N1B4_757 - phage-related integrase, at 860,562 to 861,812
_757
Pf1N1B4_758 - Bacterioferritin, at 862,129 to 862,602
_758
Pf1N1B4_759 - Glutaredoxin-related protein, at 862,800 to 863,141
_759
Pf1N1B4_760 - Formate dehydrogenase-O, major subunit (EC 1.2.1.2), at 863,239 to 865,344
_760
Position (kb)
862
863 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 861.188 kb on + strand, within Pf1N1B4_757 at 861.189 kb on - strand, within Pf1N1B4_757 at 861.284 kb on + strand, within Pf1N1B4_757 at 861.284 kb on + strand, within Pf1N1B4_757 at 861.285 kb on - strand, within Pf1N1B4_757 at 861.285 kb on - strand, within Pf1N1B4_757 at 861.285 kb on - strand, within Pf1N1B4_757 at 861.285 kb on - strand, within Pf1N1B4_757 at 861.285 kb on - strand, within Pf1N1B4_757 at 861.285 kb on - strand, within Pf1N1B4_757 at 861.285 kb on - strand, within Pf1N1B4_757 at 861.293 kb on + strand, within Pf1N1B4_757 at 861.293 kb on + strand, within Pf1N1B4_757 at 861.294 kb on - strand, within Pf1N1B4_757 at 861.398 kb on + strand, within Pf1N1B4_757 at 861.398 kb on + strand, within Pf1N1B4_757 at 861.399 kb on - strand, within Pf1N1B4_757 at 861.419 kb on - strand, within Pf1N1B4_757 at 861.419 kb on - strand, within Pf1N1B4_757 at 861.481 kb on + strand, within Pf1N1B4_757 at 861.482 kb on - strand, within Pf1N1B4_757 at 861.590 kb on + strand, within Pf1N1B4_757 at 861.608 kb on + strand, within Pf1N1B4_757 at 861.608 kb on + strand, within Pf1N1B4_757 at 861.608 kb on + strand, within Pf1N1B4_757 at 861.711 kb on + strand at 861.712 kb on - strand at 861.734 kb on - strand at 861.736 kb on + strand at 861.737 kb on - strand at 861.890 kb on - strand at 861.897 kb on + strand at 861.897 kb on + strand at 861.897 kb on + strand at 861.898 kb on - strand at 861.898 kb on - strand at 861.905 kb on - strand at 861.905 kb on - strand at 861.906 kb on + strand at 861.906 kb on + strand at 861.907 kb on - strand at 861.907 kb on - strand at 861.907 kb on - strand at 861.907 kb on - strand at 861.907 kb on - strand at 861.907 kb on - strand at 861.907 kb on - strand at 861.907 kb on - strand at 861.907 kb on - strand at 861.907 kb on - strand at 861.907 kb on - strand at 861.912 kb on + strand at 861.916 kb on - strand at 861.916 kb on - strand at 861.916 kb on - strand at 861.916 kb on - strand at 861.916 kb on - strand at 862.044 kb on - strand at 862.058 kb on + strand at 862.058 kb on + strand at 862.058 kb on + strand at 862.059 kb on - strand at 862.078 kb on - strand at 862.100 kb on + strand at 862.100 kb on + strand at 862.100 kb on + strand at 862.101 kb on - strand at 862.101 kb on - strand at 862.109 kb on - strand at 862.109 kb on - strand at 862.143 kb on + strand at 862.201 kb on + strand, within Pf1N1B4_758 at 862.201 kb on + strand, within Pf1N1B4_758 at 862.222 kb on + strand, within Pf1N1B4_758 at 862.222 kb on + strand, within Pf1N1B4_758 at 862.222 kb on + strand, within Pf1N1B4_758 at 862.222 kb on + strand, within Pf1N1B4_758 at 862.222 kb on + strand, within Pf1N1B4_758 at 862.222 kb on + strand, within Pf1N1B4_758 at 862.222 kb on + strand, within Pf1N1B4_758 at 862.223 kb on - strand, within Pf1N1B4_758 at 862.223 kb on - strand, within Pf1N1B4_758 at 862.223 kb on - strand at 862.261 kb on + strand, within Pf1N1B4_758 at 862.573 kb on + strand at 862.573 kb on + strand at 862.574 kb on - strand at 862.574 kb on - strand at 862.574 kb on - strand at 862.600 kb on + strand at 862.600 kb on + strand at 862.601 kb on - strand at 862.607 kb on + strand at 862.629 kb on + strand at 862.630 kb on - strand at 862.649 kb on + strand