Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5803 and Pf1N1B4_5804 are separated by 51 nucleotides Pf1N1B4_5804 and Pf1N1B4_5805 are separated by 10 nucleotides
Pf1N1B4_5803 - Catechol 1,2-dioxygenase (EC 1.13.11.1), at 237,971 to 238,900
_5803
Pf1N1B4_5804 - Muconolactone isomerase (EC 5.3.3.4), at 238,952 to 239,242
_5804
Pf1N1B4_5805 - Muconate cycloisomerase (EC 5.5.1.1), at 239,253 to 240,542
_5805
Position (kb) 238
239
240 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 238.146 kb on + strand, within Pf1N1B4_5803 at 238.147 kb on - strand at 238.167 kb on + strand at 238.167 kb on + strand, within Pf1N1B4_5803 at 238.167 kb on + strand, within Pf1N1B4_5803 at 238.168 kb on - strand, within Pf1N1B4_5803 at 238.168 kb on - strand, within Pf1N1B4_5803 at 238.413 kb on + strand, within Pf1N1B4_5803 at 238.572 kb on + strand, within Pf1N1B4_5803 at 238.572 kb on + strand, within Pf1N1B4_5803 at 238.654 kb on - strand, within Pf1N1B4_5803 at 238.654 kb on - strand, within Pf1N1B4_5803 at 238.719 kb on + strand, within Pf1N1B4_5803 at 238.720 kb on - strand, within Pf1N1B4_5803 at 238.835 kb on - strand at 238.918 kb on + strand at 238.918 kb on + strand at 238.923 kb on - strand at 239.016 kb on + strand, within Pf1N1B4_5804 at 239.085 kb on + strand at 239.085 kb on + strand, within Pf1N1B4_5804 at 239.094 kb on + strand, within Pf1N1B4_5804 at 239.094 kb on + strand, within Pf1N1B4_5804 at 239.114 kb on + strand, within Pf1N1B4_5804
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 238,146 + Pf1N1B4_5803 0.19 +0.3 238,147 - +0.9 238,167 + -2.5 238,167 + Pf1N1B4_5803 0.21 +0.4 238,167 + Pf1N1B4_5803 0.21 -1.3 238,168 - Pf1N1B4_5803 0.21 -0.7 238,168 - Pf1N1B4_5803 0.21 -0.1 238,413 + Pf1N1B4_5803 0.48 -1.5 238,572 + Pf1N1B4_5803 0.65 -1.5 238,572 + Pf1N1B4_5803 0.65 +0.9 238,654 - Pf1N1B4_5803 0.73 -1.8 238,654 - Pf1N1B4_5803 0.73 +0.5 238,719 + Pf1N1B4_5803 0.80 +0.5 238,720 - Pf1N1B4_5803 0.81 +0.5 238,835 - -2.8 238,918 + +1.1 238,918 + +0.1 238,923 - -0.5 239,016 + Pf1N1B4_5804 0.22 +1.1 239,085 + -0.5 239,085 + Pf1N1B4_5804 0.46 +0.9 239,094 + Pf1N1B4_5804 0.49 -0.5 239,094 + Pf1N1B4_5804 0.49 +0.3 239,114 + Pf1N1B4_5804 0.56 +0.3
Or see this region's nucleotide sequence