Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5885 and Pf1N1B4_5886 overlap by 4 nucleotides Pf1N1B4_5886 and Pf1N1B4_5887 are separated by 0 nucleotides Pf1N1B4_5887 and Pf1N1B4_5888 are separated by 22 nucleotides
Pf1N1B4_5885 - dTDP-glucose 4,6-dehydratase (EC 4.2.1.46), at 328,664 to 329,722
_5885
Pf1N1B4_5886 - Undecaprenyl-phosphate galactosephosphotransferase (EC 2.7.8.6), at 329,719 to 330,465
_5886
Pf1N1B4_5887 - Tyrosine-protein kinase Wzc (EC 2.7.10.2), at 330,466 to 331,110
_5887
Pf1N1B4_5888 - probable polysaccharide biosynthesis protein, at 331,133 to 332,155
_5888
Position (kb) 329
330
331 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 328.744 kb on - strand at 328.773 kb on - strand, within Pf1N1B4_5885 at 328.773 kb on - strand, within Pf1N1B4_5885 at 328.938 kb on - strand, within Pf1N1B4_5885 at 328.962 kb on - strand, within Pf1N1B4_5885 at 329.036 kb on + strand, within Pf1N1B4_5885 at 329.036 kb on + strand at 329.036 kb on + strand, within Pf1N1B4_5885 at 329.121 kb on - strand, within Pf1N1B4_5885 at 329.162 kb on + strand at 329.162 kb on + strand, within Pf1N1B4_5885 at 329.162 kb on + strand, within Pf1N1B4_5885 at 329.163 kb on - strand, within Pf1N1B4_5885 at 329.163 kb on - strand, within Pf1N1B4_5885 at 329.324 kb on + strand, within Pf1N1B4_5885 at 329.324 kb on + strand, within Pf1N1B4_5885 at 329.325 kb on - strand, within Pf1N1B4_5885 at 329.325 kb on - strand, within Pf1N1B4_5885 at 329.442 kb on - strand, within Pf1N1B4_5885 at 329.442 kb on - strand, within Pf1N1B4_5885 at 329.481 kb on - strand, within Pf1N1B4_5885 at 329.660 kb on + strand at 329.661 kb on - strand at 329.663 kb on + strand at 330.031 kb on + strand at 330.031 kb on + strand, within Pf1N1B4_5886 at 330.032 kb on - strand, within Pf1N1B4_5886 at 330.266 kb on - strand, within Pf1N1B4_5886 at 330.298 kb on + strand, within Pf1N1B4_5886 at 330.379 kb on + strand, within Pf1N1B4_5886 at 330.380 kb on - strand, within Pf1N1B4_5886 at 330.380 kb on - strand, within Pf1N1B4_5886 at 330.380 kb on - strand, within Pf1N1B4_5886 at 331.075 kb on + strand at 331.078 kb on + strand at 331.078 kb on + strand at 331.078 kb on + strand at 331.078 kb on + strand at 331.079 kb on - strand at 331.204 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 328,744 - +0.8 328,773 - Pf1N1B4_5885 0.10 -0.4 328,773 - Pf1N1B4_5885 0.10 -1.2 328,938 - Pf1N1B4_5885 0.26 -2.0 328,962 - Pf1N1B4_5885 0.28 +1.1 329,036 + Pf1N1B4_5885 0.35 +0.2 329,036 + +0.6 329,036 + Pf1N1B4_5885 0.35 -0.4 329,121 - Pf1N1B4_5885 0.43 +0.0 329,162 + -1.7 329,162 + Pf1N1B4_5885 0.47 -0.2 329,162 + Pf1N1B4_5885 0.47 -0.1 329,163 - Pf1N1B4_5885 0.47 +0.6 329,163 - Pf1N1B4_5885 0.47 +0.9 329,324 + Pf1N1B4_5885 0.62 -0.1 329,324 + Pf1N1B4_5885 0.62 -0.4 329,325 - Pf1N1B4_5885 0.62 -0.1 329,325 - Pf1N1B4_5885 0.62 -0.4 329,442 - Pf1N1B4_5885 0.73 -1.0 329,442 - Pf1N1B4_5885 0.73 -0.9 329,481 - Pf1N1B4_5885 0.77 -2.2 329,660 + -0.4 329,661 - +1.7 329,663 + +1.1 330,031 + +0.1 330,031 + Pf1N1B4_5886 0.42 +1.0 330,032 - Pf1N1B4_5886 0.42 +3.7 330,266 - Pf1N1B4_5886 0.73 -0.4 330,298 + Pf1N1B4_5886 0.78 +0.4 330,379 + Pf1N1B4_5886 0.88 +0.3 330,380 - Pf1N1B4_5886 0.88 +0.2 330,380 - Pf1N1B4_5886 0.88 +1.6 330,380 - Pf1N1B4_5886 0.88 +0.5 331,075 + -0.4 331,078 + -0.4 331,078 + -1.4 331,078 + +0.1 331,078 + +0.6 331,079 - -0.4 331,204 + -2.0
Or see this region's nucleotide sequence