at 862.650 kb on - strand at 862.651 kb on + strand at 862.667 kb on + strand at 862.730 kb on + strand at 862.744 kb on + strand at 862.744 kb on + strand at 862.744 kb on + strand at 862.744 kb on + strand at 862.744 kb on + strand at 862.744 kb on + strand at 862.745 kb on - strand at 862.745 kb on - strand at 862.745 kb on - strand at 862.952 kb on - strand, within Pf1N1B4_759 at 863.000 kb on - strand, within Pf1N1B4_759 at 863.086 kb on + strand, within Pf1N1B4_759 at 863.087 kb on - strand, within Pf1N1B4_759 at 863.194 kb on + strand at 863.194 kb on + strand at 863.567 kb on + strand, within Pf1N1B4_760 at 863.567 kb on + strand, within Pf1N1B4_760 at 863.568 kb on - strand at 863.568 kb on - strand, within Pf1N1B4_760 at 863.568 kb on - strand, within Pf1N1B4_760 at 863.568 kb on - strand, within Pf1N1B4_760 at 863.568 kb on - strand, within Pf1N1B4_760
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 861,188 + Pf1N1B4_757 0.50 +0.2 861,189 - Pf1N1B4_757 0.50 +0.6 861,284 + Pf1N1B4_757 0.58 -0.0 861,284 + Pf1N1B4_757 0.58 -1.6 861,285 - Pf1N1B4_757 0.58 -0.3 861,285 - Pf1N1B4_757 0.58 -2.0 861,285 - Pf1N1B4_757 0.58 +0.4 861,285 - Pf1N1B4_757 0.58 +0.5 861,285 - Pf1N1B4_757 0.58 +0.2 861,285 - Pf1N1B4_757 0.58 -1.0 861,285 - Pf1N1B4_757 0.58 -0.0 861,293 + Pf1N1B4_757 0.58 +0.7 861,293 + Pf1N1B4_757 0.58 +1.0 861,294 - Pf1N1B4_757 0.59 -2.0 861,398 + Pf1N1B4_757 0.67 +0.4 861,398 + Pf1N1B4_757 0.67 +2.4 861,399 - Pf1N1B4_757 0.67 -0.6 861,419 - Pf1N1B4_757 0.69 +0.1 861,419 - Pf1N1B4_757 0.69 +2.0 861,481 + Pf1N1B4_757 0.73 -0.6 861,482 - Pf1N1B4_757 0.74 -0.0 861,590 + Pf1N1B4_757 0.82 +1.0 861,608 + Pf1N1B4_757 0.84 +0.7 861,608 + Pf1N1B4_757 0.84 +1.6 861,608 + Pf1N1B4_757 0.84 +0.6 861,711 + +0.8 861,712 - +1.6 861,734 - -0.0 861,736 + +1.0 861,737 - -0.0 861,890 - +0.7 861,897 + -0.7 861,897 + -0.6 861,897 + +0.3 861,898 - -0.8 861,898 - +1.6 861,905 - +0.0 861,905 - +1.0 861,906 + -1.0 861,906 + +1.6 861,907 - +0.6 861,907 - -0.2 861,907 - +0.7 861,907 - +0.0 861,907 - +0.4 861,907 - +0.5 861,907 - +1.0 861,907 - +1.0 861,907 - -0.3 861,907 - +1.3 861,907 - +0.5 861,912 + -0.4 861,916 - -0.2 861,916 - +2.0 861,916 - +0.2 861,916 - -0.4 861,916 - +0.7 862,044 - +2.8 862,058 + -2.3 862,058 + +0.3 862,058 + +0.5 862,059 - +1.3 862,078 - +1.9 862,100 + -0.4 862,100 + +1.2 862,100 + +0.0 862,101 - +0.7 862,101 - -1.6 862,109 - +2.6 862,109 - -0.6 862,143 + +2.3 862,201 + Pf1N1B4_758 0.15 +0.3 862,201 + Pf1N1B4_758 0.15 +0.8 862,222 + Pf1N1B4_758 0.20 +1.2 862,222 + Pf1N1B4_758 0.20 +2.5 862,222 + Pf1N1B4_758 0.20 +0.0 862,222 + Pf1N1B4_758 0.20 +0.5 862,222 + Pf1N1B4_758 0.20 -2.6 862,222 + Pf1N1B4_758 0.20 +0.0 862,222 + Pf1N1B4_758 0.20 +1.1 862,223 - Pf1N1B4_758 0.20 +0.3 862,223 - Pf1N1B4_758 0.20 -1.0 862,223 - -2.0 862,261 + Pf1N1B4_758 0.28 +0.0 862,573 + +0.0 862,573 + -1.6 862,574 - +1.6 862,574 - +1.0 862,574 - +0.6 862,600 + -1.0 862,600 + +0.3 862,601 - +1.6 862,607 + +0.5 862,629 + +1.6 862,630 - -0.6 862,649 + +0.4 862,650 - +0.7 862,651 + +0.0 862,667 + -0.3 862,730 + +2.3 862,744 + -1.0 862,744 + +0.0 862,744 + -0.7 862,744 + +0.0 862,744 + -0.8 862,744 + +1.0 862,745 - +0.4 862,745 - -0.7 862,745 - +0.7 862,952 - Pf1N1B4_759 0.44 +0.0 863,000 - Pf1N1B4_759 0.58 -0.6 863,086 + Pf1N1B4_759 0.84 -2.0 863,087 - Pf1N1B4_759 0.84 +0.0 863,194 + +1.0 863,194 + -1.0 863,567 + Pf1N1B4_760 0.16 +0.7 863,567 + Pf1N1B4_760 0.16 +0.7 863,568 - +0.6 863,568 - Pf1N1B4_760 0.16 -0.6 863,568 - Pf1N1B4_760 0.16 +3.0 863,568 - Pf1N1B4_760 0.16 -2.3 863,568 - Pf1N1B4_760 0.16 +0.4
Or see this region's nucleotide sequence